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Invitrogen
Human Glucocorticoid Receptor, Ligand Binding Domain, GST Tag Recombinant Protein
Product Details
A15668
Product Specifications
Species
Human
Expression System
Baculovirus
Amino acid sequence
521-777
Tag
GST-tag
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
See Label
Purification
purified
Storage buffer
proprietary buffer
Contains
no preservative
Storage conditions
-80° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
Glucocorticoid receptor ligand binding domain (GR-LBD) is a 57.85 kDa recombinant human protein (amino acids 521-777) expressed as a glutathione-S-transferast (GST) fusion protein in baculovirus-infected insect cells. GR-LBD is expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator displacement studies.
GR-LBD human recombinant protein is:
• Flexible—use in your in-house assay or as part of our LanthaScreen™ Coactivator or Competitive Binding assays for a complete assay solution
• Ready to use—active and tagged (with GST)
• Convenient—available in bulk sizes
Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.
Protein Form: Ligand Binding Domain, Recombinant
Target Information
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858
Gene Aliases: GCCR; GCR; GCRST; GR; GRL; NR3C1
UniProt ID: (Human) P04150
Entrez Gene ID: (Human) 2908
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