Hot Start PCR Reagents and Kits

Hot Start PCR Reagents and Kits

Hot start PCR reduces non-specific DNA amplification that occurs at room or colder temperatures by preventing key reagents from reacting until optimal temperatures are reached. These products include kits, master mixes, polymerase, and buffers, that require thermal activation and can be used to increase product yield, specificity, and sensitivity.
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Invitrogen Platinum II Hot-Start PCR Master Mix (2X), available in colorless and green formats, offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.
Thermo Scientific DreamTaq Hot Start PCR Master Mix, available in colorless and green formats, is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq Buffer, magnesium, and dNTPs.
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable hot-start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The hot start property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase...
Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.
AmpliTaq Gold™ 360 Master Mix Applied Biosystems™ Green features
AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package with all components premixed and premeasured. Designed for convenience, this master mix scales to various reaction volumes for greater application and format flexibility.
Thermo Scientific DreamTaq Hot Start DNA Polymerase, available in colorless and green formats, is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.
Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA. It contains high-performing, engineered Platinum II Taq Hot-Start DNA Polymerase with dNTPs in an innovative buffer that enables universal primer annealing for...
AmpliTaq Gold DNA Polymerase with Buffer I is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation. The modified enzyme is provided in an inactive state and upon thermal activation, the modifier is permanently released, regenerating active enzyme.
The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with AmpliTaq Gold 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. This polymerase efficiently amplifies targets present at low copy number, even in the presence of high concentrations of...
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation. This modified enzyme is provided in an inactive state and upon thermal activation the modifier is permanently released, regenerating active enzyme.
Applied Biosystems AmpliTaq Gold DNA Polymerase, LD (Low DNA), is a highly purified version of AmpliTaq Gold DNA Polymerase that significantly reduces contaminating bacterial DNA sequences commonly present in recombinant protein preparations.
The PCR Master Mix Starter Pack is composed of three different PCR master mixes for easy evaluation of their suitability for use in your research. These master mixes provide our best DNA polymerases for routine PCR, hot-start PCR, and high-fidelity PCR needs.
Platinum™ Tfi Exo(-) DNA Polymerase
True Allele™ PCR Premix Applied Biosystems™
Applied Biosystems True Allele PCR Premix is a PCR master mix that contains AmpliTaq Gold DNA Polymerase, dNTPs, MgCl2, and buffer. It is intended for use with the ABI PRISM Linkage Mapping Set. True Allele PCR Premix has been optimized for strong, specific PCR amplification of microsatellite...
The Applied Biosystems™ AmpliTaq Gold™ Fast PCR Master Mix is a premix, hot start chemistry for end-point PCR analysis. Its optimized chemical design enables to shorten PCR running time from a traditional 2 hours to proximal 40 minutes.
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Optimized real-time PCR (qPCR) analysis enables sensitive and specific quantification of nucleic acids. Reagent selection plays a critical role in any qPCR or RT-qPCR protocol to help ensure optimal performance and reliable results.
Real-time PCR (qPCR) offers sensitivity, specificity, and wide dynamic range for detecting target nucleic acids, making it a useful and powerful technology for research applications that cover the spectrum of biology from basic research to translational medicine.
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Which end-point PCR reagents do you recommend using with the SuperScript IV CellsDirect cDNA Synthesis Kit?
Application Note: Evaluation of various RNA master mixes in animal health application