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CD40 (Soluble) Human ELISA Kit (Invitrogen™)
The human sCD40 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD40.
CD40 is a 48 kD type I glycoprotein and a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary.The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, heparin), amniotic fluid
Sample Volume
50 µL
Reported Application
ELISA
CD40 is a 48 kD type I glycoprotein and a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary.The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, heparin), amniotic fluid
Sample Volume
50 µL
Reported Application
ELISA
IL-18 Human ELISA Kit (Invitrogen™)
The human IL-18 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-18.
Formerly called interferon gamma inducing factor (IGIF), IL-18 is a cytokine that plays an important role in the Th1 response, primarily by its ability to induce IFN gamma production in T cells and natural killer cells.
In terms of structure, IL-18 and IL-1 beta share primary amino acidsequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL- 18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). The activity of the mature IL-18 is closely related to that of IL-1.
The activity of IL-18 is via an IL-18 receptor complex. IL-18 induces gene expression and synthesis of TNF, IL-1, Fas Ligand, and several chemokines. Similar to IL-1, IL-18 participates in both innate and acquired immunity.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume
50 µL
Reported Application
ELISA
Formerly called interferon gamma inducing factor (IGIF), IL-18 is a cytokine that plays an important role in the Th1 response, primarily by its ability to induce IFN gamma production in T cells and natural killer cells.
In terms of structure, IL-18 and IL-1 beta share primary amino acidsequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL- 18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). The activity of the mature IL-18 is closely related to that of IL-1.
The activity of IL-18 is via an IL-18 receptor complex. IL-18 induces gene expression and synthesis of TNF, IL-1, Fas Ligand, and several chemokines. Similar to IL-1, IL-18 participates in both innate and acquired immunity.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume
50 µL
Reported Application
ELISA
IL-18 Human ELISA Kit, CE (Invitrogen™)
The human IL-18 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-18. The human IL-18 ELISA is for in vitro diagnostic use. Not for use in therapeutic procedures.
Formerly called interferon gamma inducing factor (IGIF), IL-18 is a cytokine that plays an important role in the Th1 response, primarily by its ability to induce IFN gamma production in T cells and natural killer cells.
In terms of structure, IL-18 and IL-1 beta share primary amino acid sequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL- 18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). The activity of the mature IL-18 is closely related to that of IL-1.
The activity of IL-18 is via an IL-18 receptor complex. IL-18 induces gene expression and synthesis of TNF, IL-1, Fas Ligand, and several chemokines. Similar to IL-1, IL-18 participates in both innate and acquired immunity.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume
50 µL
Reported Application
ELISA
Formerly called interferon gamma inducing factor (IGIF), IL-18 is a cytokine that plays an important role in the Th1 response, primarily by its ability to induce IFN gamma production in T cells and natural killer cells.
In terms of structure, IL-18 and IL-1 beta share primary amino acid sequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL- 18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). The activity of the mature IL-18 is closely related to that of IL-1.
The activity of IL-18 is via an IL-18 receptor complex. IL-18 induces gene expression and synthesis of TNF, IL-1, Fas Ligand, and several chemokines. Similar to IL-1, IL-18 participates in both innate and acquired immunity.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume
50 µL
Reported Application
ELISA
IL-18 Human Matched Antibody Pair (Invitrogen™)
Each Module Set is sufficient for ten 96-well microtiter plates and are identical to our high quality ready-to-use Conventional ELISAs
Formerly called interferon gamma inducing factor (IGIF), IL-18 is a cytokine that plays an important role in the Th1 response, primarily by its ability to induce IFN gamma production in T cells and natural killer cells.
In terms of structure, IL-18 and IL-1 beta share primary amino acidsequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL- 18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). The activity of the mature IL-18 is closely related to that of IL-1.
The activity of IL-18 is via an IL-18 receptor complex. IL-18 induces gene expression and synthesis of TNF, IL-1, Fas Ligand, and several chemokines. Similar to IL-1, IL-18 participates in both innate and acquired immunity.
Conjugate
Biotin
Reported Application
ELISA
Formerly called interferon gamma inducing factor (IGIF), IL-18 is a cytokine that plays an important role in the Th1 response, primarily by its ability to induce IFN gamma production in T cells and natural killer cells.
In terms of structure, IL-18 and IL-1 beta share primary amino acidsequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL- 18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). The activity of the mature IL-18 is closely related to that of IL-1.
The activity of IL-18 is via an IL-18 receptor complex. IL-18 induces gene expression and synthesis of TNF, IL-1, Fas Ligand, and several chemokines. Similar to IL-1, IL-18 participates in both innate and acquired immunity.
Conjugate
Biotin
Reported Application
ELISA
IL-5 Human Instant ELISA™ Kit (Invitrogen™)
The human IL-5 Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Interleukin-5. The human IL-5 Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum, plasma
Sample Volume
50 µL
Reported Application
ELISA
Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum, plasma
Sample Volume
50 µL
Reported Application
ELISA
IL-5 Human Instant ELISA™ Kit, CE (Invitrogen™)
The human IL-5 Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-5. The human IL-5 Instant ELISA is for in vitro diagnostic use. Not for use in therapeutic procedures.
Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum, plasma
Sample Volume
50 µL
Reported Application
ELISA
Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum, plasma
Sample Volume
50 µL
Reported Application
ELISA
IL-5 Human ELISA Kit (Invitrogen™)
The human IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-5.
Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum
Sample Volume
50 µL
Reported Application
ELISA
Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum
Sample Volume
50 µL
Reported Application
ELISA
IL-18 Human Instant ELISA™ Kit (Invitrogen™)
The human IL-18 Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-18.
Formerly called interferon gamma inducing factor (IGIF), IL-18 is a cytokine that plays an important role in the Th1 response, primarily by its ability to induce IFN gamma production in T cells and natural killer cells.
In terms of structure, IL-18 and IL-1 beta share primary amino acidsequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL- 18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). The activity of the mature IL-18 is closely related to that of IL-1.
The activity of IL-18 is via an IL-18 receptor complex. IL-18 induces gene expression and synthesis of TNF, IL-1, Fas Ligand, and several chemokines. Similar to IL-1, IL-18 participates in both innate and acquired immunity.
Conjugate
HRP
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin), urine
Sample Volume
50 µL
Reported Application
ELISA
Formerly called interferon gamma inducing factor (IGIF), IL-18 is a cytokine that plays an important role in the Th1 response, primarily by its ability to induce IFN gamma production in T cells and natural killer cells.
In terms of structure, IL-18 and IL-1 beta share primary amino acidsequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL- 18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). The activity of the mature IL-18 is closely related to that of IL-1.
The activity of IL-18 is via an IL-18 receptor complex. IL-18 induces gene expression and synthesis of TNF, IL-1, Fas Ligand, and several chemokines. Similar to IL-1, IL-18 participates in both innate and acquired immunity.
Conjugate
HRP
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin), urine
Sample Volume
50 µL
Reported Application
ELISA
CRP Human Instant ELISA™ Kit (Invitrogen™)
The human C-reactive protein Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human C-reactive protein.
C-reactive protein is an acute phase protein exclusively synthesized by hepatocytes in response to cytokines released into circulation by activated leukocytes. Serum and plasma levels of CRP have been shown to rise during response to a wide variety of diseases including bacterial infections, acute phase of rheumatoid arthritis, inflammation of the bile duct. High CRP is also found in Guillain-Barre syndrome patients, in multiple sclerosis, viral infections, tuberculosis, acute infectious hepatitis, necrotic diseases, burned patients and after surgical trauma.
CRP thus has been shown to be a useful indicator of inflammatory processes. CRP levels rise in serum or plasma within 24 to 48 hours after acute tissue damage, peaking at acute stage and decreasing with resolution of inflammation or trauma. In addition to being a marker of inflammatory diseases, CRP turned out to be a predictive value as cardiac marker to assess risk of cardio vascular and peripheral vascular disease.
Conjugate
HRP
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume
100 µL (1:500 prediluted)
Reported Application
ELISA
C-reactive protein is an acute phase protein exclusively synthesized by hepatocytes in response to cytokines released into circulation by activated leukocytes. Serum and plasma levels of CRP have been shown to rise during response to a wide variety of diseases including bacterial infections, acute phase of rheumatoid arthritis, inflammation of the bile duct. High CRP is also found in Guillain-Barre syndrome patients, in multiple sclerosis, viral infections, tuberculosis, acute infectious hepatitis, necrotic diseases, burned patients and after surgical trauma.
CRP thus has been shown to be a useful indicator of inflammatory processes. CRP levels rise in serum or plasma within 24 to 48 hours after acute tissue damage, peaking at acute stage and decreasing with resolution of inflammation or trauma. In addition to being a marker of inflammatory diseases, CRP turned out to be a predictive value as cardiac marker to assess risk of cardio vascular and peripheral vascular disease.
Conjugate
HRP
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume
100 µL (1:500 prediluted)
Reported Application
ELISA
IL-5 Human ELISA Kit, CE (Invitrogen™)
The human IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-5.
Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum
Sample Volume
50 µL
Reported Application
ELISA
Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum
Sample Volume
50 µL
Reported Application
ELISA
IL-18 Human Instant ELISA™ Kit, CE (Invitrogen™)
The human IL-18 Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-18. The human IL-18 Instant ELISA is for in vitro diagnostic use. Not for use in therapeutic procedures.
Formerly called interferon gamma inducing factor (IGIF), IL-18 is a cytokine that plays an important role in the Th1 response, primarily by its ability to induce IFN gamma production in T cells and natural killer cells.
In terms of structure, IL-18 and IL-1 beta share primary amino acidsequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL- 18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). The activity of the mature IL-18 is closely related to that of IL-1.
The activity of IL-18 is via an IL-18 receptor complex. IL-18 induces gene expression and synthesis of TNF, IL-1, Fas Ligand, and several chemokines. Similar to IL-1, IL-18 participates in both innate and acquired immunity.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin), urine
Sample Volume
50 µL
Reported Application
ELISA
Formerly called interferon gamma inducing factor (IGIF), IL-18 is a cytokine that plays an important role in the Th1 response, primarily by its ability to induce IFN gamma production in T cells and natural killer cells.
In terms of structure, IL-18 and IL-1 beta share primary amino acidsequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL- 18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). The activity of the mature IL-18 is closely related to that of IL-1.
The activity of IL-18 is via an IL-18 receptor complex. IL-18 induces gene expression and synthesis of TNF, IL-1, Fas Ligand, and several chemokines. Similar to IL-1, IL-18 participates in both innate and acquired immunity.
Conjugate
Biotin
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin), urine
Sample Volume
50 µL
Reported Application
ELISA
CRP Human Instant ELISA™ Kit, CE (Invitrogen™)
The human C-reactive protein Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human C-reactive. The human C-reactive protein Instant ELISA is for in vitro diagnostic use. Not for or therapeutic procedures.
C-reactive protein is an acute phase protein exclusively synthesized by hepatocytes in response to cytokines released into circulation by activated leukocytes. Serum and plasma levels of CRP have been shown to rise during response to a wide variety of diseases including bacterial infections, acute phase of rheumatoid arthritis, inflammation of the bile duct. High CRP is also found in Guillain-Barre syndrome patients, in multiple sclerosis, viral infections, tuberculosis, acute infectious hepatitis, necrotic diseases, burned patients and after surgical trauma.
CRP thus has been shown to be a useful indicator of inflammatory processes. CRP levels rise in serum or plasma within 24 to 48 hours after acute tissue damage, peaking at acute stage and decreasing with resolution of inflammation or trauma. In addition to being a marker of inflammatory diseases, CRP turned out to be a predictive value as cardiac marker to assess risk of cardio vascular and peripheral vascular disease.
Conjugate
HRP
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume
100 µL (1:500 prediluted)
Reported Application
ELISA
C-reactive protein is an acute phase protein exclusively synthesized by hepatocytes in response to cytokines released into circulation by activated leukocytes. Serum and plasma levels of CRP have been shown to rise during response to a wide variety of diseases including bacterial infections, acute phase of rheumatoid arthritis, inflammation of the bile duct. High CRP is also found in Guillain-Barre syndrome patients, in multiple sclerosis, viral infections, tuberculosis, acute infectious hepatitis, necrotic diseases, burned patients and after surgical trauma.
CRP thus has been shown to be a useful indicator of inflammatory processes. CRP levels rise in serum or plasma within 24 to 48 hours after acute tissue damage, peaking at acute stage and decreasing with resolution of inflammation or trauma. In addition to being a marker of inflammatory diseases, CRP turned out to be a predictive value as cardiac marker to assess risk of cardio vascular and peripheral vascular disease.
Conjugate
HRP
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume
100 µL (1:500 prediluted)
Reported Application
ELISA
Galectin 3 Human ELISA Kit (Invitrogen™)
The human Galectin-3 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Galectin-3.
Galectin-3 is a 26kDa -galactoside-binding protein belonging to the Galectin family, which consists of more than ten members. Galectin-3 is composed of a carboxyl-terminal carbohydrate recognition domain (CRD) and amino-terminal tandem repeats. Galectin-3 normally distributes in epithelia of many organs and various inflammatory cells, including macrophages as well as dendritic cells and Kupfer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation and cell differentiation and through transactivation by viral proteins. Galectin-3 has been shown to function through both intracellular and extracellular actions. It is a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing and has been found to control cell cycle and prevent T-cell apoptosis through interaction with the Bcl-2 family members. On the other hand, this protein, which is secreted from monocytes/macrophages and epithelial cells has been demonstrated to function as an extracellular molecule in activating various types of cells such as monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions and acts as a novel chemoattractant for monocytes and macrophages.
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA)
Sample Volume
50 µL
Reported Application
ELISA
Galectin-3 is a 26kDa -galactoside-binding protein belonging to the Galectin family, which consists of more than ten members. Galectin-3 is composed of a carboxyl-terminal carbohydrate recognition domain (CRD) and amino-terminal tandem repeats. Galectin-3 normally distributes in epithelia of many organs and various inflammatory cells, including macrophages as well as dendritic cells and Kupfer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation and cell differentiation and through transactivation by viral proteins. Galectin-3 has been shown to function through both intracellular and extracellular actions. It is a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing and has been found to control cell cycle and prevent T-cell apoptosis through interaction with the Bcl-2 family members. On the other hand, this protein, which is secreted from monocytes/macrophages and epithelial cells has been demonstrated to function as an extracellular molecule in activating various types of cells such as monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions and acts as a novel chemoattractant for monocytes and macrophages.
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA)
Sample Volume
50 µL
Reported Application
ELISA
IL-31 Mouse ELISA Kit (Invitrogen™)
The mouse IL-31 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-31.
IL-31 is a 25kD and 163 AA alpha-helical protein, preferentially produced by CD 4+ type 2 helper T cells. IL-31 is closely related to the IL-6 type cytokines such as oncostatin M, Leukemia inhibitory factor and cardiotrophin 1. IL-31 signals via binding to a heterodimeric complex that is composed of IL-31 receptor A (IL-31RA) that is highly homologous with the gp 30-like receptor and oncostatin M receptor subunits.
These receptor subunits are expressed in activated monocytes, in epithelial cells, and keratinocytes respectively.
On target cells IL-31 binding can induce activation of the JAK/STAT, AKT and MAPK signaling pathways. Due to the ubiquitous expression of its receptor complex, IL-31 has numerous physiological roles including regulation of hematopoiesis and immune response.
When overexpressed in transgenic mice, IL-31 induces severe pruritus resembling eczema in mouses. Serum IL-31 was previously found overexpressed in adults with atopic dermatitis (AD). Serum measurement of IL-31 in paediatric atopic dermatitis revealed that elevated levels correlate with severity scoring. Activated effector T cells are recruited to the skin by chemokines and cell adhesion molecules. These components in the local microenvironment prolong T cell survival and contribute to the chronicity of these diseases.
IL-31 is associated with the promotion of allergic and chronic inflammatory conditions such as dermatitis, pruritus, airway hypersensititvity and inflammatory bowel disease.
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume
50 µL
Reported Application
ELISA
IL-31 is a 25kD and 163 AA alpha-helical protein, preferentially produced by CD 4+ type 2 helper T cells. IL-31 is closely related to the IL-6 type cytokines such as oncostatin M, Leukemia inhibitory factor and cardiotrophin 1. IL-31 signals via binding to a heterodimeric complex that is composed of IL-31 receptor A (IL-31RA) that is highly homologous with the gp 30-like receptor and oncostatin M receptor subunits.
These receptor subunits are expressed in activated monocytes, in epithelial cells, and keratinocytes respectively.
On target cells IL-31 binding can induce activation of the JAK/STAT, AKT and MAPK signaling pathways. Due to the ubiquitous expression of its receptor complex, IL-31 has numerous physiological roles including regulation of hematopoiesis and immune response.
When overexpressed in transgenic mice, IL-31 induces severe pruritus resembling eczema in mouses. Serum IL-31 was previously found overexpressed in adults with atopic dermatitis (AD). Serum measurement of IL-31 in paediatric atopic dermatitis revealed that elevated levels correlate with severity scoring. Activated effector T cells are recruited to the skin by chemokines and cell adhesion molecules. These components in the local microenvironment prolong T cell survival and contribute to the chronicity of these diseases.
IL-31 is associated with the promotion of allergic and chronic inflammatory conditions such as dermatitis, pruritus, airway hypersensititvity and inflammatory bowel disease.
Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume
50 µL
Reported Application
ELISA
IgG2a Mouse Uncoated ELISA Kit with Plates (Invitrogen™)
This Mouse IgG2a Uncoated ELISA Kit with Plates contains the necessary reagents, standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). This ELISA set is specifically engineered for accurate and precise measurement of mouse IgG2a.
Suitable Sample Types
Cell culture supernatant, serum and plasma
Sample Volume
50 µL (prediluted 1:10,000)
Reported Application
ELISA
Suitable Sample Types
Cell culture supernatant, serum and plasma
Sample Volume
50 µL (prediluted 1:10,000)
Reported Application
ELISA
