SiRNA Validation Data



Gene ID:



NM_001242864.2 NM_001242865.2 NM_001242866.2 NM_001286676.1 NM_001286677.1 NM_001286678.1 NM_001535.4 NM_206962.3 XM_005261111.3 XM_006723998.3 XM_006723999.2 XM_006724000.2 XM_017028339.1 XM_017028340.1

Validation Results:

5%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00181759_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.