The Human Albumin (Hu ALB) ELISA quantitates Hu ALB in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ALB.
Principle of the method
The Human ALB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Albumin is a soluble, monomeric protein with a molecular weight of approximately 65-67 kDa that comprises about one-half of the blood serum protein. Human serum albumin (HAS) is the most abundant protein in human blood plasma, and has a serum half-life of approximately 20 days. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular, unglycosylated serum protein synthesized in the liver as pre-pro-albumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough `produce the secreted albumin. Serum albumin has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. The main function of albumin is the regulation of the colloidal osmotic pressure of blood.
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.