SiRNA Validation Data



Gene ID:



NM_000877.3 NM_001320978.1 NM_001320980.1 NM_001320981.1 NM_001320982.1 NM_001320983.1 NM_001320984.1 NM_001320985.1 XM_005263930.4 XM_005263934.4 XM_011511115.2 XM_011511116.1 XM_011511117.1 XM_011511118.2 XM_017003989.1

Validation Results:

14%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00168392_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.