T Cell Activation and Expansion
Gibco Dynabeads magnetic beads are the fastest growing method for T cell activation for many reasons:
- Physiological activation
- Highly consistent activation and expansion
- No need for autologous APC's, mitogens, antigens, or feeder cells
- Cytokine profile and a full TCR repertoire is retained
- Available for both human and mouse T cells
- Method translates from mouse studies to human immunotherapy on the same platform
Fastest growing method for T cell activation
From the graph, we can see that methods such as those listed below are not used significantly more in 2016 than in 2010:
- Generic mitogen-based T cell activation (home-brew),
- Soluble and/or plate-bound antibodies,
- Antigen presenting cells (APC’s)
However, during this same period, publications citing the use of Dynabeads magnetic beads have increased dramatically.
Dynabeads T-Activator CD3/CD28 products for human and mouse are ideal for short time stimulation studies and T cell expansion of polyclonal T cells (e.g T cell subsets isolated from whole blood, MNC or tissue digests).
"I used the CD3/CD28 Dynabeads for my proliferation experiment, and the experiment worked beautifully! I have been trying to get to work for a couple of months using soluble CD28/CD3, and it was just not happening. Dynabeads have definitely made my day!"
Lisa S. Thomas, Research Associate IV, Cedars-Sinai Medical Center
Key Benefits
- Ease-of-use: No need to prepare autologous APC, feeder cells, or antigen. No need to pre-coat antibodies to beads.
- Physiological activation: Simultaneous signals with agonistic monoclonal antibodies to CD3/TCR and CD28.
- Stimulate and re-stimulate: Release and remove the magnetic beads. Cells can be re-stimulated several times.
- Reliability: Cells keep their functionality with a cytokine profile and a full T cell repertoire reflecting that of in vivo settings.
Main applications
The Dynabeads technology allows you to activate and/or expand naïve and memory T cells, T cell clones, regulatory T cells, NKT cells and CTLs from mouse and human samples.
The technology allows for the activation of T cells for signaling experiments (proteomics), T cell expansion, T helper cell differentiation (Th1, Th2, Th17), expansion of regulatory T cells and T cell activation for improved transduction efficiency of MNC/T cells. A clinical research grade version is also available, allowing you to move from mouse studies to clinical research using the same technology platform.
The main application for these products is T cell re-stimulation and expansion of antigen-specific (Ag-specific) T cells from cell lines, cell clones or freshly isolated/enriched Ag-specific T cells.
Key benefits:
- Physiological activation: Stimulation of CD3/TCR, CD28 and CD137 with agonistic monoclonal antibodies
- Efficiency: Increased expansion and survival of Ag-specific T cells
- Reliability: T cells expanded with Dynabeads maintain Ag-specificity when tested in bioassays
- Ease- of-use: No need to find or prepare donor matched APCs or antigen
To allow for expansion of human antigen-specific T cells, the Dynabeads are conjugated with anti-CD3, anti-CD28, and anti-CD137 antibodies. CD137 (4-1BB) is a member of the tumor necrosis factor family and agonistic anti-CD137 antibodies act as an activating co-stimulatory molecule especially important for effector/ memory T cells and promote the survival and proliferation of T lymphocytes.
This technology allows you to activate and/or expand naïve and memory T cells, T cell clones, regulatory T cells, NKT cells and CTLs from mouse and human samples. A clinical research grade product is also available, allowing you to move from mouse studies to clinical research using the same technology platform.
The new Dynabeads T-Activator CD3/CD28/CD137 for expansion of human antigen-specific T cells are conjugated with anti-CD3, anti-CD28, and anti-CD137 antibodies. CD137 (4-1BB) is a member of the tumor necrosis factor family and agonistic anti-CD137 antibodies act as an activating co-stimulatory molecule especially important for effector/ memory T cells and promote the survival and proliferation of T lymphocytes.
The Dynabeads technology allows you to activate and/or expand naïve and memory T cells, T cell clones, regulatory T cells, NKT cells and CTLs from mouse and human samples.
Our full portfolio of products also include a clinical research grade version, allowing you to move from mouse studies to clinical research using the same technology platform.
Dynabeads T-Activator CD3/CD28 products for human and mouse are ideal for short time stimulation studies and T cell expansion of polyclonal T cells (e.g T cell subsets isolated from whole blood, MNC or tissue digests).
"I used the CD3/CD28 Dynabeads for my proliferation experiment, and the experiment worked beautifully! I have been trying to get to work for a couple of months using soluble CD28/CD3, and it was just not happening. Dynabeads have definitely made my day!"
Lisa S. Thomas, Research Associate IV, Cedars-Sinai Medical Center
Key Benefits
- Ease-of-use: No need to prepare autologous APC, feeder cells, or antigen. No need to pre-coat antibodies to beads.
- Physiological activation: Simultaneous signals with agonistic monoclonal antibodies to CD3/TCR and CD28.
- Stimulate and re-stimulate: Release and remove the magnetic beads. Cells can be re-stimulated several times.
- Reliability: Cells keep their functionality with a cytokine profile and a full T cell repertoire reflecting that of in vivo settings.
Main applications
The Dynabeads technology allows you to activate and/or expand naïve and memory T cells, T cell clones, regulatory T cells, NKT cells and CTLs from mouse and human samples.
The technology allows for the activation of T cells for signaling experiments (proteomics), T cell expansion, T helper cell differentiation (Th1, Th2, Th17), expansion of regulatory T cells and T cell activation for improved transduction efficiency of MNC/T cells. A clinical research grade version is also available, allowing you to move from mouse studies to clinical research using the same technology platform.
The main application for these products is T cell re-stimulation and expansion of antigen-specific (Ag-specific) T cells from cell lines, cell clones or freshly isolated/enriched Ag-specific T cells.
Key benefits:
- Physiological activation: Stimulation of CD3/TCR, CD28 and CD137 with agonistic monoclonal antibodies
- Efficiency: Increased expansion and survival of Ag-specific T cells
- Reliability: T cells expanded with Dynabeads maintain Ag-specificity when tested in bioassays
- Ease- of-use: No need to find or prepare donor matched APCs or antigen
To allow for expansion of human antigen-specific T cells, the Dynabeads are conjugated with anti-CD3, anti-CD28, and anti-CD137 antibodies. CD137 (4-1BB) is a member of the tumor necrosis factor family and agonistic anti-CD137 antibodies act as an activating co-stimulatory molecule especially important for effector/ memory T cells and promote the survival and proliferation of T lymphocytes.
This technology allows you to activate and/or expand naïve and memory T cells, T cell clones, regulatory T cells, NKT cells and CTLs from mouse and human samples. A clinical research grade product is also available, allowing you to move from mouse studies to clinical research using the same technology platform.
The new Dynabeads T-Activator CD3/CD28/CD137 for expansion of human antigen-specific T cells are conjugated with anti-CD3, anti-CD28, and anti-CD137 antibodies. CD137 (4-1BB) is a member of the tumor necrosis factor family and agonistic anti-CD137 antibodies act as an activating co-stimulatory molecule especially important for effector/ memory T cells and promote the survival and proliferation of T lymphocytes.
The Dynabeads technology allows you to activate and/or expand naïve and memory T cells, T cell clones, regulatory T cells, NKT cells and CTLs from mouse and human samples.
Our full portfolio of products also include a clinical research grade version, allowing you to move from mouse studies to clinical research using the same technology platform.
