Invitrogen
Analytical sensitivity
180 pg/mL
Assay range
187.5-12,000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping condition
Wet or Dry Ice
Storage
2-8°C
Protein name
EpoR
Protein aliases
EPO-R, Erythropoietin receptor
Protein family
Other Proteins
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
EPO-R, EPOR
Gene ID
Gene symbol
EPOR
UniProt ID
The Human EPO Receptor (EPO R) ELISA quantitates Hu EPO R in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu EPO R.
Principle of the method
The Human EPO R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Recombinant Human Erythropoietin Receptor (EPO R) is a type I membrane-spanning protein, expressed in a mouse myeloma cell line, NSO. This product is produced from a cDNA sequence encoding the extracellular domain of the EPO receptor. The 225 amino acid residue mature recombinant soluble EPO receptor, generated after cleavage of a 25 amino acid residue signal peptide, has predicted molecular mass of approximately 25 kDa.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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