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SiRNA Validation Data

siRNA ID:

s4125

Gene ID:

1741

Transcript(s):

NM_001166278.1 NM_020730.2 NM_021120.3 XM_005262248.3 XM_006724625.2 XM_006724626.2 XM_011530883.1 XM_017029322.1 XM_017029323.1 XM_017029324.1 XM_017029325.1 XM_017029326.1 XM_017029327.1

Validation Results:

5%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00221664_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.