|Flow Cytometry (Flow)||1:50-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol, 1% BSA|
|Contains||0.025% Proclin 300|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Recommended positive controls: A431, K562, MCF-7, AsPC-1, MDA-MB-231, MDA-MB-231 (24 µg/ml Tunicamycin for 16hr), U87-MG, U87-MG membrane extracts, U87-MG conditioned medium, Mouse brain, mouse fetal brain, A431 (500 units PNGase F for 4 hr), A431 (24 µg/ml Tunicamycin treatment for 16hr), A431 (4 mM Benzyl glucosinolate treatment for 32hr), A431(24 µg/ml Tunicamycin treatment for 16hr and 4 mM Benzyl glucosinolate treatment for 32hr).|Predicted reactivity: Mouse (89%), Rat (88%), Rhesus Monkey (97%), Bovine (89%).|Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: glypican; glypican proteoglycan 1; Glypican-1; GPI-anchored cell surface heparan sulfate proteoglycan; HSPG M12; Secreted glypican-1
Gene Aliases: AI462976; glypican; GPC1; HSPG M12; HSPGM12
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