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          • Primary Antibodies ›
          • HIF1A Antibodies

          Invitrogen

          HIF1A Recombinant Rabbit Monoclonal Antibody (HL3154)

          View all (89) HIF1A antibodies

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          Cite HIF1A Recombinant Rabbit Monoclonal Antibody (HL3154)

          Additional Information:
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          • Antibody Testing Data (4)
          HIF1A Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          HIF1A Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          HIF1A Antibody (MA5-56739) in ICC/IF

          Immunocytochemistry analysis of HIF1A using HIF1A Recombinant Monoclonal Antibody (Product # MA5-56739) in Mock and treated HeLa cells fixed in ice-cold MeOH for 5 min. HIF1A antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (Product # MA5-31466) . {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          HIF1A Antibody in Immunocytochemistry (ICC/IF)
          HIF1A Antibody in Western Blot (WB)
          HIF1A Antibody in Western Blot (WB)
          HIF1A Antibody in Western Blot (WB)

          Product Details

          MA5-56739

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3000
          -

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          HL3154

          Immunogen

          Recombinant fragment of human HIF1 alpha.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Dry ice (international)

          RRID

          AB_3679765

          Target Information

          HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: ARNT interacting protein; ARNT-interacting protein; ARNT2; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; hif 1; hif 1a; HIF-1 alpha; HIF-1-alpha; HIF-1alpha 786; HIF1 alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1 alpha; Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; Member of PAS protein 1; member of PAS superfamily 1; PAS domain-containing protein 8; transcription factor; transcription factor; hypoxia-inducible factor 1a; unnamed protein product

          View more View less

          Gene Aliases: AA959795; BHLHE78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; HIF1A; HIF1alpha; MOP1; PASD8

          View more View less

          UniProt ID: (Human) Q16665, (Mouse) Q61221, (Rat) O35800

          View more View less

          Entrez Gene ID: (Human) 3091, (Mouse) 15251, (Rat) 29560

          View more View less

          Function(s)
          RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding transcription corepressor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding p53 binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding ligand-dependent nuclear receptor binding enzyme binding protein kinase binding protein domain specific binding ubiquitin protein ligase binding histone deacetylase binding sequence-specific DNA binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding RNA polymerase II sequence-specific DNA binding transcription factor binding E-box binding transcription regulator activator activity transcription factor activity, transcription factor binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding signal transducer activity transcription factor binding protein complex binding histone acetyltransferase binding nuclear hormone receptor binding double-stranded DNA binding basic helix-loop-helix transcription factor
          Process(es)
          response to reactive oxygen species angiogenesis blood vessel development response to hypoxia cellular glucose homeostasis neural crest cell migration epithelial to mesenchymal transition embryonic placenta development B-1 B cell homeostasis positive regulation of endothelial cell proliferation vasculature development heart looping positive regulation of neuroblast proliferation chondrocyte differentiation glandular epithelial cell differentiation glandular epithelial cell maturation connective tissue replacement involved in inflammatory response wound healing outflow tract morphogenesis cardiac ventricle morphogenesis lactate metabolic process regulation of glycolytic process regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cellular iron ion homeostasis apoptotic process signal transduction neuroblast proliferation lactation visual learning response to iron ion regulation of gene expression vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration response to muscle activity positive regulation of macroautophagy axonal transport of mitochondrion neural fold elevation formation cerebral cortex development cell differentiation negative regulation of ossification bone mineralization negative regulation of bone mineralization positive regulation of vascular endothelial growth factor receptor signaling pathway TOR signaling negative regulation of TOR signaling oxygen homeostasis positive regulation of chemokine production regulation of transforming growth factor beta2 production collagen metabolic process cellular response to oxidative stress embryonic hemopoiesis insulin secretion involved in cellular response to glucose stimulus positive regulation of insulin secretion involved in cellular response to glucose stimulus regulation of cell proliferation hemoglobin biosynthetic process glucose homeostasis negative regulation of apoptotic process negative regulation of neuron apoptotic process positive regulation of blood vessel endothelial cell migration positive regulation of erythrocyte differentiation positive regulation of angiogenesis positive regulation of glycolytic process positive regulation of transcription, DNA-templated negative regulation of growth positive regulation of transcription from RNA polymerase II promoter muscle cell cellular homeostasis positive regulation of hormone biosynthetic process blood vessel morphogenesis digestive tract morphogenesis camera-type eye morphogenesis cartilage development neuron apoptotic process elastin metabolic process intestinal epithelial cell maturation epithelial cell differentiation involved in mammary gland alveolus development iris morphogenesis retina vasculature development in camera-type eye positive regulation of chemokine-mediated signaling pathway regulation of thymocyte apoptotic process negative regulation of thymocyte apoptotic process cellular response to interleukin-1 cellular response to hypoxia dopaminergic neuron differentiation mesenchymal cell apoptotic process hypoxia-inducible factor-1alpha signaling pathway cellular response to virus positive regulation of cytokine production involved in inflammatory response negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of nitric oxide metabolic process regulation of protein neddylation negative regulation of mesenchymal cell apoptotic process transcription, DNA-templated transcription from RNA polymerase II promoter positive regulation of cell proliferation positive regulation of autophagy positive regulation of receptor biosynthetic process negative regulation of transcription elongation from RNA polymerase II promoter positive regulation of apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress negative regulation of vasoconstriction positive regulation of smooth muscle cell proliferation regulation of catalytic activity response to fungicide positive regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of mitophagy regulation of aerobic respiration negative regulation of reactive oxygen species metabolic process response to mechanical stimulus response to salt stress response to glucose response to X-ray response to auditory stimulus response to purine-containing compound response to activity response to cobalt ion response to estradiol cellular response to insulin stimulus cellular response to drug response to drug response to alkaloid response to estrogen positive regulation of cell size response to glucocorticoid maternal process involved in female pregnancy cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to carbon monoxide cellular response to nitrite cellular response to electrical stimulus cellular response to mechanical stimulus cellular response to cobalt ion cellular response to glucose stimulus cellular response to lipid cellular response to organic cyclic compound cellular response to light stimulus response to anesthetic cellular response to toxic substance regulation of cellular response to hypoxia cellular response to cyanide
          It has to be done as per old AB suggested Products section.

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