kingfisher-flex-large

The Thermo Scientific KingFisher Flex purification system provides plate-based handling (mix, capture, transfer, release) of magnetic particles for bead-based nucleic acid and protein affinity purification procedures. This easy-to-use instrument provides excellent reproducibility and minimizes the hands-on time required for DNA, RNA, and protein isolation from a wide range of sample types. The system is available with four different user interchangeable magnetic head formats, enabling scientists to process a variety of volumes with compatible purification reagents, such as MagMAX nucleic acid extraction products.

Request a demo

Features of the KingFisher Flex include:

  • Easy installation (ready to run in 10 minutes or less)
  • User-friendly graphical interface
  • High-throughput purification of 96 samples per run
  • Four different magnetic head formats for wide volume range (20–5,000 µL)
  • Pre-loaded programs to perform protocols with MagMAX nucleic acid isolation kits
  • Thermo Scientific BindIt Software, which allows programmable instrument control and protocol modification, creation, and upload (Windows 7 and 8 compatible)

Video: How KingFisher Flex works

How it works

The KingFisher Flex magnetic particle processor utilizes permanent magnetic rods and disposable tip combs with independent movement control. When the magnetic rod—sheathed inside the tip comb—is lowered into the solution, magnetic beads collect at the bottom of the tip comb. The tip comb can then be positioned in a different well or plate, and the beads are then released by moving the magnetic rods out of the tip comb. The tip comb also facilitates the mixing of reagents and beads as the magnetic head moves up and down.

KingFisher - How it works

MagMAX kits and the KingFisher Flex system

Magmax kits

When MagMAX kits are used on the KingFisher Flex system, labs will have a simple, fast system that is designed to provide pure nucleic acids ready for downstream applications such as real-time PCR and next-generation sequencing.

Top benefits of running the MagMAX kits on the KingFisher Flex system with 24 Deep-Well Head:

  • Nucleic acid extraction from larger sample input volumes
  • Consistent, high-quality nucleic acid recovery from a wide variety of sample types
  • Cross-contamination control, thanks to bead transfer technology

Choose the right MagMAX kits for your needs

Please visit the product page to obtain the required MagMAX program for your your specific kit or email techsupport@thermofisher.com.

Dynabeads immunoprecipitation

Dynabeads immunoprecipitation

Dynabeads products pioneered biomagnetic technology and ensure that you get the best balance of high yield and reproducibility with low nonspecific binding and cost. This balance is one reason why Dynabeads products have become the gold standard for immunoprecipitation using magnetic beads  and are well suited for automation. Here are many other reasons:

  • Low background—little to no nonspecific binding, and no preclearing and high signal to noise ratio
  • Highly reproducible—uniform beads help ensure consistent results
  • Flexible—products for IP, Co-IP, pull-down, and ChIP assays; ideal for manual and automated protocols
  • Antibody savings—all binding occurs on the smooth outer surface of the beads, which conserves precious antibodies and supports a cost-efficient solution per sample
  • Highly sensitive—Dynabeads technology is the most-cited method for sensitive applications, such as ChIP and IP of low-abundance proteins

Learn more about solutions for immunoprecipitation
Get the newest protocols for automated IP on the KingFisher platform

Plastics for the KingFisher Flex system

Documents

Manuals

Application notes

Technical notes

Posters

Smart note

KingFisher Flex how-to videos

Remove transportation locks

After receiving your KingFisher Flex, this video will walk through the steps to remove the transportation locks.

Install tip shield

Walking through the process to install the tip shield on the KingFisher Flex.

Power on

What to know in order to power on the KingFisher Flex Instrument.

Change heat block

Helping identify the process to change the heat block on the KingFisher Flex instrument.

Install magnet head and check tip comb

Helping with the steps to install the magnet head of the KingFisher Flex and also how to check the tip comb before loading instrument.

Connect computer and use BindIt software

Walking through the process to connect the KingFisher Flex top laptop and installing the BindIt software.

Transfer BindIt software protocols

Update your instrument with your protocol, simple steps to transfer your BindIt protocol to the KingFisher Flex instrument.

Remove magnet head

Simple steps to help remove the magnet head safely from the KingFisher Flex instrument.

How it works

The KingFisher Flex magnetic particle processor utilizes permanent magnetic rods and disposable tip combs with independent movement control. When the magnetic rod—sheathed inside the tip comb—is lowered into the solution, magnetic beads collect at the bottom of the tip comb. The tip comb can then be positioned in a different well or plate, and the beads are then released by moving the magnetic rods out of the tip comb. The tip comb also facilitates the mixing of reagents and beads as the magnetic head moves up and down.

KingFisher - How it works

MagMAX kits and the KingFisher Flex system

Magmax kits

When MagMAX kits are used on the KingFisher Flex system, labs will have a simple, fast system that is designed to provide pure nucleic acids ready for downstream applications such as real-time PCR and next-generation sequencing.

Top benefits of running the MagMAX kits on the KingFisher Flex system with 24 Deep-Well Head:

  • Nucleic acid extraction from larger sample input volumes
  • Consistent, high-quality nucleic acid recovery from a wide variety of sample types
  • Cross-contamination control, thanks to bead transfer technology

Choose the right MagMAX kits for your needs

Please visit the product page to obtain the required MagMAX program for your your specific kit or email techsupport@thermofisher.com.

Dynabeads immunoprecipitation

Dynabeads immunoprecipitation

Dynabeads products pioneered biomagnetic technology and ensure that you get the best balance of high yield and reproducibility with low nonspecific binding and cost. This balance is one reason why Dynabeads products have become the gold standard for immunoprecipitation using magnetic beads  and are well suited for automation. Here are many other reasons:

  • Low background—little to no nonspecific binding, and no preclearing and high signal to noise ratio
  • Highly reproducible—uniform beads help ensure consistent results
  • Flexible—products for IP, Co-IP, pull-down, and ChIP assays; ideal for manual and automated protocols
  • Antibody savings—all binding occurs on the smooth outer surface of the beads, which conserves precious antibodies and supports a cost-efficient solution per sample
  • Highly sensitive—Dynabeads technology is the most-cited method for sensitive applications, such as ChIP and IP of low-abundance proteins

Learn more about solutions for immunoprecipitation
Get the newest protocols for automated IP on the KingFisher platform

Plastics for the KingFisher Flex system

Documents

Manuals

Application notes

Technical notes

Posters

Smart note

KingFisher Flex how-to videos

Remove transportation locks

After receiving your KingFisher Flex, this video will walk through the steps to remove the transportation locks.

Install tip shield

Walking through the process to install the tip shield on the KingFisher Flex.

Power on

What to know in order to power on the KingFisher Flex Instrument.

Change heat block

Helping identify the process to change the heat block on the KingFisher Flex instrument.

Install magnet head and check tip comb

Helping with the steps to install the magnet head of the KingFisher Flex and also how to check the tip comb before loading instrument.

Connect computer and use BindIt software

Walking through the process to connect the KingFisher Flex top laptop and installing the BindIt software.

Transfer BindIt software protocols

Update your instrument with your protocol, simple steps to transfer your BindIt protocol to the KingFisher Flex instrument.

Remove magnet head

Simple steps to help remove the magnet head safely from the KingFisher Flex instrument.