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GeneArt® Primer and Construct Design Tool

GeneArt® Seamless Cloning and Assembly Kit

Use the form below to enter and name your DNA fragments. You may upload the sequences in FASTA or plain text format.

If the adjacent sequences do not contain the required end-terminal homology, check the PCR box below the fragment sequence. PCR primers will then be designed that add the required homology to your fragment when generated by PCR.

  • Select the recommended vector, pUC19L, or enter your own vector.
  • Assemble up to 4 fragments plus a vector.
  • Maximum recommended size of the final assembled construct is 13 kb.
  • Fragments are assembled 5' to 3' in the order entered.
  • Depending on the final construct and individual fragment sizes, fragments may need to be precloned before assembly. Instructions will be provided if precloning is recommended.
  • If PCR amplification is not selected for a vector, primers for the first and last fragments may be long.

Vector

Use recommended vector pUC19L
Import Vector
Sequence
Design primers to PCR amplify for assembly: