Analytical sensitivity
0.14 ng/mL
Assay range
0.3-20 ng/mL
Sample type/volume
Plasma
10 µL
Serum
10 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 30 min
Homogenous (no wash)
No
Interassay CV
5.9%
Intraassay CV
5.9%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Storage
2-8°C
Protein name
Complement C3a
Protein aliases
Acylation stimulating protein, acylation-stimulating protein cleavage product, ASP, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3-beta-c, C3a anaphylatoxin, C3adesArg, C3bc, Complement C3, Complement C3 alpha chain, Complement C3 beta chain, Complement C3b alpha' chain, Complement C3c alpha' chain fragment 1, Complement C3c alpha' chain fragment 2, Complement C3d fragment, Complement C3dg fragment, Complement C3f fragment, Complement C3g fragment, complement component C3a, complement component C3b, epididymis secretory sperm binding protein Li 62p, prepro-C3
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
AHUS5, ARMD9, ASP, C3, C3a, C3b, CPAMD1, HEL-S-62p
Gene ID
Gene symbol
C3
UniProt ID
The Human Complement C3a (Hu C3a) ELISA quantitates Hu C3a in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu C3a.
Principle of the method
The Human C3a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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