Phospholipidosis and steatosis are toxic side effects of lipid metabolism that can be triggered by drugs or other compounds. Phospholipidosis is a lysosomal storage disorder associated with cationic amphiphilic drugs and characterized by the accumulation of excess phospholipid complexes within the internal lysosomal membranes. Steatosis is the abnormal retention of lipids within a cell due to impairment of the normal processes of synthesis and elimination of triglyceride fats.
| HCS LipidTOX™ neutral lipid stains were developed for image-based high-content screening (HCS) assays to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ assay series includes neutral lipid stains for fixed cells and phospholipid stains for live cells, which can be combined in multiplexed assays.
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 Lipid vesicles in bone marrow—derived hMSCs visualized with LipidTOX™ Green neutral lipid stain and counterstained with Hoechst 33342. |
| Readout | High affinity for neutral lipid droplets in fixed cells | ||
| Common filter set | Cy®5 | Texas Red® | FITC |
| Reporter | LipidTOX™ Deep Red neutral lipid stain | LipidTOX™ Red neutral lipid stain | LipidTOX™ Green neutral lipid stain |
| Ex/Em (nm) | 637/655 | 577/609 | 495/505 |
| Live-cell compatible | No | ||
| Added to growth media | No | ||
| Formaldehyde fixable | Cells labeled after fixation | ||
| Multiplexing | Can be multiplexed with phospholipidosis detection reagents (see tables below) |
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| Platform | Imaging, HCS | ||
| Bibliography | Citations | ||
| Format | 125 μL (1,200 assays for steatosis/240 assays for adipogenesis) | ||
| Cat. No. | H34477 | H34476 | H34475 |
| Readout | Kit for the sequential analysis of phospholipidosis and steatosis | |
| Common filter set | Texas Red® | FITC |
| Reporter | LipidTOX™ Red phospholipid stain | LipidTOX™ Green neutral lipid stain |
| Ex/Em (nm) | 595/615 | 495/505 |
| Live-cell compatible | Yes | No |
| Added to growth media | Yes | No |
| Formaldehyde fixable | Yes | Labeled after fixation |
| Multiplexing | Combination assay kit for HCS | |
| Bibliography | Citations | |
| Platform | Imaging, HCS | |
| Format | 2 plates/240 assays | 10 plates/1,200 assays |
| Cat. No. | H34157 | H34158 |
| Readout | Labels phospholipid accumulation after incubation with live cells | |
| Common filter set | FITC | Texas Red® |
| Reporter | LipidTOX™ Green phospholipid stain | LipidTOX™ Red phospholipid stain |
| Ex/Em (nm) | 495/525 | 595/615 |
| Live-cell compatible | Yes | |
| Added to growth media | Yes | |
| Formaldehyde fixable | Yes | |
| Multiplexing | Can be multiplexed with neutral lipid stains (see table above) | |
| Bibliography | Citations | |
| Platform | Imaging, HCS | |
| Format | 125 μL (1,200 assays) | |
| Cat. No. | H34350 | H34351 |
| Readout | High affinity for neutral lipid droplets in fixed cells | ||
| Common filter set | Cy®5 | Texas Red® | FITC |
| Reporter | LipidTOX™ Deep Red neutral lipid stain | LipidTOX™ Red neutral lipid stain | LipidTOX™ Green neutral lipid stain |
| Ex/Em (nm) | 637/655 | 577/609 | 495/505 |
| Live-cell compatible | No | ||
| Added to growth media | No | ||
| Formaldehyde fixable | Cells labeled after fixation | ||
| Multiplexing | Can be multiplexed with phospholipidosis detection reagents (see tables below) |
||
| Platform | Imaging, HCS | ||
| Bibliography | Citations | ||
| Format | 125 μL (1,200 assays for steatosis/240 assays for adipogenesis) | ||
| Cat. No. | H34477 | H34476 | H34475 |
| Readout | Kit for the sequential analysis of phospholipidosis and steatosis | |
| Common filter set | Texas Red® | FITC |
| Reporter | LipidTOX™ Red phospholipid stain | LipidTOX™ Green neutral lipid stain |
| Ex/Em (nm) | 595/615 | 495/505 |
| Live-cell compatible | Yes | No |
| Added to growth media | Yes | No |
| Formaldehyde fixable | Yes | Labeled after fixation |
| Multiplexing | Combination assay kit for HCS | |
| Bibliography | Citations | |
| Platform | Imaging, HCS | |
| Format | 2 plates/240 assays | 10 plates/1,200 assays |
| Cat. No. | H34157 | H34158 |
| Readout | Labels phospholipid accumulation after incubation with live cells | |
| Common filter set | FITC | Texas Red® |
| Reporter | LipidTOX™ Green phospholipid stain | LipidTOX™ Red phospholipid stain |
| Ex/Em (nm) | 495/525 | 595/615 |
| Live-cell compatible | Yes | |
| Added to growth media | Yes | |
| Formaldehyde fixable | Yes | |
| Multiplexing | Can be multiplexed with neutral lipid stains (see table above) | |
| Bibliography | Citations | |
| Platform | Imaging, HCS | |
| Format | 125 μL (1,200 assays) | |
| Cat. No. | H34350 | H34351 |
Resources
| Molecular Probes® Handbook |
BioProbes® articles |