Proteomics Liquid Chromatography (LC) Separations

Addressing the complexity of proteomics

A proteome is a complex system spanning several orders of magnitude in terms of dynamic range. For successful characterization, peptides and proteins must be separated prior to introduction into the mass spectrometer. Minimizing complexity ensures that peptides and proteins can be detected and sequenced even if they are present at low abundance. Our products deliver comprehensive breadth for proteomics analysis, with liquid chromatography (LC) systems, columns, and chemistries. When combined with leading Orbitrap mass spectrometry (MS) systems, our solutions enable broader and deeper analyses into the proteome.

Featured proteomics categories

Minimize protein complexity

Sample complexity must be minimized if protein analysis by MS is to be successful. Proteins must be well-resolved from one another and from various proteoforms to minimize the low thresholds of protein detection. Thermo Scientific ProSwift RP-4H LC Columns are ideal for protein separation prior to MS analysis. They are also designed to work with milliliter to microliter flow.

Find high performance and simplicity

Reversed-phase liquid chromatography (RP-LC) is the gold standard for separation of peptides and offers the broadest range of peptide coverage. Reversed-phase separation on Thermo Scientific low-flow systems is an ideal part of an integrated proteomics workflow; it also seamlessly combines with Thermo Scientific Orbitrap mass spectrometers. The Thermo Scientific EASY-nLC 1200 System offers operational simplicity and high performance.

Achieve the highest separation

Designed for ultra high-resolution separation of proteins, the Thermo Scientific UltiMate 3000 Rapid Separation systems, including the Thermo Scientific UltiMate 3000 RSLCnano System with Thermo Scientific ProFlow technology, enable milliliter to nanoliter flow separations and easy integration with mass spectrometry analyzers, providing excellent resolution, sensitivity, and selectivity for protein applications.