Invitrogen
Analytical sensitivity
0.62 ng/mL
Assay range
0.62-150 ng/mL
Sample type/volume
Serum
50 µL
Supernatant
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping condition
Wet or Dry Ice
Storage
2-8°C
Protein name
CD100 (SEMA4D)
Protein aliases
A8, BB18, CD100, GR3, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D, 4D, Semaphorin-4D
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
C9orf164, CD100, coll-4, M-sema-G, SEMA4D, SEMAJ
Gene ID
Gene symbol
SEMA4D
UniProt ID
Human Semaphorin 4D quantitates human Semaphorin 4D in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Semaphorin 4D.
Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support
