Analytical sensitivity
0.08 ng/mL
Assay range
0.9-60 ng/mL
Sample type/volume
Plasma
10 µL
Serum
10 µL
Supernatant
10 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 30 min
Homogenous (no wash)
No
Interassay CV
4.2%
Intraassay CV
4.7%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Lysis Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Storage
2-8°C
Protein name
C1q
Protein aliases
complement C1q chain A, complement C1q chain B, Complement C1q subcomponent subunit A, Complement C1q subcomponent subunit B, Complement C1q subcomponent subunit C, complement component 1, q subcomponent, A chain, alpha polypeptide, B chain, beta polypeptide, C chain, gamma polypeptide, complement component C1q, complement subcomponent C1q chain B
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
C1Q-C, C1QA, C1QB, C1QC, C1QG
Gene ID
Gene symbol
C1QA, C1QB, C1QC
UniProt ID
The Human C1q ELISA quantitates Hu C1q in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu C1q.
Principle of the method
The Human C1q solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
C1q is a sub-component of complement C1. C1q associates with complement components C1r and C1s, which are required for the progression of the proteolytic complement cascade. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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