Shop All Serum-Free & Specialty Media
Neurobasal™-A Medium, no D-glucose, no sodium pyruvate (Gibco™)
This is complete Neurobasal®-A medium without D-glucose and sodium pyruvate, and it is used to support growth of post-natal CNS neurons in applications where the presence of D-glucose and sodium pyruvate can cause interference.
For neural studies that require the absence of insulin, glucose, and sodium pyruvate, such as glucose response or insulin response studies, we recommend B-27® Supplement Minus Insulin as a supplement to this medium.
For other applications, we provide alternative formulations of B-27® Supplement.
For neural studies that require the absence of insulin, glucose, and sodium pyruvate, such as glucose response or insulin response studies, we recommend B-27® Supplement Minus Insulin as a supplement to this medium.
For other applications, we provide alternative formulations of B-27® Supplement.
iPSC Lab Starter Kit with Reprogramming (Gibco™)
The Gibco™ iPSC Lab Starter Kit with Reprogramming provides researchers initiating pluripotent stem cell research with the required reagents at a significant discount over purchasing them separately. This package solution is appropriate for labs that plan to perform reprogramming of donor-derived fibroblasts . For labs that are starting from cryopreserved pluripotent stem cells, please see the Gibco™ iPSC Lab Starter Kit, which does not include reprogramming reagents.
The iPSC Lab Starter Kit with Reprogramming includes the following products:
Reprogramming:
• CytoTune-iPS 2.0 Sendai Reprogramming Kit, Cat. No. A16517, 1 kit
• High glucose DMEM with GlutaMAX™-I, Cat. No. 10569-044, 40 x 500 mL
• Gibco™ Mouse Embryonic Fibroblasts (Irradiated), Cat. No. A24903, 11 vials
• MEM Non-Essential Amino Acids Solution (100X), Cat. No. 11140-050, 100 mL
• Fetal Bovine Serum, ES Cell-Qualified, Cat. No. 16141-061, 2 x 100 mL
• Attachment Factor, Cat. No. S-006-100, 2 x 100 mL
• 2-Mercaptoethanol (55 mM), Cat. No. 21985-023, 50 mL
• DMEM/F-12 with GlutaMAX™, Cat. No. 10565-018, 5 x 500 mL
• KnockOut™ Serum Replacement, Cat. No. 10828-010, 3 x 100 mL
• FGF-basic (AA 1-155) Recombinant Human Protein, Cat. No. PHG0264, 4 vials
• Collagenase Type IV, Cat. No. 17104-019, 1 vial
• Tra-1-60 AlexaFluor™ 488 Conjugate for Live Staining, Cat. No. A25618, 1 kit
PSC feeder-free transition and expansion:
• Essential 8™ Adaptation Kit, Cat. No. A25953, 1 kit
• Essential 8™ Medium, Cat. No. A1517001, 4 x 500 mL
• Geltrex™ hESC-qualified Basement Membrane Extract, Cat. No. A1413302, 2 x 5 mL
• Vitronectin (VTN-N), Cat. No. A14700, 3 x 1 mL
• Versene Solution, Cat. No. 15040066, 100 mL
• Dulbecco’s Phosphate Buffered Saline without Ca2+/Mg2+, Cat. No 14190250, 20 x 500 mL
The iPSC Lab Starter Kit with Reprogramming includes the following products:
Reprogramming:
• CytoTune-iPS 2.0 Sendai Reprogramming Kit, Cat. No. A16517, 1 kit
• High glucose DMEM with GlutaMAX™-I, Cat. No. 10569-044, 40 x 500 mL
• Gibco™ Mouse Embryonic Fibroblasts (Irradiated), Cat. No. A24903, 11 vials
• MEM Non-Essential Amino Acids Solution (100X), Cat. No. 11140-050, 100 mL
• Fetal Bovine Serum, ES Cell-Qualified, Cat. No. 16141-061, 2 x 100 mL
• Attachment Factor, Cat. No. S-006-100, 2 x 100 mL
• 2-Mercaptoethanol (55 mM), Cat. No. 21985-023, 50 mL
• DMEM/F-12 with GlutaMAX™, Cat. No. 10565-018, 5 x 500 mL
• KnockOut™ Serum Replacement, Cat. No. 10828-010, 3 x 100 mL
• FGF-basic (AA 1-155) Recombinant Human Protein, Cat. No. PHG0264, 4 vials
• Collagenase Type IV, Cat. No. 17104-019, 1 vial
• Tra-1-60 AlexaFluor™ 488 Conjugate for Live Staining, Cat. No. A25618, 1 kit
PSC feeder-free transition and expansion:
• Essential 8™ Adaptation Kit, Cat. No. A25953, 1 kit
• Essential 8™ Medium, Cat. No. A1517001, 4 x 500 mL
• Geltrex™ hESC-qualified Basement Membrane Extract, Cat. No. A1413302, 2 x 5 mL
• Vitronectin (VTN-N), Cat. No. A14700, 3 x 1 mL
• Versene Solution, Cat. No. 15040066, 100 mL
• Dulbecco’s Phosphate Buffered Saline without Ca2+/Mg2+, Cat. No 14190250, 20 x 500 mL
PB-MAX™ Karyotyping Medium (Gibco™)
Gibco® PB-MAX™ Karyotyping Medium is a fully-supplemented medium developed for the short-term culture of peripheral blood lymphocytes for cytogenetic studies and in vitro diagnostic procedures.
Gibco® PB-MAX™ Karyotyping Medium features:
• Ready-to-use format
• Quality and performance testing
• Classic formulation
Ready-to-use format
Gibco® PB-MAX™ Karyotyping Medium is a frozen, 1X medium, ready to use upon thawing. No supplementation is required. Thawed medium can be stored at 2–8°C for up to 14 days.
Quality and performance testing
Every lot of Gibco® PB-MAX™ Karyotyping Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Peripheral blood lymphocytes from a normal adult donor are cultured for 72 hours in Gibco® PB-MAX™ Karyotyping Medium before measuring the mitotic index and chromosome banding resolution. In addition, each lot is tested for sterility, pH, osmolality, and mycoplasma.
Classic formulation
Gibco® PB-MAX™ Karyotyping Medium is an optimized RPMI 1640 medium, supplemented with Fetal Bovine Serum (FBS), L-glutamine, and phytohemagglutinin (PHA). Gibco® PB-MAX™ Karyotyping Medium is based on formulations referenced in the Association for Cytogenetic Technologists Laboratory Manual for the culture of peripheral blood lymphocytes for cytogenetic analysis. The complete formulation is available.
Product Use
For in vitro diagnostic use.
cGMP Manufacturing and Quality System
Gibco® PB-MAX™ Karyotyping Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Gibco® PB-MAX™ Karyotyping Medium features:
• Ready-to-use format
• Quality and performance testing
• Classic formulation
Ready-to-use format
Gibco® PB-MAX™ Karyotyping Medium is a frozen, 1X medium, ready to use upon thawing. No supplementation is required. Thawed medium can be stored at 2–8°C for up to 14 days.
Quality and performance testing
Every lot of Gibco® PB-MAX™ Karyotyping Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Peripheral blood lymphocytes from a normal adult donor are cultured for 72 hours in Gibco® PB-MAX™ Karyotyping Medium before measuring the mitotic index and chromosome banding resolution. In addition, each lot is tested for sterility, pH, osmolality, and mycoplasma.
Classic formulation
Gibco® PB-MAX™ Karyotyping Medium is an optimized RPMI 1640 medium, supplemented with Fetal Bovine Serum (FBS), L-glutamine, and phytohemagglutinin (PHA). Gibco® PB-MAX™ Karyotyping Medium is based on formulations referenced in the Association for Cytogenetic Technologists Laboratory Manual for the culture of peripheral blood lymphocytes for cytogenetic analysis. The complete formulation is available.
Product Use
For in vitro diagnostic use.
cGMP Manufacturing and Quality System
Gibco® PB-MAX™ Karyotyping Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
PFHM-II Protein-Free Hybridoma Medium (Gibco™)
Gibco® Protein Free Hybridoma Medium II (PFHM II) is an animal origin-free, serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production.
Gibco® PFHM II Features:
• Ability to support suspension and stationary hybridomas and myelomas
• Complete, ready-to-use formulation
• Animal origin-free, serum-free, protein-free formulation
Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250X Cholesterol Lipid Concentrate.
Complete, ready-to-use formulation
Gibco® PFHM II is a complete medium that is ready-to-use. No supplementation is required.
Animal origin-free, serum-free, protein-free formulation
Gibco® PFHM II is an animal origin-free, serum-free, protein-free formulation, allowing for easier purification of your protein of interest.
Product Use
For Research Use Only. Not for use in diagnostic procedures.
cGMP Manufacturing and Quality System
Gibco® PFHM II is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® PFHM II product made in our Scotland facility (12040-051). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Gibco® PFHM II Features:
• Ability to support suspension and stationary hybridomas and myelomas
• Complete, ready-to-use formulation
• Animal origin-free, serum-free, protein-free formulation
Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250X Cholesterol Lipid Concentrate.
Complete, ready-to-use formulation
Gibco® PFHM II is a complete medium that is ready-to-use. No supplementation is required.
Animal origin-free, serum-free, protein-free formulation
Gibco® PFHM II is an animal origin-free, serum-free, protein-free formulation, allowing for easier purification of your protein of interest.
Product Use
For Research Use Only. Not for use in diagnostic procedures.
cGMP Manufacturing and Quality System
Gibco® PFHM II is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® PFHM II product made in our Scotland facility (12040-051). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
iPSC Lab Starter Kit (Gibco™)
The Gibco™ iPSC Lab Starter Kit provides researchers initiating pluripotent stem cell research with the required reagents at a significant discount over purchasing them separately. This package is appropriate for labs that are starting from cryopreserved pluripotent stem cells previously adapted to culture in Essential 8™ medium. When starting from cells that are adapted to other feeder-based or feeder-free culture systems, please add the Essential 8™ Adaptation Kit (sold separately).For labs that plan to perform reprogramming of donor-derived fibroblasts , please see the Gibco™ iPSC Lab Starter Kit with Reprogramming.
The iPSC Lab Starter Kit includes the following products for PSC growth and expansion:
• Essential 8™ Medium, Cat. No. A1517001, 6 x 500 mL
• Geltrex™ hESC-qualified Basement Membrane Extract, Cat. No. A1413302, 2 x 5 mL
• Vitronectin (VTN-N), Cat. No. A14700, 4 x 1 mL
• Versene Solution, Cat. No. 15040066, 2 x 100 mL
• Dulbecco’s Phosphate Buffered Saline without Ca2+/Mg2+, Cat. No 14190250, 30 x 500 mL
The iPSC Lab Starter Kit includes the following products for PSC growth and expansion:
• Essential 8™ Medium, Cat. No. A1517001, 6 x 500 mL
• Geltrex™ hESC-qualified Basement Membrane Extract, Cat. No. A1413302, 2 x 5 mL
• Vitronectin (VTN-N), Cat. No. A14700, 4 x 1 mL
• Versene Solution, Cat. No. 15040066, 2 x 100 mL
• Dulbecco’s Phosphate Buffered Saline without Ca2+/Mg2+, Cat. No 14190250, 30 x 500 mL
Hybridoma-SFM (Gibco™)
Hybridoma-SFM est un milieu exempt de sérum et contenant très peu de protéines spécifiquement élaboré pour prendre en charge la croissance des hybridomes pour la production d’anticorps. Caractéristique du Hybridoma-SFM :
• Capacité à soutenir les hybridomes et les myélomes stationnaires et en suspension
• Formulation complète, prête à l’emploi
• Formulation exempte de sérum et contenant très peu de protéines
Capacité à soutenir les hybridomes et les myélomes stationnaires et en suspension
Adapté à la culture de lignées de myélomes recombinants ainsi que d’hybridomes classiques, Hybridoma-SFM offre des densités cellulaires et une production d’IgG élevées. Les cultures dépendantes du cholestérol telles que le NS0 nécessitent l’ajout de concentré lipide cholestérol 250X.
Formulation complète, prête à l’emploi
Hybridoma-SFM est un milieu complet prêt à l’emploi. Aucune supplémentation n’est nécessaire.
Formulation exempte de sérum et contenant très peu de protéines
Hybridoma-SFM est un milieu exempt de sérum et contenant très peu de protéines facilitant la purification de votre protéine d’intérêt. Hybridoma-SFM contient un taux de protéines totales < 20 µg/ml.
Utilisation du produit
Les clients qui utilisent le milieu Gibco® Hybridoma-SFM dans un procédé de fabrication, et ayant soumis une demande à la FDA, peuvent nous demander une lettre d’autorisation pour référencer notre Drug Master File ou DMF (fichier maître des médicaments) de type II.
Fabrication et système de qualité conformes aux BPFa
Le milieu Hybridoma-SFM est fabriqué dans un site conforme aux BPFa situé à Grand Island, New York (États-Unis). Le site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485.
• Capacité à soutenir les hybridomes et les myélomes stationnaires et en suspension
• Formulation complète, prête à l’emploi
• Formulation exempte de sérum et contenant très peu de protéines
Capacité à soutenir les hybridomes et les myélomes stationnaires et en suspension
Adapté à la culture de lignées de myélomes recombinants ainsi que d’hybridomes classiques, Hybridoma-SFM offre des densités cellulaires et une production d’IgG élevées. Les cultures dépendantes du cholestérol telles que le NS0 nécessitent l’ajout de concentré lipide cholestérol 250X.
Formulation complète, prête à l’emploi
Hybridoma-SFM est un milieu complet prêt à l’emploi. Aucune supplémentation n’est nécessaire.
Formulation exempte de sérum et contenant très peu de protéines
Hybridoma-SFM est un milieu exempt de sérum et contenant très peu de protéines facilitant la purification de votre protéine d’intérêt. Hybridoma-SFM contient un taux de protéines totales < 20 µg/ml.
Utilisation du produit
Les clients qui utilisent le milieu Gibco® Hybridoma-SFM dans un procédé de fabrication, et ayant soumis une demande à la FDA, peuvent nous demander une lettre d’autorisation pour référencer notre Drug Master File ou DMF (fichier maître des médicaments) de type II.
Fabrication et système de qualité conformes aux BPFa
Le milieu Hybridoma-SFM est fabriqué dans un site conforme aux BPFa situé à Grand Island, New York (États-Unis). Le site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485.
LV-MAX™ Production Medium (Gibco™)
Gibco LV-MAX Production Medium is a chemically defined, serum-free, protein-free medium, specially developed for high-density growth and transfection of Gibco Viral Production Cells (suspension-adapted HEK 293-derived cells) promoting high-titer lentiviral production.
• Supports growth of suspension Viral Production Cells to densities of over 1.0 x 107 cells/mL
• Enables sustained, high-level production of high-density transiently transfected cultures, achieving titer of up to 1 x 108 TU/mL (unconcentrated LVVs-GFP)
• Supports growth and transfection of Viral Production Cells in culture formats from 96-deep well blocks to 2-L flasks and bioreactors
LV-MAC Production Medium is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. The medium is not recommended for adherent cell cultures.
• Supports growth of suspension Viral Production Cells to densities of over 1.0 x 107 cells/mL
• Enables sustained, high-level production of high-density transiently transfected cultures, achieving titer of up to 1 x 108 TU/mL (unconcentrated LVVs-GFP)
• Supports growth and transfection of Viral Production Cells in culture formats from 96-deep well blocks to 2-L flasks and bioreactors
LV-MAC Production Medium is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. The medium is not recommended for adherent cell cultures.
AmnioMAX™ C-100 Complete Medium (Gibco™)
Gibco® AmnioMAX™ C-100 Complete Medium is a kit used to prepare a fully-supplemented medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures.
Gibco® AmnioMAX™ C-100 Complete Medium features:
• Easy-to-use format
• Quality and performance testing
• Unique, optimized formulation
Easy-to-use format
Gibco® AmnioMAX™ C-100 Complete Medium is a two-part kit for preparation of a complete medium that requires no additional supplementation. The complete medium can be stored at 2–8°C for up to ten days.
Quality and performance testing
Every lot of Gibco® AmnioMAX™ C-100 Complete Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Pooled primary human amniotic fluid samples are cultured for six days in Gibco® AmnioMAX™ C-100 Complete Medium before measuring the total number of colonies and total number of mitotic colonies. In addition, each lot is tested for sterility, pH and osmolality.
Unique, optimized formulation
Gibco® AmnioMAX™ C-100 Complete Medium contains Fetal Bovine Serum (FBS), gentamicin, and L-glutamine to maximize cell attachment and growth. This optimized medium also has an enhanced buffering system that provides greater pH stability during culture manipulations.
Product Use
For in vitro diagnostic use.
cGMP Manufacturing and Quality System
Gibco® AmnioMAX™ C-100 Complete Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Gibco® AmnioMAX™ C-100 Complete Medium features:
• Easy-to-use format
• Quality and performance testing
• Unique, optimized formulation
Easy-to-use format
Gibco® AmnioMAX™ C-100 Complete Medium is a two-part kit for preparation of a complete medium that requires no additional supplementation. The complete medium can be stored at 2–8°C for up to ten days.
Quality and performance testing
Every lot of Gibco® AmnioMAX™ C-100 Complete Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Pooled primary human amniotic fluid samples are cultured for six days in Gibco® AmnioMAX™ C-100 Complete Medium before measuring the total number of colonies and total number of mitotic colonies. In addition, each lot is tested for sterility, pH and osmolality.
Unique, optimized formulation
Gibco® AmnioMAX™ C-100 Complete Medium contains Fetal Bovine Serum (FBS), gentamicin, and L-glutamine to maximize cell attachment and growth. This optimized medium also has an enhanced buffering system that provides greater pH stability during culture manipulations.
Product Use
For in vitro diagnostic use.
cGMP Manufacturing and Quality System
Gibco® AmnioMAX™ C-100 Complete Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Neurobasal™ Medium (Gibco™)
Le milieu de culture Neurobasal® est un milieu basal qui satisfait les exigences particulières de la culture de cellules neuronales prénatales et embryonnaires quand il est utilisé avec le supplément GIBCO® B-27®. Le milieu de culture Neurobasal® peut être utilisé pour le développement de cellules neuronales à partir de l’hippocampe, du cortex et autres régions du cerveau. Le milieu de culture Neurobasal® permet la maintenance à long terme comme à court terme des populations homogènes de cellules neuronales sans nécessiter une couche nourricière d’astrocytes.
Ajouter vos propres suppléments
Tous les milieux de culture Neurobasal® ne contiennent ni L-glutamine, ni acide L-glutamique ni acide aspartique. Les milieux Neurobasal® doivent être supplémentés par 0,5 mM de L-glutamine ou de supplément GlutaMAX™-I et soit un supplément sans sérum (tel que le supplément B-27® soitle supplément N-2), ou avec du sérum. Pour l’étalement initial, vous devez ajouter 25 µM d’acide glutamique.
Test du contrôle qualité
Tous les milieux Neurobasal® sont testés pour vérifier leur pH, leur osmolalité, les endotoxines, et l’absence de contamination bactérienne et fongique.
Le milieu Neurobasal® contient du rouge de phénol. Si le rouge de phénol ne convient pas à vos applications, nous fournissons également un milieu Neurobasal® sans rouge de phénol.
Le milieu de culture Neurobasal® est optimisé pour les neurones prénatals et embryonnaires. Pour la mise en culture de neurones du cerveau post-natals et adultes, nous proposons le milieu A Neurobasal®.
Consultez la gamme complète de produits Neurobasal®.
Pour en savoir plus sur les produits de culture cellulaire neurale d’Invitrogen, rendez-vous sur www.invitrogen.com/neuralculture.
Usage exclusivement réservé à la recherche. Non destiné à des fins thérapeutiques ou diagnostiques humaines ou animales.
Ajouter vos propres suppléments
Tous les milieux de culture Neurobasal® ne contiennent ni L-glutamine, ni acide L-glutamique ni acide aspartique. Les milieux Neurobasal® doivent être supplémentés par 0,5 mM de L-glutamine ou de supplément GlutaMAX™-I et soit un supplément sans sérum (tel que le supplément B-27® soitle supplément N-2), ou avec du sérum. Pour l’étalement initial, vous devez ajouter 25 µM d’acide glutamique.
Test du contrôle qualité
Tous les milieux Neurobasal® sont testés pour vérifier leur pH, leur osmolalité, les endotoxines, et l’absence de contamination bactérienne et fongique.
Le milieu Neurobasal® contient du rouge de phénol. Si le rouge de phénol ne convient pas à vos applications, nous fournissons également un milieu Neurobasal® sans rouge de phénol.
Le milieu de culture Neurobasal® est optimisé pour les neurones prénatals et embryonnaires. Pour la mise en culture de neurones du cerveau post-natals et adultes, nous proposons le milieu A Neurobasal®.
Consultez la gamme complète de produits Neurobasal®.
Pour en savoir plus sur les produits de culture cellulaire neurale d’Invitrogen, rendez-vous sur www.invitrogen.com/neuralculture.
Usage exclusivement réservé à la recherche. Non destiné à des fins thérapeutiques ou diagnostiques humaines ou animales.
Sf-900™ Medium (1.3X) (Gibco™)
Sf-900™ SFM is a complete serum-free, low protein insect cell culture medium developed for the convenient and reproducible formation of 1% agarose overlays used for plaque assays of baculovirus in Spodoptera frugiperda (Sf9 and Sf21) cells. When used with Gibco® 4% Agarose Gel, Sf900™ SFM (1.3x) allows for convenient formation of a 1% overlay with correct 1x final osmolality and nutrition required for insect cell growth during the plaque assay.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
cGMP Manufacturing and Quality System
Gibco® Sf-900 II SFM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
cGMP Manufacturing and Quality System
Gibco® Sf-900 II SFM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
VP-SFM AGT™ Medium (Gibco™)
VP-SFM AGT™ is a serum-free, ultra-low protein medium in an easy-to-use granular format. It is designed for the growth of VERO cells for virus production and for the growth of COS-7, MDCK, BHK-21 and Hep-2 cells for recombinant protein production.
Advanced Granulation Technology = AGT
Advanced Granulation Technology = AGT
Sf-900™ III SFM (Gibco™)
Sf-900™ III SFM is a low-hydrolysate, serum-free, protein-free, animal origin–free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for recombinant gene expression using the baculovirus and stable insect expression systems. This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Features of Gibco® Sf-900™ III SFM:
• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, animal origin–free, ready-to-use formulation
• Improved lot-to-lot consistency
Superior long-term, high-density growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ III SFM achieve maximum cell densities of 10 to 14 × 106 cells/mL, a significant improvement over Sf-900™ II SFM and other commercially available serum-free media (see figure).
Optimized for recombinant protein production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ III SFM is an improved serum-free, protein-free, animal origin–free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect viruses and recombinant proteins.
Serum-free, protein-free, animal origin–free, ready-to-use formulation
Gibco® Sf-900™ III SFM is a serum-free, protein-free, animal origin–free medium that allows for much easier purification of your protein of interest. Sf-900™ III SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media should be sequentially adapted into Sf-900™ III SFM (see product manual for details).
Improved lot-to-lot consistency
Gibco® Sf-900™ III SFM contains reduced hydrolysate concentrations, improving lot-to-lot consistency over Sf-900™ II SFM (see figure).
cGMP manufacturing and quality system
Gibco® Sf-900 II SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, animal origin–free, ready-to-use formulation
• Improved lot-to-lot consistency
Superior long-term, high-density growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ III SFM achieve maximum cell densities of 10 to 14 × 106 cells/mL, a significant improvement over Sf-900™ II SFM and other commercially available serum-free media (see figure).
Optimized for recombinant protein production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ III SFM is an improved serum-free, protein-free, animal origin–free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect viruses and recombinant proteins.
Serum-free, protein-free, animal origin–free, ready-to-use formulation
Gibco® Sf-900™ III SFM is a serum-free, protein-free, animal origin–free medium that allows for much easier purification of your protein of interest. Sf-900™ III SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media should be sequentially adapted into Sf-900™ III SFM (see product manual for details).
Improved lot-to-lot consistency
Gibco® Sf-900™ III SFM contains reduced hydrolysate concentrations, improving lot-to-lot consistency over Sf-900™ II SFM (see figure).
cGMP manufacturing and quality system
Gibco® Sf-900 II SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Grace's Insect Medium, supplemented (Gibco™)
Grace's Insect Medium, Supplemented, was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. Grace's Supplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21.
Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). We offer a variety of Gibco® media options for a range of insect cell culture applications.
Formulation
This Grace's Insect Medium contains lactalbumin hydrolysate, yeastolate, L-glutamine, and sodium bicarbonate. The complete formulation is available. Grace's Supplemented Insect Medium contains lactalbumin hydrolysate and yeastolate not commonly found in the unsupplemented version.
Dual site cGMP manufacturing and quality system
Gibco® Grace's Supplemented Insect Medium is manufactured at a cGMP-compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® Grace's Supplemented Insect Medium made in our Grand Island facility (11605-094). This facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and 9001 standards.
Grace's Supplemented Insect Medium contains lactalbumin hydrolysate and yeastolate, and is commonly supplemented with 10% Fetal Bovine Serum (FBS) before use. Grace's Supplemented Insect Medium requires ambient CO2 in order to maintain physiological pH.
Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). We offer a variety of Gibco® media options for a range of insect cell culture applications.
Formulation
This Grace's Insect Medium contains lactalbumin hydrolysate, yeastolate, L-glutamine, and sodium bicarbonate. The complete formulation is available. Grace's Supplemented Insect Medium contains lactalbumin hydrolysate and yeastolate not commonly found in the unsupplemented version.
Dual site cGMP manufacturing and quality system
Gibco® Grace's Supplemented Insect Medium is manufactured at a cGMP-compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® Grace's Supplemented Insect Medium made in our Grand Island facility (11605-094). This facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and 9001 standards.
Grace's Supplemented Insect Medium contains lactalbumin hydrolysate and yeastolate, and is commonly supplemented with 10% Fetal Bovine Serum (FBS) before use. Grace's Supplemented Insect Medium requires ambient CO2 in order to maintain physiological pH.
FreeStyle™ F17 Expression Medium (Gibco™)
FreeStyle™ F17 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation. This is a multi-platform medium compatible with a range of transient expression systems. The addition of 4–8 mM L-glutamine or GlutaMAX™ supplement is required before use. Gibco® FreeStyle™ F17 Expression Medium features:
• Little or no required adaptation
• Ability to support suspension growth and transfection of 293-F and CHO cells
• Animal origin–free, protein-free, and chemically defined formulation
• Scalability in spinner flasks and bioreactors
Little or no required adaptation
Gibco® 293-F, 293-H, FreeStyle™ 293-F, and CHO-S cells in suspension can be cultured directly into Gibco® FreeStyle™ F17 Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ F17 Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.
Ability to support suspension growth and transfection of 293-F and CHO cells
Gibco® FreeStyle™ F17 Medium provides equal or better performance compared to Gibco® FreeStyle™ 293 Expression Medium and Gibco® FreeStyle™ CHO Medium. Gibco® FreeStyle™ F17 Medium supports 293-F cells at a peak density of 6–7 × 106 viable cells per mL, and supports CHO-S cells at a peak density of 8–9 × 106 viable cells per mL. Cells cultured in Gibco® FreeStyle™ F17 can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin™ and Freestyle™ MAX Reagent. Protein expression typically exceeds 75–150 µg/mL IgG in 293-F cells.
Animal origin–free, protein-free, and chemically defined formulation
Gibco® FreeStyle™ F17 Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.
Scalability in spinner flasks and bioreactors
Protein production using Gibco® FreeStyle™ F17 Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ F17 Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.
cGMP manufacturing and quality system
Gibco® FreeStyle™ F17 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
• Little or no required adaptation
• Ability to support suspension growth and transfection of 293-F and CHO cells
• Animal origin–free, protein-free, and chemically defined formulation
• Scalability in spinner flasks and bioreactors
Little or no required adaptation
Gibco® 293-F, 293-H, FreeStyle™ 293-F, and CHO-S cells in suspension can be cultured directly into Gibco® FreeStyle™ F17 Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ F17 Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.
Ability to support suspension growth and transfection of 293-F and CHO cells
Gibco® FreeStyle™ F17 Medium provides equal or better performance compared to Gibco® FreeStyle™ 293 Expression Medium and Gibco® FreeStyle™ CHO Medium. Gibco® FreeStyle™ F17 Medium supports 293-F cells at a peak density of 6–7 × 106 viable cells per mL, and supports CHO-S cells at a peak density of 8–9 × 106 viable cells per mL. Cells cultured in Gibco® FreeStyle™ F17 can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin™ and Freestyle™ MAX Reagent. Protein expression typically exceeds 75–150 µg/mL IgG in 293-F cells.
Animal origin–free, protein-free, and chemically defined formulation
Gibco® FreeStyle™ F17 Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.
Scalability in spinner flasks and bioreactors
Protein production using Gibco® FreeStyle™ F17 Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ F17 Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.
cGMP manufacturing and quality system
Gibco® FreeStyle™ F17 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
CD FortiCHO™ Medium (Gibco™)
Le milieu de culture CD FortiCHO™ est spécifiquement conçu pour offrir la meilleure des performances de culture par lot et par lot nourricier et le meilleur rendement avec des cellules CHO recombinantes dans un environnement chimiquement défini. La formulation exempte de protéines et exempte de composants d’origine animale garantit la constance et contribue à réduire le besoin en analyse d’agents fortuits. Avec le milieu CD FortiCHO™, les cultures cellulaires durent plus de 2 jours de plus que les alternatives de la concurrence, ce qui signifie une baisse de la concentration des HCP (protéines hôtes) pour l’élimination dans la purification en aval.
Le milieu de culture CD FortiCHO™ est fortifié pour prendre en charge une croissance des cellules CHO recombinantes et une expression des protéines maximales dans les cultures par lot en suspension, offrant 120 % de plus de croissance et de production que certains milieux de la concurrence avec une simple supplémentation en glucose. Le milieu de culture CD FortiCHO™ peut fournir jusqu’à 75 % de croissance et de production de lots nourriciers en plus par rapport à la culture de lots nourriciers au glucose uniquement pour certaines lignées cellulaires et applications lorsqu’il est utilisé avec le supplément nutriant CD EfficientFeed™.
Quand utiliser le milieu CD FortiCHO™
• La lignée cellulaire utilisée est une lignée cellulaire CHO K1, GS CHO ou CHO-S™ transfectée.
• Des densités cellulaires maximum de culture de lots et des titres protéiniques sont nécessaires.
• Pour optimiser les titres, vous disposez de suffisamment de temps pour adapter les cellules à un nouveau milieu.
• La santé cellulaire au moment de la récolte est l’une des priorités essentielles pour le traitement en aval
Quand envisager le recours à un autre milieu de culture Gibco®
Envisagez le recours à un milieu de culture CD OptiCHO™ dans les cas suivants :
• Vous utilisez une lignée cellulaire CHO transfectée autre que CHO K1, GS CHO ou CHO-S™
• Vous intégrerez un processus d’optimisation des stratégies nourricières à vos travaux de sélection d’un milieu de culture de base en vue d’optimiser la productivité
• Votre lignée cellulaire CHO est “délicate”, c’est-à-dire qu’elle a des difficultés à se développer ou à s’adapter
Envisagez le recours à un milieu de culture CD CHO dans les cas suivants :
• Un milieu de culture CD CHO est recommandé pour vos cellules GS CHO
• Vos cellules ont été cultivées correctement dans un milieu CD CHO dans le passé et votre processus doit bientôt faire l’objet d’un archivage réglementaire (DMF disponible)
Envisagez le recours à CD DG44 dans les cas suivants :
• Vous utilisez des cellules ovariennes de hamster (CHO) DG44 ou DXB11 parentales (dhfr-)
Formulations de milieu sec
Pour un accès anticipé au milieu CD FortiCHO™ AGT™, veuillez contacter les services techniques de Gibco®.
Créez un milieu sur mesure adapté à vos besoins
Nous proposons des formats spécialisés, des conditionnements et des niveaux de qualité adaptés à vos besoins, qu’il s’agisse d’une expérience sur paillasse ou d’un processus de bioproduction à grande échelle. Les services personnalisés de milieux Gibco® conformes aux BPFa vous permettent de tirer profit de toutes les capacités de production et des nombreuses années d’expérience de nos spécialistes en matière de recherche et développement et d’applications de bioproduction.
Le milieu de culture CD FortiCHO™ est fortifié pour prendre en charge une croissance des cellules CHO recombinantes et une expression des protéines maximales dans les cultures par lot en suspension, offrant 120 % de plus de croissance et de production que certains milieux de la concurrence avec une simple supplémentation en glucose. Le milieu de culture CD FortiCHO™ peut fournir jusqu’à 75 % de croissance et de production de lots nourriciers en plus par rapport à la culture de lots nourriciers au glucose uniquement pour certaines lignées cellulaires et applications lorsqu’il est utilisé avec le supplément nutriant CD EfficientFeed™.
Quand utiliser le milieu CD FortiCHO™
• La lignée cellulaire utilisée est une lignée cellulaire CHO K1, GS CHO ou CHO-S™ transfectée.
• Des densités cellulaires maximum de culture de lots et des titres protéiniques sont nécessaires.
• Pour optimiser les titres, vous disposez de suffisamment de temps pour adapter les cellules à un nouveau milieu.
• La santé cellulaire au moment de la récolte est l’une des priorités essentielles pour le traitement en aval
Quand envisager le recours à un autre milieu de culture Gibco®
Envisagez le recours à un milieu de culture CD OptiCHO™ dans les cas suivants :
• Vous utilisez une lignée cellulaire CHO transfectée autre que CHO K1, GS CHO ou CHO-S™
• Vous intégrerez un processus d’optimisation des stratégies nourricières à vos travaux de sélection d’un milieu de culture de base en vue d’optimiser la productivité
• Votre lignée cellulaire CHO est “délicate”, c’est-à-dire qu’elle a des difficultés à se développer ou à s’adapter
Envisagez le recours à un milieu de culture CD CHO dans les cas suivants :
• Un milieu de culture CD CHO est recommandé pour vos cellules GS CHO
• Vos cellules ont été cultivées correctement dans un milieu CD CHO dans le passé et votre processus doit bientôt faire l’objet d’un archivage réglementaire (DMF disponible)
Envisagez le recours à CD DG44 dans les cas suivants :
• Vous utilisez des cellules ovariennes de hamster (CHO) DG44 ou DXB11 parentales (dhfr-)
Formulations de milieu sec
Pour un accès anticipé au milieu CD FortiCHO™ AGT™, veuillez contacter les services techniques de Gibco®.
Créez un milieu sur mesure adapté à vos besoins
Nous proposons des formats spécialisés, des conditionnements et des niveaux de qualité adaptés à vos besoins, qu’il s’agisse d’une expérience sur paillasse ou d’un processus de bioproduction à grande échelle. Les services personnalisés de milieux Gibco® conformes aux BPFa vous permettent de tirer profit de toutes les capacités de production et des nombreuses années d’expérience de nos spécialistes en matière de recherche et développement et d’applications de bioproduction.
