SiRNA Validation Data



Gene ID:



NM_001207013.1 NM_001308287.1 NM_002828.3 NM_080422.2 NM_080423.2 XM_005258124.3 XM_005258125.3 XM_011525705.2 XM_011525706.2 XM_011525707.2 XM_017025884.1 XM_017025885.1 XM_017025886.1 XM_017025887.1 XM_017025888.1

Validation Results:

18%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00747429_mH]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.