PCR Fidelity Calculator
Example
What percent of the product molecules contain an error after PCR (30 cycles) with different polymerases?
Polymerase |
1 kb template |
3 kb template |
---|---|---|
Phusion High-Fidelity DNA Polymerases (HF Buffer) |
1.32% | 3.96% |
Phusion High-Fidelity DNA Polymerases (GC Buffer) |
2.85% |
8.55% |
Pyrococcus furiosus DNA polymerase |
8.4% |
25.2% |
Taq DNA polymerase |
68.4% |
205.2% |
The table above demonstrates the low error rate of Phusion DNA Polymerase. After 30 cycles of PCR amplifying a 3 kb template, only 3.96 % of the product DNA molecules contain 1 (nucleotide) error each. This means that 96.04 % of the product molecules are entirely error-free. In contrast, after the same PCR protocol performed with Taq DNA polymerase, every product molecule contains an average of 2 errors.
For Research Use Only. Not for use in diagnostic procedures.