Thermo Scientific FastDigest Restriction Enzymes—25% OFF

1 buffer. 176 restriction enzymes. 100% activity

  • 100% activity of all FastDigest™ enzymes in ONE universal reaction buffer
  • Complete digestion in 5 to 15 minutes
  • Green Buffer for direct loading on gels
  • 100% buffer compatibility with downstream applications

Discover all 176 FastDigest enzymes now

Please add "PM" to the end of standard FastDigest product to receive promotional pricing. Offers are valid from September 1, 2015 to December 31, 2015.

Why should you choose FastDigest restriction enzymes instead of conventional restriction enzymes?

  • Choose simplicity
  Conventional restriction enzymes FastDigest restriction enzymes
Buffer system Up to 20 buffers One universal buffer
Buffer change between enzymatic steps Frequently necessary Not necessary
Double/multiple restriction digestion
Limited due to buffer incompatibility Unlimited all 176 enzymes are 100% active in one buffer
Reaction time for plasmid DNA, genomic DNA, PCR product 1 hour—overnight 5—15 minutes
Direct loading of reaction mixture on gels No Yes
Compatibility with downstream applications Partial  100% 
Star activity  Dependent on enzyme properties and reaction conditions No star activity due to short incubation time as per protocol 
Activity definition 1 unit of enzyme hydrolyzes 1 ug of lambda DNA in 60 minutes in an optimal buffer for an enzyme  1 ul of FastDigest enzyme cleaves 1 ug of substrate DNA in 5 or 15 minutes in FastDigest Buffer 
  • Save time
Conventional restriction enzymes
Sequential digestion
FastDigest restriction enzymes
One reaction mixture
Reaction setup for Apal
 ~2 minutes
Reaction set up with FastDigest ApaI and XhoI
~2 minutes
Incubation
60 minutes
Incubation
5 minutes
Reaction setup for Xhol
~2 minutes
--
Incubation
60 minutes
--
TOTAL TIME
>2 hours
TOTAL TIME
 7 minutes
*Please use promotional Cat. No. listed above and refer to the back cover for ordering details. September 1, 2015 to December 31, 2015. Not to be combined with any other promotions or offers.

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