Hydrogen Deuterium Exchange (HDX)
Robust method for full structural characterization
Hydrogen deuterium exchange (HDX) mass spectrometry (MS) is a powerful tool for studying the dynamics of higher order structure of protein-based therapeutics. The rate of hydrogen-to-deuterium exchange within the amide hydrogen on the backbone of biotherapeutics provides solvent accessibility information and thus protein structure and conformation can be inferred. Orbitrap-based HDX MS using the Thermo Scientific Orbitrap Fusion Lumos Tribrid MS offers a robust method for the analysis of protein conformation, conformation dynamics, and protein-protein interactions.
Full structural characterization is critical in biopharmaceutical development where the subtle but critical local conformational changes can impact safety and efficacy.
Hydrogen deuterium exchange (HDX) workflow
Test new limits of detection, characterization, and quantitation using the Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer. This latest Tribrid MS was designed to expand performance in advanced biopharma. The instrument features enhanced sensitivity resulting in improved analyte detection, characterization, and quantitation, enabling scientists to perform more comprehensive sample analyses faster and with better accuracy than ever before.
Save time and identify more with Thermo Scientific BioPharma Finder integrated software. It provides an automated workflow for peptide identification, disulfide bond mapping, and quantitation of post-translational modifications (PTMs). BioPharma Finder software automates previously time-consuming manual processes, processes complex data, and integrates the results into concise, informative reports.
Simplify a wide range of workflows—from protein and peptide identification to PTM analysis—using Thermo Scientific Proteome Discoverer software. The software supports multiple database search algorithms (SEQUEST, Z-Core, Mascot, etc.) and multiple dissociation techniques (CID, HCD, ETD) for more comprehensive analyses.