SiRNA Validation Data



Gene ID:



NM_003150.3 NM_139276.2 NM_213662.1 XM_005257616.3 XM_005257617.3 XM_011525145.2 XM_011525146.2 XM_017024972.1 XM_017024973.1 XM_017024974.1 XM_017024975.1 XM_017024976.1

Validation Results:

13%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00374280_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.