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SiRNA Validation Data

siRNA ID:

s28167

Gene ID:

51455

Transcript(s):

NM_001037872.2 NM_001321454.1 NM_001321455.1 NM_001321458.1 NM_001321459.1 NM_001321460.1 NM_016316.3 NR_135649.1 NR_135650.1 NR_135651.1 NR_135652.1 NR_135653.1 XM_017004309.1 XM_017004310.1 XM_017004311.1 XM_017004312.1 XM_017004313.1

Validation Results:

20%(+/-8% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00249411_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.