PARP (Cleaved Asp214, Asp215) Antibody (A21969) in WB

Western blot analysis of ADP-ribose Polymerase 1 was performed using nuclear enriched extracts (30 µg lysate) of Jurkat (Lane 1), Jurkat treated with etoposide (100uM Etoposide for 6hrs) (Lane 2) and HEK-293 (Lane 3). The blots were probed with Anti-ADP-ribose Polymerase 1 Mouse Monoclonal Antibody (Product ## A21969,1:250 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product ## A28177, 0.4 µg/ml, 1:2500 dilution). An 85 kDa band corresponding to ADP-ribose Polymerase 1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product ## NP0302BOX), XCell SureLock™ Electrophoresis System (Product ## EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product ## LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product ## IB21001). The membrane was probed with the relevant primary and secondary antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product ## WP20005).

Western blot analysis of ADP-ribose Polymerase 1 was performed using nuclear enriched extracts (30 µg lysate) of Jurkat (Lane 1), Jurkat treated with etoposide (100uM Etoposide for 6hrs) (Lane 2) and HEK-293 (Lane 3). The blots were probed with Anti-ADP-ribose Polymerase 1 Mouse Monoclonal Antibody (Product ## A21969,1:250 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product ## A28177, 0.4 µg/ml, 1:2500 dilution). An 85 kDa band corresponding to ADP-ribose Polymerase 1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product ## NP0302BOX), XCell SureLock™ Electrophoresis System (Product ## EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product ## LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product ## IB21001). The membrane was probed with the relevant primary and secondary antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product ## WP20005).

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