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          • Proteins & Peptides ›
          • EGFR (T790M, C797S, L858R Mutant) Proteins

          Invitrogen

          Human EGFR (ErbB1) [T790M, C797S, L858R], GST Tag Recombinant Protein

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          Cite Human EGFR (ErbB1) [T790M, C797S, L858R], GST Tag Recombinant Protein

          Product Details

          A33725

          Applications
          Tested Dilution
          Publications

          Kinase Assay (KA)

          Assay-dependent
          -
          Product Specifications

          Species

          Human

          Expression System

          Insect cells

          Amino acid sequence

          695-end

          Tag

          GST-tag

          Molecular weight

          84.2 kDa

          Class

          Recombinant

          Type

          Protein

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          See Label

          Purification

          purified

          Storage buffer

          50mM tris HCl, pH 7.5, with 150mM NaCl, 0.1mM PMSF, 0.1mM EDTA, 25% glycerol, 0.25mM DTT, 10mM glutathione

          Contains

          no preservative

          Storage conditions

          -80°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Dry ice

          Product Specific Information

          For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

          This EGFR (ErbB1) T790M C797S L858R recombinant human protein (695–end) was expressed in insect cells.



          • Validated for use with the Z’-LYTE™ assay
          • Available in multiple pack sizes for convenient use
          • Also available in our SelectScreen Kinase Profiling Service



          Protein Form: Recombinant, Active, Cytoplasmic, Mutant

          Target Information

          EGFR receptor tyrosine kinase is a transmembrane glycoprotein. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival. Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms. Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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