SiRNA Validation Data



Gene ID:



NM_024586.5 NM_148904.3 NM_148905.3 NM_148906.2 NM_148907.2 NM_148908.3 NM_148909.3 NR_036662.1 XM_006710318.2 XM_006710320.3 XM_006710321.2 XM_006710322.3 XM_006710323.2 XM_006710324.3 XM_006710325.3 XM_006710326.2 XM_011540599.2 XM_011540600.2 XM_011540601.2 XM_011540602.2 XM_011540603.2 XM_011540604.2 XM_017000218.1 XM_017000219.1 XM_017000220.1 XM_017000221.1 XM_017000222.1 XM_017000223.1 XM_017000224.1 XM_017000225.1

Validation Results:

11%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00541027_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.