Combined Protease and Phosphatase Inhibitors

These combined protease and phosphatase inhibitor cocktails and tablets contain chemical compounds that target serine, threonine, and tyrosine phosphatases, as well as serine, cysteine, and aspartic acid proteases, as well as aminopeptidases. Metalloproteases are inhibited by the addition of EDTA, which is available in a separate vial in the liquid format, but included in the tablet format. These broad-spectrum inhibitor formulations prevent protein degradation and preserve phosphorylation simultaneously, helping provide complete protection in a single solution or tablet.

Our Thermo Scientific Haltliquid cocktails are available in a 100 μL single-use format or 1, 5 and 10 mL pack sizes. Thermo Scientific Pierce tablets come in one size for 10 mL volumes, to accommodate different volume/pricing needs. These tablets are formulated to dissolve quickly into a clear solution, and are fully compatible with all Pierce protein assays

Choose the right protease inhibitor cocktail format (liquid or tablet) for your experiment

  Halt Protease and Phosphatase Inhibitor Cocktail Halt Protease and Phosphatase Inhibitor Cocktail, EDTA free Pierce Protease and Phosphatase Inhibitor Tablet Pierce Protease and Phosphatase Inhibitor Tablet, EDTA Free
Flexible addition based on sample volume? Yes Yes No No
Contains EDTA Yes, but in a separate vial No Yes No
Contains DMSO Yes Yes No No
Requires reconstitution No (100X) No (100X) Yes Yes
Downstream compatibility Not compatible with 2D or IMAC All Not compatible with 2D or IMAC All
Product sizes available 24 x 100 μL, 1 mL, 5 x 1 mL, 10 mL 24 x 100 μL, 1 mL, 5 x 1 mL, 10 mL 30 tablets 30 tablets
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A12n04-Fig4

Figure 1. Effective inhibition of protease and phosphatase using the combination tablet. Using the tablet formulation that inhibits protease and phosphatase activity, the degree of enzyme inhibition was determined. A quenched-fluorescent substrate with pancreatic tissue extract was used and fluorescence measured after substrate cleavage. The degree of inhibition is indicated. Panel A: Protease assay reactions were incubated for 2 hours and 37°C. Panel B: Acid phosphatase assay reactions were incubated for 1 hour at 37°C.

Resources

For Research Use Only. Not for use in diagnostic procedures.