Analytical sensitivity
0.6 ng/mL
Assay range
3.15-100 ng/mL
Sample type/volume
Amniotic Fluid
100 µL
Plasma
10 µL
Serum
10 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 10 min
Homogenous (no wash)
No
Interassay CV
5.2%
Intraassay CV
3.1%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Storage
2-8°C
Protein name
VCAM-1 (CD106)
Target sub-specificity
Soluble
Protein aliases
CD106, CD106 antigen, INCAM-100, V-CAM 1, VCAM-1, Vascular cell adhesion protein 1
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
CD106, INCAM-100, VCAM1
Gene ID
Gene symbol
VCAM1
UniProt ID
The Human Vascular Cell Adhesion Molecula-1 (Hu VCAM-1) ELISA quantitates Hu VCAM-1 in human serum, plasma, amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VcAM-1.
Principle of the method
The Human VCAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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