clear search
Search Suggestions
Recent searches Clear History
Contact Us

t6 Mountain juniper Pollen

Whole Allergen
Code t6
LOINC 6178-8
Family Cupressaceae
Genus Juniperus
Species Juniperus sabinoides and Juniperus ashei
Route of Exposure Inhalation
Source Material Pollen
Latin Name Juniperus sabinoides and Juniperus ashei
Other Names Mountain cedar, Ashe juniper
Categories Tree Pollens


Mountain juniper, also known as mountain cedar, is an above-average shrub or an undersized tree, which can attain a height of up to 15 m. It is known to have a high pollen-producing capability. The pollination period of this tree falls from December to February. Native to North America, this tree is prevalent in countries like the US and Mexico. Exposure to mountain juniper pollen may induce symptoms such as asthma, allergic rhinitis, allergic rhino-conjunctivitis, and oral allergy syndrome (OAS) in sensitive patients. Jun a 1, Jun a 2, Jun a 3 and Jun a 7 potential allergens have been identified in the IUIS database. Allergen immunotherapy with mountain juniper pollen has been reported to have a significant improvement in patients with allergy symptoms. Mountain juniper has exhibited cross-reactivity with related species like Arizona cypress, Japanese cypress, Port Orford cedar, red cedar, western red cedar, western juniper, Pfitzer juniper etc. and with unrelated species like peach, tomato, apple, kiwi etc.



Mountain juniper or mountain cedar is known by the scientific Latin names Juniperus sabinoides and Juniperus ashei (J. ashei) and belongs to the Cupressaceae family (1). It is a dioecious, small-sized tree or a large-sized shrub that can grow up to 35-40 feet in height (2, 3). The tree has an open, irregular, column or pyramid-shaped crown and a spread of 15 to 25 feet (3) with grey to reddish-brown mature barks (2, 3). The leaves are green, scaled, triangle to oval-shaped with < 2 inches in length. It produces seeds of 2 mm in size (length) with a thick wood-like outer coating (2, 3).

 The species from the Cupressaceae family are capable of producing pollens in huge quantities, approximately 0.4 million pollen grains per male cone and 276 thousand million pollen grains per tree (4). The juniper pollen dispersion generally occurs during mild temperature conditions and avoids cold conditions (5). The pollination period of J. ashei usually occurs from December to March (winters) with high frequency and production quantity. The pollens may be airborne and transported over long distances, especially during the winter (4, 6). This tree is utilized mainly as an ornamental planting in the United States (US) as well as other countries (7, 8). 


Juniperus species are observed to grow in natural areas and unmaintained farmlands (4). Mountain juniper tree is mainly prevalent in high altitude regions (8). They preferably grow on superficial calcareous soil in the eastern US to sharp hilly slopes of low to mid-level altitudes in the western US (9). It prefers bright sunlight, dry climatic region with negligible humidity for growth and is able to grow in well-drained soil with a varied composition like clay, loamy and sandy. This tree is drought tolerant and able to sustain in acidic or alkaline pH soil (3).

The pollen dispersal of J. ashei generally requires conditions like dry weather with mild temperatures (above 5 ºC) and <50% relative humidity persisting for 24 hours (10).


Cupressaceae is a conifer family that is categorized into seven different subfamilies, 30 genera and close to 160 species. Mountain juniper is a tree that belongs to the family of Cupressaceae and genus juniper. Of interest, the genus juniper, along with the other four genera, i.e. Chamaecyparis, Hesperocyparis, Cupressus and Cryptomeria are considered to be the most essential taxa for allergic diseases (4). 

Taxonomic tree of  Mountain juniper(1,11)
Domain Eukaryota
Kingdom Plantae
Phylum Spermatophyta
Subphylum Gymnospermae
Class Pinopsida
Order Pinales
Family Cupressaceae
Genus Juniperus
Species Juniperus sabinoides  Juniperus ashei


Pollen from J. ashei (Mountain juniper), member of the Cupressaceae family, are recognized as highly allergenic in North America. Their pollens are not easily distinguishable among the same species by their morphological characteristics. The pollen from this tree is reported to have a size ranging between 19-22 µm (diameter) and a weight of 2.45 – 4.6 ng (12).

To date, Jun a 1, Jun a 2, Jun a 3 and Jun a 7 have been identified from the pollen of J. ashei (Mountain Juniper) and listed by the World Health Organization/International Union of Immunological Studies (WHO/IUIS) Allergen Nomenclature Sub-Committee (13).


Worldwide distribution

Cupressaceae family pollen is recognized as one of the 12 most abundant aeroallergen pollens in Europe (4). Further, Mountain juniper pollen is highly allergenic and is considered as one of the crucial reasons for allergenic rhinitis (hay fever) in Texas and North America (12).

Mountain juniper pollinosis is a widespread clinical allergic condition found in Mexico and Texas, US (7). In contrast, eastern red cedar (Juniperus virginiana) and white cedar (Thuja occidentalis) from the same family Cupressaceae are common allergenic plants in western New York, US (14). Studies have also identified air pollutants as a risk factor for Cupressaceae pollen and its correlation with the allergy arising from the exposure to the pollen of this family (4).

In a study conducted in Texas, 34% (80/234) of the patients were mono-sensitized to mountain juniper, and 66% (154/234) were poly-sensitized to other aeroallergens in addition to mountain juniper. Asthma and atopic dermatitis were the commonly observed allergic reactions among the mono-sensitized and polysensitized group (7).

An Iranian study conducted on 306 patients suffering from allergic rhinitis reported 17.3% of the patients exhibiting positive skin prick test (SPT) towards mountain juniper (15). 

Environmental Characteristics

Worldwide distribution

Mountain juniper tree is reported to be originated from North America (3) and is prevalent in countries like the United States and Mexico (12).

Route of Exposure Section


Pollens from mountain juniper can enter the respiratory tract through inhalation (7).

Clinical Relevance

Mountain juniper pollen allergenicity causes severe seasonal allergic diseases in several parts of the US and northern regions of Mexico. Allergic rhinitis and asthma, occurring due to aeroallergens, particularly mountain juniper, are among the primary reasons for increased morbidity, productivity loss and increased burden on healthcare expenditure. (16). Symptoms like allergic rhinitis and conjunctivitis may cause impairment of sleep, decrease in quality of life and decline in cognitive function in allergic individuals (17).

Allergic rhinitis (AR) and allergic rhino-conjunctivitis (ARC)

In a US-based study on 234 allergic patients, 34% (n=80) reported mono-sensitization to mountain juniper. Over one-third of these mono-sensitized patients depicted the development of allergic rhinitis and conjunctivitis as their only allergic symptoms (7).

Further, in another study in Texas (US), 21 out of 26 patients with a history of ARC due to mountain juniper (mountain cedar) were confirmed to have ARC due to mountain juniper (mountain cedar) based on subcutaneous or intradermal testing (6).


In a US-based study, 11% of patients with mountain juniper mono-sensitization and 39% of patients with poly-sensitization to several aeroallergens, including mountain juniper, had asthma or atopic dermatitis (7). Further, in another study in Texas (US), only 4 patients out of 21 allergic patients due to mountain juniper (mountain cedar) had a history of stable asthma (6).

Other diseases

Oral Syndrome allergy (OAS)

A study on 28 mountain juniper-sensitized individuals with a suspected food allergy reported cross-reactive IgE binding between mountain juniper pollen and fruit allergen. OAS was observed to have manifested in 53.5% (15/28) of these individuals. The individuals with OAS were found positive towards tomato (11 patients), banana (6 patients) and apple (1 patient) based on SPT. Also, patients with OAS showed stronger in-vitro and cutaneous reactivity to mountain juniper pollen. Jun a 2 was reported as one of the potential allergens to induce OAS due to tomato pollen hypersensitivity. Additionally, it was suggested that co-relation between mountain juniper sensitivity and tomato, banana, and apple sensitivity should be considered during diagnosis (18).

Prevention and Therapy

Allergen immunotherapy

A randomized study was conducted in Texas (US) on 21 patients with allergic rhino-conjunctivitis (induced by exposure to mountain juniper pollen) to analyze intra-lymphatic immunotherapy in mountain juniper allergic patients. They received intra-lymphatic injections of allergenic extract containing mountain juniper pollen or placebo every three months prior to mountain juniper pollen season. Patients receiving immunotherapy reported having a significant improvement in allergy symptoms and their medication usage, compared to placebo (p < 0.001) (6).

Prevention strategies


One of the preventive measures to be taken is to cut down the amount of pollen entering the respiratory system. During pollen season, allergic patients are suggested to stay indoors or wear masks outdoors to keep away from pollen allergens. In worse situations, patients may be suggested to move to a non-pollen area (19).

Other measures like removing all diseased trees from surrounding areas as they produce 2 to 3 times more pollens than the healthy ones; trimming windbreaks during autumn season to remove the male cones, can help (20).

One of the important aspects of pollen-related food allergy management is the detection and avoidance of cross-reactive allergy-related food products. Patients may be advised to avoid raw and processed fruits and vegetables from the diet (21). 

Molecular Aspects

Allergenic molecules

Major allergens such as Jun a 1, Jun a 2, Jun a 3 and Jun a 7 have been identified in various immunoblot analyses, which showed IgE binding to these proteins and listed in the WHO/IUIS database (13). 

Allergen Biochemical name Molecular weight Allergenicity
Jun a 1 Pectate lyase 43
  • Previously known as Jun s 1
  • Major allergen (22).
  • IgE reactivity was observed in 71.4% of 14 sera from mountain juniper allergic patients as seen on immunoblot analysis (1).
Jun a 2 Polygalacturonase 43
  • IgE reactivity was observed in all 8 sera of Japanese cypress allergic patients and one mountain juniper allergic patient as seen on immunoblot analysis (23). 
Jun a 3 Thaumatin-like protein 30
  • PR-5 protein (16)
  • IgE reactivity was observed in 42.9% and 33.3% of 14 sera from mountain juniper allergic patients and 36 sera from Japanese cedar sensitive patients, respectively, as seen on ELISA immunoblots (16).
Jun a 7 Gibberellin-regulated protein 7
  • High IgE levels were detected in 16 sera of peach allergic and cypress pollen sensitized individuals (24).

kDa: kilodaltons, IgE: Immunoglobulin E, PR: Pathogenesis-related, ELISA: Enzyme Linked Immunosorbent Assay

Biomarkers of severity

Studies have identified Jun a 1 as the major marker allergen for mountain juniper allergy (22).


Pollen-pollen cross-reactivity

Proteins from the pollen of 12 species of Cupressaceae, including mountain juniper and one Taxodiaceae member Japanese cedar, have exhibited extensive cross-reactivity. Jun a 1, a major allergen of mountain juniper, was responsible for the cross-reactivity with other allergens from Cupressaceae family (40 to 42 kDa), and Japanese cedar (46 kDa). Some of the species reported to be cross-reactive with mountain juniper include Port Orford Cypress (Chamaecyparis lawsonia), red cedar (J. virginiana), Italian cypress (C. sempervirens), Arizona cypress (C. arizonica), one-seed juniper (J. monosperma), redberry juniper (J. pinchotti) and other species of Juniperus (10, 25).

Jun a 1, a major allergen (pectate lyase) of mountain juniper (mountain cedar) was found highly cross-reactive with major allergens of Japanese cypress (Cha o 1), Japanese cedar (Cry j 1), and Arizona cypress (Cup a 1) (16, 20). This cross-reactivity may be attributed to homology (70%-95%) among the amino acid sequences of these proteins since they belong to the same family of tree species (24). A study has also reported high amino acid sequence similarity of 91% between Cup a 1 and Jun a 1 (26).

Further, Jun a 2 is also reported to be highly homologous to Cry j 2 (70.7 %) and Cha o 2 (82.0%), allergens of a Japanese cedar tree (Cryptomeria japonica) and Japanese cypress tree (Chamaecyparis obtuse) pollen respectively (23). Also, Cup s 3 from C. sempervirens species is revealed to have 95% sequence homology and strong cross-reactivity with Jun a 3 (PR-5 protein) (27). In addition, Jun a 7 was found to be 98% homologous to Cup s 7 (Cupressus sempervirens, Italian cypress) (24).

Pollen-food cross-reactivity

Jun a 1, Jun a 2 and Jun a 3 have been observed to have high homology with allergens in fruits. OAS was found in mountain juniper allergic patients due to cross-reactivity with tomato, banana, melon mix and apple. Jun a 2 (Polygalacturonase) was reported to be responsible for cross-reactivity with tomato allergen (Sola 14) (18).

Further, Jun a 3, a thaumatin-like pathogenesis-related protein (PR-5), is closely related to a thaumatin-like protein in bananas (28). Cross-reactivity is expected between Jun a 3 and thaumatin-like proteins in cherry (Pru av 2), apple (Mal d 2), and paprika or bell pepper (Cap a 1) and therefore, sensitization to Jun a 3 may induce symptoms of OAS in mountain juniper sensitized patients (Jun a 3) (29).

Additionally, osmotin (PR-5, thaumatin-like protein) homology has been reported in tomato (Lyc e NP24; 91.87%), kiwi fruit (Act c 2; 75%), cypress (Cup s 3; 61.3%), bell pepper, (Cap a 1; 88.16%), apple (Mal d 2; 40.65%) and mountain juniper (Jun a 3; 63.79%). This sequence homology may cause cross-reactivity between these species (30).

Further, Jun a 7 exhibiting 67% of sequence homology to Pru p 7 (peach) has also been reported in a study (24).

Pollen-Fungal Spores cross-reactivity

Fungi homologous allergens have been reported in allergens from pollen origin such as Jun a 2, Tri a 25 (Wheat), Bet v 7 (Birch), Cry j 2, Pla a 2 (London Plane tree), Hor v 4 (Barley).

This speculates the probability of cross-sensitization between fungi and pollen (31).

Compiled By

Author: Turacoz Healthcare Solutions

Reviewer: Dr. Fabio Iachetti


Last reviewed: January 2021

  1. Midoro-Horiuti T, Goldblum RM, Kurosky A, Goetz DW, Brooks EG. Isolation and characterization of the mountain cedar (Juniperus ashei) pollen major allergen, Jun a 1. J Allergy Clin Immunol. 1999;104(3 Pt 1):608-12.
  2. Smeins F, Fuhlendorf S, editors. Biology and ecology of Ashe juniper. Juniper symposium; 1997: Texas A&M Research and Extension Center.
  3. Gilman EF, Watson DG. Juniperus Ashei (Ashe Juniper). 1993.
  4. Charpin D, Pichot C, Belmonte J, Sutra J-P, Zidkova J, Chanez P, et al. Cypress Pollinosis: from Tree to Clinic. Clinical Reviews in Allergy & Immunology. 2017;56.
  5. Monroy-Colín A, Manzano JMM, Silva-Palacios I, Tormo-Molina R, Pecero-Casimiro R, Gonzalo-Garijo Á, et al. Phenology of Cupressaceae urban infrastructure related to its pollen content and meteorological variables. Aerobiologia. 2020:1-21.
  6. Thompson CP, Silvers S, Shapiro MA. Intralymphatic immunotherapy for mountain cedar pollinosis: A randomized, double-blind, placebo-controlled trial. Annals of Allergy, Asthma & Immunology. 2020;125(3):311-8.e2.
  7. Ramirez DA. The natural history of mountain cedar pollinosis. J Allergy Clin Immunol. 1984;73(1 Pt 1):88-93.
  8. Hrabina M, Dumur J-P, Sicard H, Viatte A, André C. Diagnosis of cypress pollen allergy: in vivo and in vitro standardization of a Juniperus ashei pollen extract. Allergy. 2003;58.
  9. Van Auken O, Jackson J, Jurena P. Survival and growth of Juniperus seedlings in Juniperus woodlands. Plant Ecology. 2005;175(2):245-57.
  10. Bunderson LD, Levetin E. Hygroscopic weight gain of pollen grains from Juniperus species. International Journal of Biometeorology. 2015;59(5):533-40.
  11. CABI. Juniperus ashei (Ashe juniper) 2019 [05.01.2021]. Available from:
  12. Mohanty RP, Buchheim MA, Anderson J, Levetin E. Molecular analysis confirms the long-distance transport of Juniperus ashei pollen. PLoS One. 2017;12(3):e0173465-e.
  13. WHO/IUIS. Juniperus ashei allergens 2020 [cited 2020 25-Nov-2020]. Available from:
  14. Deane PM, editor Conifer pollen sensitivity in western New York: cedar pollens. Allergy and asthma proceedings; 2005: OceanSide Publications.
  15. Fereidouni M, Hossini RF, Azad FJ, Assarezadegan MA, Varasteh A. Skin prick test reactivity to common aeroallergens among allergic rhinitis patients in Iran. Allergologia et immunopathologia. 2009;37(2):73-9.
  16. Midoro-Horiuti T, Goldblum RM, Kurosky A, Wood TG, Brooks EG. Variable expression of pathogenesis-related protein allergen in mountain cedar (Juniperus ashei) pollen. J Immunol. 2000;164(4):2188-92.
  17. Basak P, Arayata R, Brensilver J. Prevalence of specific aeroallergen sensitivity on skin prick test in patients with allergic rhinitis in Westchester County. The Internet Journal of Asthma, Allergy and Immunology. 2008;6(2).
  18. Bonds R, Sharma GS, Kondo Y, van Bavel J, Goldblum RM, Midoro-Horiuti T. Pollen food allergy syndrome to tomato in mountain cedar pollen hypersensitivity. Molecular immunology. 2019;111:83-6.
  19. Xie Z-J, Guan K, Yin J. Advances in the clinical and mechanism research of pollen induced seasonal allergic Asthma. American journal of clinical and experimental immunology. 2019;8(1):1.
  20. Charpin D, Calleja M, Lahoz C, Pichot C, Waisel Y. Allergy to cypress pollen. Allergy. 2005;60(3):293-301.
  21. Muluk NB, Cingi C. Oral allergy syndrome. American journal of rhinology & allergy. 2018;32(1):27-30.
  22. Wallner M, Ferreira F, Hofer H, Hauser M, Mahler V, Kleine-Tebbe J. The Concept of Pollen Panallergens: Profilins and Polcalcins. 2017. p. 43-56.
  23. Yokoyama M, Miyahara M, Shimizu K, Kino K, Tsunoo H. Purification, identification, and cDNA cloning of Jun a 2, the second major allergen of mountain cedar pollen. Biochem Biophys Res Commun. 2000;275(1):195-202.
  24. Ehrenberg AE, Klingebiel C, Östling J, Larsson H, Mattsson L, Vitte J, et al. Characterization of a 7 kDa pollen allergen belonging to the gibberellin‐regulated protein family from three Cupressaceae species. Clinical & Experimental Allergy. 2020.
  25. Schwietz LA, Goetz DW, Whisman BA, Reid MJ. Cross-reactivity among conifer pollens. Ann Allergy Asthma Immunol. 2000;84(1):87-93.
  26. Aceituno E, Del Pozo V, Minguez A, Arrieta I, Cortegano I, Cardaba B, et al. Molecular cloning of major allergen from Cupressus arizonica pollen: Cup a 1. Clinical & Experimental Allergy. 2000;30(12):1750-8.
  27. Togawa A, Panzani R, Garza M, Goldblum R, Midoro-Horiuti T. Isolation and characterization of the major allergen from Italian cypress (Cupressus sempervirens) pollen. Journal of Allergy and Clinical Immunology. 2004;113(2):S300.
  28. Ghosh R, Chakrabarti C. Crystal structure analysis of NP24-I: a thaumatin-like protein. Planta. 2008;228(5):883.
  29. Midoro-Horiuti T, Brooks EG, Goldblum RM. Pathogenesis-related proteins of plants as allergens. Annals of allergy, asthma & immunology. 2001;87(4):261-71.
  30. Sharma P, Singh AK, Singh BP, Gaur SN, Arora N. Allergenicity assessment of osmotin, a pathogenesis-related protein, used for transgenic crops. Journal of agricultural and food chemistry. 2011;59(18):9990-5.
  31. Nasser SM, Pulimood TB. Allergens and thunderstorm asthma. Curr Allergy Asthma Rep. 2009;9(5):384-90.