Amniotic Fluid and Chorionic Villus Cells
AmnioMAX™ C-100 medium was developed specifically for the in vitro diagnostic testing of human amniotic fluid specimens and chorionic villus sampling. Designed to help minimize diagnostic turnaround time, AmnioMAX™ medium maximizes colony growth while maintaining the cytogenetic analyzability of resulting colonies.
AmnioMAX™-II Complete Medium is a second-generation formulation, further optimized for cell attachment and increased pH stability. Supplied in a single bottle, it is complete with antibiotics and growth promoting agents, and requires no further supplementation.
AmnioMAX™ medium supports the growth of amniocytes (Figure 1) and delivers superior plating density. These results demonstrate that human amniocytes cultured in AmnioMAX™ medium give rise to approximately 22% to 25% more colonies and 44% to 80% more cytogenetically analyzable colonies as compared to other amniocyte media. In addition, as shown in Figure 2, the mean mitotic index rate of cultured primary amniocytes grown in AmnioMAX™ medium was significantly greater than that achieved with Chang medium.
|Figure 1 - Growth kinetics of aminocytes cultured with AmnioMAX™ medium. A representative growth curve of secondary aminocytes cultured in either AminoMAX™ medium or MEM-alpha supplemented with 20% FBS is shown. Cell counts were taken at various intervals. Results shown are the mean of duplicate samples.|
|Figure 2 - Mitotic rate comparison of AmnioMAX™ medium and Chang medium. The relative mitotic index rate for primary aminocytes cultured in either AminoMAX™ medium or Chang medium in situ for 6 days was determined. The number of mitotic events per 5,000 cells was scored for each culture medium.|