Shop All Williams' Media E
William's E Medium, GlutaMAX™ Supplement (Gibco™)
William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco® William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.
The complete formulation is available.
Gibco® William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate. Gibco® William's E Medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing
Gibco® William's E Medium is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
This William's E Medium is modified as follows:
| With | Without |
| • Phenol Red | • HEPES |
| • GlutaMAX™ |
The complete formulation is available.
Gibco® William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate. Gibco® William's E Medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing
Gibco® William's E Medium is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
William's E Medium, no glutamine (Gibco™)
William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. This media can also be used for growing hepatocyte cells of different species, such as human HepaRG™ cells.
The complete formulation is available.
Gibco® William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.
Dual-Site cGMP Manufacturing
Gibco® William's E Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® William's E Medium product made in our Scotland facility (22551-022). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
This William's E Medium is modified as follows:
| With | Without |
| • Phenol Red | • L-glutamine |
| • HEPES |
The complete formulation is available.
Gibco® William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.
Dual-Site cGMP Manufacturing
Gibco® William's E Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® William's E Medium product made in our Scotland facility (22551-022). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
William's E Medium, no phenol red (Gibco™)
Le milieu E de William à supplémentation modérée en sérum, a été initialement élaboré par Williams et Gunn pour les cultures cellulaires de longue durée de cellules épithéliales de foie de rat adulte. Le milieu E de William Gibco® permet également de mettre en culture des hépatocytes de plusieurs espèces comme les cellules HepaRG™ d’origine humaine.
La formulation complète est disponible.
Le milieu E de William Gibco® est le résultat d’une modification de milieux précédents qui ont été enrichis en amino-acides avec une teneur en glucose double. Le milieu E de William contient des ingrédients uniques, notamment les éléments zinc, fer, manganèse, ainsi que des amino-acides non indispensables, l’agent de réduction glutathion et un lipide, le linoléate de méthyle.
Fabrication suivant les BPFa sur deux sites
Afin de garantir la continuité de la chaîne d’approvisionnement, nous manufacturons le milieu E de William Gibco® dans deux installations différentes : une située à Grand Island, État de New York, États-Unis, l’autre en Écosse, au Royaume-Uni. Les deux sites sont conformes aux BPFa, certifiés ISO 13485 et homologués par la FDA comme fabricants de dispositifs médicaux.
Le milieu E de William ne contient pas de protéines ni de facteurs de croissance. Par conséquent, le milieu E de William nécessite une supplémentation, en général avec 5 à 10 % de sérum de veau fœtal (SVF). Le milieu E de William utilise un système de tampon au bicarbonate de sodium (2,2 g/l), et par conséquent nécessite un environnement contenant 5 à 10 % de CO2 pour maintenir le pH physiologique.
Ce milieu E de William est modifié comme suit :
| Sans |
| • Rouge de phénol |
| • L-glutamine |
| • HEPES |
La formulation complète est disponible.
Le milieu E de William Gibco® est le résultat d’une modification de milieux précédents qui ont été enrichis en amino-acides avec une teneur en glucose double. Le milieu E de William contient des ingrédients uniques, notamment les éléments zinc, fer, manganèse, ainsi que des amino-acides non indispensables, l’agent de réduction glutathion et un lipide, le linoléate de méthyle.
Fabrication suivant les BPFa sur deux sites
Afin de garantir la continuité de la chaîne d’approvisionnement, nous manufacturons le milieu E de William Gibco® dans deux installations différentes : une située à Grand Island, État de New York, États-Unis, l’autre en Écosse, au Royaume-Uni. Les deux sites sont conformes aux BPFa, certifiés ISO 13485 et homologués par la FDA comme fabricants de dispositifs médicaux.
Le milieu E de William ne contient pas de protéines ni de facteurs de croissance. Par conséquent, le milieu E de William nécessite une supplémentation, en général avec 5 à 10 % de sérum de veau fœtal (SVF). Le milieu E de William utilise un système de tampon au bicarbonate de sodium (2,2 g/l), et par conséquent nécessite un environnement contenant 5 à 10 % de CO2 pour maintenir le pH physiologique.
William's E Medium, no glutamine (Gibco™)
William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco® William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.
The complete formulation is available.
Gibco® William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
Dual-Site cGMP Manufacturing
Gibco® William's E Medium is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® William's E Medium product made in our Grand Island facility (12551-032). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
This William's E Medium is modified as follows:
| With | Without |
| • Phenol Red | • L-glutamine |
| • HEPES |
The complete formulation is available.
Gibco® William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
Dual-Site cGMP Manufacturing
Gibco® William's E Medium is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® William's E Medium product made in our Grand Island facility (12551-032). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Hepatocyte Wash Medium (Gibco™)
Hepatocyte Wash Medium is an enriched Williams' Medium E for the reduction of cell membrane leakage, ease of centrifugation and high viability.
