SiRNA Validation Data

siRNA ID:

s11070

Gene ID:

5567

Transcript(s):

NM_001242857.2 NM_001242858.2 NM_001242859.2 NM_001242860.2 NM_001242861.2 NM_001242862.2 NM_001300915.1 NM_001300916.1 NM_001300917.1 NM_002731.3 NM_182948.3 NM_207578.2 XM_005271015.3 XM_005271016.1 XM_005271017.3 XM_005271018.4 XM_005271019.1 XM_005271020.1 XM_006710758.1 XM_017001706.1 XM_017001707.1 XM_017001708.1 XM_017001709.1 XM_017001710.1 XM_017001711.1 XM_017001712.1 XM_017001713.1 XM_017001714.1 XM_017001715.1 XM_017001716.1 XM_017001717.1 XM_017001718.1 XM_017001719.1 XM_017001720.1

Validation Results:

12%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00176944_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.