| Fastest live assay | Most widely used live assay | Economical live assay | Most economical | Water-soluble alternative to MTT | |
|---|---|---|---|---|---|
| Plate-based Product Comparison | PrestoBlue | alamarBlue | Resazurin Powder | MTT Powder | XTT powder |
| Assay Type | Resazurin based | Resazurin based | Resazurin based | Tetrazolium based | Tetrazolium based |
| Workflow | Single addition | Single addition | Weigh powder, prepare solution + single addition | Weigh powder, prepare solutions + two additions | Weigh powder, prepare solutions + two additions |
| Incubation time | > = 10 minutes | 1-4 hours | 4 hrs | >= 2 hours | 2-4 hours |
| Detection Method | Absorbance or Fluorescence | Absorbance or Fluorescence | Absorbance or Fluorescence | Absorbance | Absorbance |
| Cell Format | Live | Live | Live | Lysis/Endpoint | Lysis/Endpoint |
| Compatible with Phenol Red |  |
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| Sensitivity | 12 cells/well | 50 cells/well | 100 cells/well | 1,000 cells/well | 1,000 cells/well |
| Instrument | Spectro- photometer, Microplate Reader | Spectro- photometer, Microplate Reader | Spectro- photometer, Microplate Reader | Spectro- photometer, Microplate Reader | Spectro- photometer, Microplate Reader |
| Price per 96-well plate | $$ | $$ | $ | $ | $$ |
| Order now | Order now | Order now | Order now | Order now |
High-sensitivity fluorescence detection for 96-1,536 samples can be quickly performed on Fluoroskan or Fluoroskan FL Microplate Fluorometer or Varioskan LUX Multimode Microplate Reader using Invitrogen reagents for optimal detection. Take advantage of automatic dynamic range selection to get optimal gain settings for each individual well and automation capabilities for even higher throughput.
For Research Use Only. Not for use in diagnostic procedures.
