Shop All Opti-MEM I
Opti-MEM™ Reduced Serum Medium, GlutaMAX™ Supplement (Gibco™)
Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM® I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM® I modifications for a range of cell culture applications.
This Opti-MEM® I is modified as follows:
The complete formulation is confidential. For more information, please contact Technical Services.
Using Opti-MEM® I Medium
Opti-MEM® I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM® I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
Opti-MEM® I is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Opti-MEM® I product made in our Scotland facility (51985-042). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.
This Opti-MEM® I is modified as follows:
| With |
| • GlutaMAX™ |
| • Phenol Red |
The complete formulation is confidential. For more information, please contact Technical Services.
Using Opti-MEM® I Medium
Opti-MEM® I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM® I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
Opti-MEM® I is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Opti-MEM® I product made in our Scotland facility (51985-042). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.
Opti-MEM™ I Reduced Serum Medium, GlutaMAX™ Supplement (Gibco™)
Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications.
The complete formulation is confidential. For more information, please contact Technical Services.
Gibco® Opti-MEM® is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Gibco® Opti-MEM® with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Opti-MEM® is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® Opti-MEM® product made in our Grand Island facility (31985-070). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Gibco® Opti-MEM® uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
This Opti-MEM® is modified as follows:
| With |
| • GlutaMAX™ |
| • Phenol Red |
The complete formulation is confidential. For more information, please contact Technical Services.
Gibco® Opti-MEM® is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Gibco® Opti-MEM® with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Opti-MEM® is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® Opti-MEM® product made in our Grand Island facility (31985-070). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Gibco® Opti-MEM® uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Opti-MEM™ I Reduced Serum Medium (Gibco™)
Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications.
The complete formulation is confidential. For more information, please contact Technical Services.
Gibco® Opti-MEM® is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Opti-MEM® is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® Opti-MEM® product made in our Grand Island facility (31985-070). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Gibco® Opti-MEM® uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
This Opti-MEM® is modified as follows:
| With |
| • L-glutamine |
| • Phenol Red |
The complete formulation is confidential. For more information, please contact Technical Services.
Gibco® Opti-MEM® is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Opti-MEM® is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® Opti-MEM® product made in our Grand Island facility (31985-070). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Gibco® Opti-MEM® uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Opti-MEM™ I Reduced Serum Medium, no phenol red (Gibco™)
Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in cell growth rate or morphology. Opti-MEM® I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM® I Reduced-Serum Medium modifications for a range of cell culture applications.
This Opti-MEM® is modified as follows:
The complete formulation is confidential. For more information, please contact Technical Services.
Using Opti-MEM® Reduced-Serum Medium
Opti-MEM® I medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM® I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
For supply chain continuity, we manufacture Opti-MEM® Reduced-Serum Medium at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
This Opti-MEM® is modified as follows:
| With | Without |
| • L-glutamine | • Phenol Red |
The complete formulation is confidential. For more information, please contact Technical Services.
Using Opti-MEM® Reduced-Serum Medium
Opti-MEM® I medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM® I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
For supply chain continuity, we manufacture Opti-MEM® Reduced-Serum Medium at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
Opti-MEM™ I Reduced Serum Medium (Gibco™)
Le milieu de culture à réduction de sérum Opti-MEM® I est un milieu essentiel minimal (MEM) amélioré qui permet une réduction de la supplémentation en sérum de veau fœtal d’au moins 50 % sans modification du taux de croissance ni de la morphologie. Le milieu de culture Opti-MEM® I est également recommandé avec l’utilisation des réactifs de transfection des lipides cationiques, tels que le réactif Lipofectamine™. Le milieu de culture Opti-MEM® I peut être utilisé avec une variété de cellules de mammifère en suspension et adhérentes, notamment les cellules Sp2, AE-1, CHO, BHK-21, HEK, et les fibroblastes primaires. Nous proposons une variété de modifications du milieu Opti-MEM® I pour une gamme d’applications de culture cellulaire.
Ce milieu Opti-MEM® I est modifié comme suit :
La formulation complète est confidentielle. Pour plus d’informations, veuillez contacter nos services techniques.
Utilisation du milieu de culture Opti-MEM® I
Le milieu de culture à réduction de sérum Opti-MEM® I est un milieu unique qui contient insuline, transferrine, hypoxanthine, thymidine et des éléments traces. Ces composants complémentaires permettent une réduction de la supplémentation en sérum d’au moins 50 %. Le milieu de culture Opti-MEM® I utilise un système de tampon au bicarbonate de sodium (2,4 g/L), et par conséquent, nécessite un environnement contenant 5 à 10 % de CO2 pour maintenir le pH physiologique.
Fabrication et système de qualité conformes aux BPFa
Le milieu Opti-MEM® I est fabriqué sur un site conforme aux BPFa et situé à Grand Island, New York (États-Unis). Le site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485. Pour la continuité de la chaîne d’approvisionnement, nous proposons un produit identique au milieu Opti-MEM® I fabriqué dans notre installation située en Écosse (51985-042). Cette installation est également homologuée par la FDA comme fabricant de dispositifs médicaux et elle est certifiée ISO 13485.
Ce milieu Opti-MEM® I est modifié comme suit :
| Avec |
| • L-glutamine |
| • Rouge de phénol |
La formulation complète est confidentielle. Pour plus d’informations, veuillez contacter nos services techniques.
Utilisation du milieu de culture Opti-MEM® I
Le milieu de culture à réduction de sérum Opti-MEM® I est un milieu unique qui contient insuline, transferrine, hypoxanthine, thymidine et des éléments traces. Ces composants complémentaires permettent une réduction de la supplémentation en sérum d’au moins 50 %. Le milieu de culture Opti-MEM® I utilise un système de tampon au bicarbonate de sodium (2,4 g/L), et par conséquent, nécessite un environnement contenant 5 à 10 % de CO2 pour maintenir le pH physiologique.
Fabrication et système de qualité conformes aux BPFa
Le milieu Opti-MEM® I est fabriqué sur un site conforme aux BPFa et situé à Grand Island, New York (États-Unis). Le site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485. Pour la continuité de la chaîne d’approvisionnement, nous proposons un produit identique au milieu Opti-MEM® I fabriqué dans notre installation située en Écosse (51985-042). Cette installation est également homologuée par la FDA comme fabricant de dispositifs médicaux et elle est certifiée ISO 13485.
Opti-MEM™ Reduced Serum Medium, powder (Gibco™)
Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications.
The complete formulation is confidential. For more information, please contact Technical Services.
Gibco® Opti-MEM® is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco® Opti-MEM® at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
Gibco® Opti-MEM® uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation (see protocol for details).
This Opti-MEM® is modified as follows:
| With |
| • L-glutamine |
| • Phenol Red |
The complete formulation is confidential. For more information, please contact Technical Services.
Gibco® Opti-MEM® is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco® Opti-MEM® at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
Gibco® Opti-MEM® uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation (see protocol for details).
