Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein G is a recombinant protein expressed in Escherichia coli (21.6 kDa; 2 IgG binding sites) and is recommended for purifying IgG from mouse, human, cow, goat, or sheep serum. The POROS™ MabCapture™ G Select Resin is a new product that can be scaled up for bioprocess applications.

We offer a wide selection of base supports and formats to purify antibodies using the Protein G ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.

  • Multiple formats—magnetic beads, loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scales
  • High performance—resins are designed to maximize protein yield and reduce background
  • Economical—pricing is similar to or better than our leading competitors

Choose the right Protein G product for antibody purification

Product links in the last row of each column

  Dynabeads Protein G Magnetic Beads Pierce Protein G Agarose Pierce Protein G Plus Agarose POROS MabCapture G Select
Bead or resin size 2.8 μm 45–165 μm 45–165 μm 50 μm
Binding capacity 25–30 µg human IgG/mg beads 11–15 mg human IgG/mL resin ≥20 mg human IgG/mL resin ≥13 mg/mL human IgG/mL resin
Maximum linear flow rate N/A 700 cm/hr 700 cm/hr 3000 cm/hr
Support Monosized, super paramagnetic polystyrene beads Crosslinked 6% beaded agarose Crosslinked 6% beaded agarose Polystyrene-divinylbenzene
Recommended application scale screening batch, pilot batch, pilot batch, pilot, process
Formats available loose beads loose beads, pre-packed spin columns and kits, chromatography cartridges, spin plates loose beads loose beads
Order product(s) 10003D 20398
(loose resin)
(1 mL cartridge)
(5 mL cartridge)
(spin plates)
(loose resin)

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