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SiRNA Validation Data

siRNA ID:

s27660

Gene ID:

51196

Transcript(s):

NM_001165979.2 NM_001288989.1 NM_016341.3 XM_006717885.3 XM_006717888.3 XM_006717889.3 XM_006717890.2 XM_011539849.2 XM_011539850.2 XM_011539851.2 XM_011539852.2 XM_017016310.1 XM_017016311.1 XM_017016312.1

Validation Results:

17%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00275279_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.