SiRNA Validation Data

siRNA ID:

s6186

Gene ID:

2908

Transcript(s):

NM_000176.2 NM_001018074.1 NM_001018075.1 NM_001018076.1 NM_001018077.1 NM_001020825.1 NM_001024094.1 NM_001204258.1 NM_001204259.1 NM_001204260.1 NM_001204261.1 NM_001204262.1 NM_001204263.1 NM_001204264.1 NM_001204265.1 XM_005268419.3 XM_005268420.4 XM_005268422.3 XM_005268423.3 XM_017009397.1 XM_017009398.1

Validation Results:

15%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00230813_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.