Alexa Fluor™ 488 Hydrazide is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins. Alexa Fluor™ 488 is a bright, green fluorescent dye with excitation ideally suited to the 488 nn laser line.
Alexa Fluor™ dyes are reactive molecules that can be used to add a fluorescent label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting Alexa Fluor™ conjugates exhibit brighter fluorescence and greater photostability than...
Thermo Scientific EZ-Link Sulfo-NHS-LC-LC-Biotin enables simple and efficient biotin labeling of antibodies, proteins, and any other primary amine–containing macromolecules. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane...
Alexa Fluor antibody labeling kits provide a convenient means to label antibodies or proteins (>40 kDa) with as many as nine different colored dyes. Conjugated antibodies produce lower background fluorescence with less nonspecific binding and are ideal for multiplexing in a variety of applications.
Alexa Fluor™ 647 is a bright and photostable far-red dye with excitation ideally suited to the 633 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 647 dye is water soluble and pH-insensitive from pH 4 to pH 10.
Thermo Scientific EZ-Link Maleimide-PEG11-Biotin is a long, maleimide-activated, sulfhydryl-reactive biotinylation reagent that includes an 11-unit polyethylene glycol spacer arm for increased water-solubility and reach.
Molecular Probes™ Antibody Conjugate Purification kits efficiently purify antibody conjugates from unconjugated dye or biotin. This particular kit is optimized for the purification of 0.5–1 mg of antibody conjugate.
The amine-reactive quencher, dabcyl succinimidyl ester has a broad and intense visible absorption but no fluorescence making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.
Tandem Mass Tag™ 2-plex (TMTduplex™) Isotopic Label Reagents comprise “light” TMTzero and “heavy” TMT6-127 label reagents while Tandem Mass Tag™ 2-plex (TMTduplex) Isobaric Label Reagents comprise “heavy” TMT2-126 and TMT2-127 Label Reagents for multiplexing.
The Thermo Scientific EZ-Link Maleimide Protein Labeling Kit provides a convenient method to label proteins with varying amounts maleimide for crosslinking or conjugation with a thiol/sulfhydryl-containing moiety.
Thermo Scientific Pierce DST, also called disuccinimidyl tartrate, is a water-soluble crosslinker that contains amine-reactive NHS ester ends around a 4-atom spacer arm, whose central diol can be cleaved by periodate oxidation.
Thermo Scientific Pierce Sulfo-EGS is a water-soluble crosslinker that contains amine-reactive sulfo-NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.
Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5. Features of the EGS crosslinker: • Reactive groups: NHS ester (both ends) • Reactive towards: amino groups (primary...
Thermo Scientific EZ-Link NHS-Desthiobiotin is a short, membrane permeable, amine-reactive labeling reagent whose biotin-like group is elutable from streptavidin, making it ideal for intracellular protein labeling and purification.
Thermo Scientific Pierce Sulfo-KMUS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (16.3 angstroms). Features of Sulfo-KMUS: • Reactive groups: sulfo-NHS ester and maleimide • Reactive toward: amino and...