Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
PEP-100 is a 15 amino acid synthetic peptide whose sequence corresponds to amino acid residues 249-263 from human proteasome 20S C2 subunit. The sequence of this peptide is (amino to carboxy terminus): P (249)-A-D-E-P-A-E-K-A-D-E-P-M-E-H (263)
This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and human proteasome 20S C2 subunit, catalog # PA1-963. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~70-fold) for competitive inhibition of antibody-protein binding reactions.
Reconstitute with 0.1 mL of distilled water.
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signaling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (approximately31), barrel-shaped molecular machine with an apparent molecular weight of approximately2. 5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.
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Protein Aliases: 30 kDa prosomal protein; epididymis secretory protein Li 275; Macropain subunit C2; macropain subunit nu; MGC14542; MGC14575; MGC14751; MGC1667; MGC21459; MGC22853; MGC23915; Multicatalytic endopeptidase complex subunit C2; PROS-30; proteasome (prosome, macropain) subunit, alpha type, 1; Proteasome component C2; Proteasome nu chain; Proteasome subunit alpha type-1; proteasome subunit nu; proteasome subunit, alpha-type, 1; protein P30-33K; testicular tissue protein Li 150
Gene Aliases: HC2; NU; PROS30; PSC2; PSMA1
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