SiRNA Validation Data



Gene ID:



NM_001243027.2 NM_001243028.2 NM_001626.5 XM_011526614.1 XM_011526615.1 XM_011526616.1 XM_011526618.1 XM_011526619.1 XM_011526620.1 XM_011526622.2 XM_017026470.1 XM_017026471.1

Validation Results:

7%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00609846_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.