Shop All B-27, G-5, and N-2 Supplements
B-27™ Supplement, minus insulin (Gibco™)
Le supplément B-27® moins insuline est le supplément complet B-27® sans insuline. Il soutient la croissance des neurones du SNC et d’autres cellules dans les applications où la présence d’insuline est source de perturbations.
Pour les études neurales dans lesquelles l’insuline, le glucose et le pyruvate de sodium doivent être absents, nous recommandons Neurobasal®-A sans D-glucose et sans pyruvate de sodium (No de catalogue 0050128DJ) comme milieu de base à utiliser en conjonction avec ce produit.
Pour d’autres applications, nous fournissons des formulations alternatives au supplément B-27®.
Pour les études neurales dans lesquelles l’insuline, le glucose et le pyruvate de sodium doivent être absents, nous recommandons Neurobasal®-A sans D-glucose et sans pyruvate de sodium (No de catalogue 0050128DJ) comme milieu de base à utiliser en conjonction avec ce produit.
Pour d’autres applications, nous fournissons des formulations alternatives au supplément B-27®.
B-27™ Supplement (50X), custom (Gibco™)
Le supplément B-27® (50X) personnalisé est un additif exempt de sérum permettant la croissance et l’expansion des cellules ganglionnaires rétinales. Il est fourni sous forme de concentré liquide 50X et est utilisé avec un milieu basal, tel que le Neurobasal®, ainsi qu’avec d’autres additifs, tels que le GlutaMAX-1.
B-27™ Supplement (50X), minus vitamin A (Gibco™)
B-27™ Supplement Minus Vitamin A is a customized B-27 Supplement without vitamin A. Vitamin A (retinol) can be converted to retinoic acid, which can induce neuronal differentiation. The absence of vitamin A makes this formulation ideal for use in the culture of stem cells.
B-27 Supplement Minus Vitamin A is provided as a 50X liquid and is intended to be used with Neurobasal™ Medium or Neurobasal™-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.
For other applications, we provide alternative formulations of B-27 Supplement.
Quality control testing
B-27 Supplement is tested in a growth assay using primary rat (Sprague Dawley) embryonic hippocampal neurons, 18-day gestation. Additional standard evaluations are for the absence of bacterial and fungal contaminants. B-27 Supplement is also tested for endotoxin at a 1X concentration.
B-27 Supplement Minus Vitamin A is provided as a 50X liquid and is intended to be used with Neurobasal™ Medium or Neurobasal™-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.
For other applications, we provide alternative formulations of B-27 Supplement.
Quality control testing
B-27 Supplement is tested in a growth assay using primary rat (Sprague Dawley) embryonic hippocampal neurons, 18-day gestation. Additional standard evaluations are for the absence of bacterial and fungal contaminants. B-27 Supplement is also tested for endotoxin at a 1X concentration.
B-27™ Supplement (50X), serum free (Gibco™)
Le supplément B-27® est un supplément optimisé sans sérum utilisé pour prendre en charge la croissance haute ou basse densité et la viabilité à court terme ou à long terme des neurones hippocampiques et autres CNS. Le supplément B-27® est fourni sous forme de liquide 50X et doit être utilisé avec les milieux de culture Neurobasal® ou Neurobasal®-A pour la culture de cellules neuronales sans nécessiter de couche nourricière d’astrocytes.
Le supplément B-27® convient à la plupart des applications relatives aux cellules neuronales. Pour des applications spécialisées et en fonction de vos besoins expérimentaux, nous fournissons des formulations alternatives au supplément B-27®.
Test de contrôle qualité
Le supplément B-27® est testé dans une analyse de croissance utilisant des neurones hippocampiques embryonnaires primaires de rats (Sprague Dawley), à 18 jours de gestation. Des évaluations standard complémentaires permettent de vérifier l’absence de contaminants bactériens et fongiques. Le supplément B-27® est également testé pour rechercher les endotoxines à une concentration de 1X.
Usage exclusivement réservé à la recherche. Le supplément n’est pas prévu à des fins thérapeutiques humaines ou animales ni pour une utilisation diagnostique, sauf indication contraire.
Le supplément B-27® convient à la plupart des applications relatives aux cellules neuronales. Pour des applications spécialisées et en fonction de vos besoins expérimentaux, nous fournissons des formulations alternatives au supplément B-27®.
Test de contrôle qualité
Le supplément B-27® est testé dans une analyse de croissance utilisant des neurones hippocampiques embryonnaires primaires de rats (Sprague Dawley), à 18 jours de gestation. Des évaluations standard complémentaires permettent de vérifier l’absence de contaminants bactériens et fongiques. Le supplément B-27® est également testé pour rechercher les endotoxines à une concentration de 1X.
Usage exclusivement réservé à la recherche. Le supplément n’est pas prévu à des fins thérapeutiques humaines ou animales ni pour une utilisation diagnostique, sauf indication contraire.
CTS™ B-27™ Supplement, XenoFree, minus insulin (Gibco™)
CTS B-27 Supplement XenoFree without Insulin is based on the classic B-27 Supplement formulation and contains no animal-derived components at the primary component level. The absence of insulin makes this formulation ideal for the growth of hippocampal and other CNS neurons in applications where the presence of insulin causes interference or in the derivation of cardiomyocytes and mesodermal cells from stem cells. CTS B-27 Supplement XenoFree without Insulin reduces the burden in qualifying reagents when transitioning from the research bench to the clinic.
CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.
Formulated xeno-free
The preparation contains no animal-derived components at the primary component level.
Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.
CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.
Formulated xeno-free
The preparation contains no animal-derived components at the primary component level.
Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.
N-2 Supplement (100X) (Gibco™)
N-2 Supplement is a chemically-defined, serum-free supplement based on Bottenstein’s N-1 formulation. N-2 Supplement is intended for use with Neurobasal™ media.
N-2 Supplement is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system and the central nervous system. N-2 Supplement can be used as a substitute for Bottenstein’s N-1 formulation. N-2 Supplement is provided as a 100X concentrate.
N-2 Supplement may be used with Neurobasal media supplemented with growth factors such as bFGF and EGF or used with DMEM.
Learn more about the N-2 Supplement formulation >
Learn more about Invitrogen™ neural cell culture products >
N-2 Supplement is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system and the central nervous system. N-2 Supplement can be used as a substitute for Bottenstein’s N-1 formulation. N-2 Supplement is provided as a 100X concentrate.
N-2 Supplement may be used with Neurobasal media supplemented with growth factors such as bFGF and EGF or used with DMEM.
Learn more about the N-2 Supplement formulation >
Learn more about Invitrogen™ neural cell culture products >
CTS™ (Cell Therapy Systems) N-2 Supplement (Gibco™)
Cell Therapy Systems N-2 CTS™ (100X) Supplement is a serum-free supplement for the growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. Cell Therapy Systems N-2 CTS™ (100X) supplement is a chemically defined, 100X concentrate of Bottenstein's N-1 formulation.
Serum-free supplement to be used with Neurobasal® CTS™ Media
• Substitute for Bottenstein’s N-1 formulation.
• Be ready for clinical research with this cGmP manufactured formula and support documentation
Use with Neurobasal® CTS™ Media for Nueronal Cell Culturing
Cell Therapy Systems N-2 CTS™ (100X) Supplement is recommended for the growth and expression of neuroblastomas and for the survival and expression of post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). Cell Therapy Systems N-2 CTS™ (100X) Supplement is intended for use with Cell Therapy Systems Neurobasal® CTS™ media.
Selective for Neuronal Cell Lines
It can be used as a substitute for the N1 Bottenstein formulation. Cell Therapy Systems N-2 CTS™ (100X) supplement appears to be selective for neuronal cell lines and does not support the growth of nonneuronal cell lines.
Media Formulation Details
N-2 supplement is a chemically defined, 100X concentrate of Bottenstein's N-2 formulation. For component concentrations of the media supplement see our technical resource page for the N-2 supplement.
Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)
For Research Use or Non-Commercial Manufacturing of Cell-Based Products for Clinical Research.
CAUTION: Not intended for direct administration to humans or animals
Serum-free supplement to be used with Neurobasal® CTS™ Media
• Substitute for Bottenstein’s N-1 formulation.
• Be ready for clinical research with this cGmP manufactured formula and support documentation
Use with Neurobasal® CTS™ Media for Nueronal Cell Culturing
Cell Therapy Systems N-2 CTS™ (100X) Supplement is recommended for the growth and expression of neuroblastomas and for the survival and expression of post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). Cell Therapy Systems N-2 CTS™ (100X) Supplement is intended for use with Cell Therapy Systems Neurobasal® CTS™ media.
Selective for Neuronal Cell Lines
It can be used as a substitute for the N1 Bottenstein formulation. Cell Therapy Systems N-2 CTS™ (100X) supplement appears to be selective for neuronal cell lines and does not support the growth of nonneuronal cell lines.
Media Formulation Details
N-2 supplement is a chemically defined, 100X concentrate of Bottenstein's N-2 formulation. For component concentrations of the media supplement see our technical resource page for the N-2 supplement.
Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)
For Research Use or Non-Commercial Manufacturing of Cell-Based Products for Clinical Research.
CAUTION: Not intended for direct administration to humans or animals
G-5 Supplement (100X) (Gibco™)
G-5 Supplement is a chemically defined, serum-free supplement based on Bottenstein’s G-5 formulation. G-5 Supplement is intended for use with Neurobasal® media.
G-5 Supplement is recommended for growth and expression of glial cells (normal and tumor) of astrocytic phenotype (astrocytes). G-5 Supplement can be used as a substitute for Bottenstein’s G-2 formulation. G-5 Supplement is provided as a 100X concentrate.
G-5 Supplement may be used with Neurobasal® media, DMEM, DMEM⁄F-12, or other basal media..
Learn more about G-5 Supplement formulation.
Learn more about neural cell culture products from Invitrogen at www.invitrogen.com/neuralculture.
For Research Use Only. Not intended for any animal or human therapeutic of diagnostic use.
G-5 Supplement is recommended for growth and expression of glial cells (normal and tumor) of astrocytic phenotype (astrocytes). G-5 Supplement can be used as a substitute for Bottenstein’s G-2 formulation. G-5 Supplement is provided as a 100X concentrate.
G-5 Supplement may be used with Neurobasal® media, DMEM, DMEM⁄F-12, or other basal media..
Learn more about G-5 Supplement formulation.
Learn more about neural cell culture products from Invitrogen at www.invitrogen.com/neuralculture.
For Research Use Only. Not intended for any animal or human therapeutic of diagnostic use.
B-27™ Supplement (50X), minus antioxidants (Gibco™)
B-27® Supplement Minus AO is a customized B-27® Supplement without 5 antioxidants (vitamin E, vitamin E acetate, superoxide dismutase, catalase, and glutathione). Antioxidants serve as free radical scavengers, and their absence makes this formulation ideal for use in studies of oxidative damage to neurons.
B-27® Supplement Minus AO is provided as a 50X liquid and is intended to be used with Neurobasal® Medium or Neurobasal®-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.
B-27® Supplement is suitable for use in most neuronal cell applications. For specialized applications and depending on your experimental needs, we provide alternative formulations of B-27® Supplement.
Quality Control Testing
B-27® Supplement is tested in a growth assay utilizing primary rat (Sprague Dawley) embryonic hippocampal neurons, 18 day gestation. Additional standard evaluations are for the absence of bacterial and fungal contaminants. B-27® is also tested for endotoxin at a 1X concentration.
B-27® Supplement Minus AO is provided as a 50X liquid and is intended to be used with Neurobasal® Medium or Neurobasal®-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.
B-27® Supplement is suitable for use in most neuronal cell applications. For specialized applications and depending on your experimental needs, we provide alternative formulations of B-27® Supplement.
Quality Control Testing
B-27® Supplement is tested in a growth assay utilizing primary rat (Sprague Dawley) embryonic hippocampal neurons, 18 day gestation. Additional standard evaluations are for the absence of bacterial and fungal contaminants. B-27® is also tested for endotoxin at a 1X concentration.
CTS™ B-27™ Supplement, XenoFree (Gibco™)
From the Cell Therapy Systems (CTS™) line of products, B-27® Supplement, XenoFree CTS™, is B-27® Serum-Free Supplement formulated with only recombinant or humanized components. Designed with the translational stem cell researcher in mind, this B-27® supplement can be used to support induction of human neural stem cells (hNSCs) from pluripotent stem cells (PSCs), expansion of hNSCs, differentiation of hNSCs, and maintenance of mature differentiated neurons in culture.
• Validated—designed for use with the CTS™ line of neural cell culture products
• High performance—performs equivalently to B-27® Serum-Free Supplement, the gold standard for neural cell culture
• Expanded use—for research use or manufacturing of cell-, gene-, or tissue-based products
End-to-End Solution
When used in conjunction with other CTS™ neural cell culture products, B-27® Supplement, XenoFree CTS™, has been validated for use in all four major steps of neural stem cell culture: induction, expansion, differentiation, and maintenance of mature, differentiated neurons (Figure 1).
B-27® Brand Quality and Reliability
Using advanced manufacturing methods and the highest quality raw materials, B-27® Supplement, XenoFree CTS™, performs equivalently to B-27® Serum-Free Supplement (Figure 2).
Gibco® CTS Promise
Gibco® CTS™ brand products comprise a broad array of products for use in cell therapy research applications. CTS™ products are manufactured following GMP 21 CFR Part 864, subpart C, and ISO 9001/13485 and deliver reduced burden in qualifying reagents when transitioning from the research bench to the clinic. Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available, where applicable.
• Validated—designed for use with the CTS™ line of neural cell culture products
• High performance—performs equivalently to B-27® Serum-Free Supplement, the gold standard for neural cell culture
• Expanded use—for research use or manufacturing of cell-, gene-, or tissue-based products
End-to-End Solution
When used in conjunction with other CTS™ neural cell culture products, B-27® Supplement, XenoFree CTS™, has been validated for use in all four major steps of neural stem cell culture: induction, expansion, differentiation, and maintenance of mature, differentiated neurons (Figure 1).
B-27® Brand Quality and Reliability
Using advanced manufacturing methods and the highest quality raw materials, B-27® Supplement, XenoFree CTS™, performs equivalently to B-27® Serum-Free Supplement (Figure 2).
Gibco® CTS Promise
Gibco® CTS™ brand products comprise a broad array of products for use in cell therapy research applications. CTS™ products are manufactured following GMP 21 CFR Part 864, subpart C, and ISO 9001/13485 and deliver reduced burden in qualifying reagents when transitioning from the research bench to the clinic. Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available, where applicable.
CTS™ B-27™ Supplement, XenoFree, minus vitamin A (Gibco™)
CTS B-27 Supplement XenoFree without Vitamin A is based on the classic B-27 Supplement formulation and contains no animal-derived components at the primary component level. The absence of vitamin A (retinyl acetate) makes this formulation ideal for the cultivation of neural progenitor and stem cells, either as neurospheres in suspension or in adherent monolayer culture, without inducing differentiation. CTS B-27 Supplement XenoFree without Vitamin A reduces the burden in qualifying reagents when transitioning from the research bench to the clinic.
CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.
Formulated xeno-free
The preparation contains no animal-derived components at the primary component level.
Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.
CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.
Formulated xeno-free
The preparation contains no animal-derived components at the primary component level.
Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.
