Wash Buffers and Detergents for Western Blotting
Ready-to-use, pre-formulated buffers for Western blotting; includes PBS, PBS-T, TBS, TBS-T (Tween-20). Choose from dry blend packs or pouches of pre-blended powder mixtures of commonly needed buffers such as PBS and TBS for Western blot methods; simply add water to dissolve and prepare or 10X and 20X concentrated solutions of frequently used Western blot buffers; simply dilute to 1X with water and they're ready to use.
Wash buffer & detergent selection guide
TBS | PBS | TBST | PBST | |
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Dry blend | 28372 - 40 packs |
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Liquid conc. | 28358 - 500 ml |
28348 - 500 ml |
28360 - 500 ml |
28352 - 500 ml |
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Like other immunoassay procedures, Western blotting consists of a series of incubations with different immunochemical reagents separated by wash steps. Washing steps are necessary to remove unbound reagents and reduce background, thereby increasing the signal-to-noise ratio.
Insufficient washing produces high background, while excessive washing may result in decreased sensitivity caused by elution of the antibody and/or antigen from the blot. As with other steps in performing a Western blot, a variety of buffers may be used. Occasionally, washing is performed in a physiological buffer such as Tris-buffered saline (TBS) or phosphate-buffered saline (PBS) without any additives. More commonly, a detergent such as 0.05% Tween-20 Detergent (Product # 28320) is added to the buffer to help remove nonspecifically bound material.
Another common technique is to use a dilute solution of the blocking buffer along with some added detergent to help minimize background. For best results, use high-purity detergents, such as Surfact-Amps™ Detergents for Western blotting.
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For Research Use Only. Not for use in diagnostic procedures.