SiRNA Validation Data

siRNA ID:

s659

Gene ID:

207

Transcript(s):

NM_001014431.1 NM_001014432.1 NM_005163.2 XM_005267401.1 XM_017021075.1 XM_017021076.1 XM_017021077.1 XM_017021078.1

Validation Results:

10%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00178289_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.