SiRNA Validation Data



Gene ID:



NM_000276.3 NM_001318784.1 NM_001587.3 XM_005262422.2 XM_011531344.2 XM_011531345.2 XM_017029554.1

Validation Results:

15%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00240844_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.