Shop All Media 199
Medium 199, Hanks' Balanced Salts, powder (Gibco™)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. This Medium 199 formulation contains Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing
Gibco® Medium 199 is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). This Medium 199 formulation does not require a CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
This Medium 199 is modified as follows:
| With | Without |
| • Hanks' salts | • Sodium Bicarbonate |
| • L-glutamine | |
| • HEPES | |
| • Phenol Red |
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. This Medium 199 formulation contains Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing
Gibco® Medium 199 is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). This Medium 199 formulation does not require a CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Medium 199, powder (Gibco™)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® Medium 199 product made in our Grand Island facility (31100-035). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation (see protocol for details).
This Medium 199 is modified as follows:
| With | Without |
| • Phenol Red | • Sodium Bicarbonate |
| • L-glutamine | • HEPES |
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® Medium 199 product made in our Grand Island facility (31100-035). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation (see protocol for details).
Medium 199, no phenol red (Gibco™)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
This M199 is modified as follows:
| With | Without |
| • L-glutamine | • Phenol Red |
| • HEPES |
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Medium 199, Hanks' Balanced Salts (Gibco™)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. This Medium 199 formulation contains Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® Medium 199 product made in our Scotland facility (22350-029). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. Therefore, Medium 199 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). This Medium 199 formulation does not require a CO2 environment to maintain physiological pH.
This Medium 199 is modified as follows:
| With |
| • Hanks' salts |
| • L-glutamine |
| • HEPES |
| • Phenol Red |
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. This Medium 199 formulation contains Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® Medium 199 product made in our Scotland facility (22350-029). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. Therefore, Medium 199 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). This Medium 199 formulation does not require a CO2 environment to maintain physiological pH.
Medium 199, low sodium bicarbonate (Gibco™)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. This Medium 199 uses a sodium bicarbonate buffer system (1.25 g/L) and therefore requires a 3-5% CO2 environment to maintain physiological pH.
This Medium 199 is modified as follows:
| With | |
| • L-glutamine | |
| • Phenol Red | |
| • Sodium Bicarbonate |
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. This Medium 199 uses a sodium bicarbonate buffer system (1.25 g/L) and therefore requires a 3-5% CO2 environment to maintain physiological pH.
Medium 199, HEPES (Gibco™)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® Medium 199 product made in our Grand Island facility (12340-030). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
This Medium 199 is modified as follows:
| With |
| • L-glutamine |
| • Phenol Red |
| • HEPES |
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® Medium 199 product made in our Grand Island facility (12340-030). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Medium 199, Earle's Salts, powder (Gibco™)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
In Vitro Diagnostic
Gibco® Medium 199 is manufactured in compliance with the requirements of ISO 13485: 2003 for the design, development, manufacture, and distribution of liquid and powder tissue culture media, sera, and reagents for cell culture research, industrial bioprocessing, and related markets.
Dual-Site cGMP Manufacturing
Gibco® Medium 199 is manufactured in a cGMP New York facility that is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 . For supply chain continuity, we offer an identical Gibco® Medium 199 product made in the Scotland facility (31100-019). This second cGMP manufacturing location is also registered with the FDA as a medical device manufacturer and is certified to ISO 13485. Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
This M199 is modified as follows:
| With | Without |
| • L-glutamine | • HEPES |
| • Phenol Red | • Sodium bicarbonate |
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
In Vitro Diagnostic
Gibco® Medium 199 is manufactured in compliance with the requirements of ISO 13485: 2003 for the design, development, manufacture, and distribution of liquid and powder tissue culture media, sera, and reagents for cell culture research, industrial bioprocessing, and related markets.
Dual-Site cGMP Manufacturing
Gibco® Medium 199 is manufactured in a cGMP New York facility that is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 . For supply chain continuity, we offer an identical Gibco® Medium 199 product made in the Scotland facility (31100-019). This second cGMP manufacturing location is also registered with the FDA as a medical device manufacturer and is certified to ISO 13485. Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Medium 199, GlutaMAX™ Supplement (Gibco™)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
This Medium 199 is modified as follows:
| With | Without |
| • GlutaMAX™ | • HEPES |
| • Phenol Red |
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Medium 199, Earle's Salts (Gibco™)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® Medium 199 product made in our Grand Island facility (11150-067). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
This Medium 199 is modified as follows:
| With | Without |
| • L-glutamine | • HEPES |
| • Phenol Red |
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® Medium 199 product made in our Grand Island facility (11150-067). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Medium 199, Earle's Salts (10X) (Gibco™)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® Medium 199 product made in our Grand Island facility (11825-015). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Concentrated forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
This Medium 199 is modified as follows:
| With | Without |
| • Phenol Red | • HEPES |
| • L-glutamine | • Sodium Bicarbonate |
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® Medium 199 product made in our Grand Island facility (11825-015). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Concentrated forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Medium 199 (2X) (Gibco™)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This Medium 199 is made with Earle's salts.
Product Use
For in vitro diagnostic use.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
This Medium 199 is modified as follows:
| With | Without |
| L-glutamine | HEPES |
| Phenol Red |
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This Medium 199 is made with Earle's salts.
Product Use
For in vitro diagnostic use.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Medium 199 (10X) (Gibco™)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® Medium 199 product made in our Scotland facility (21180-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Concentrated forms of Gibco® Medium 199 require sodium bicarbonate addition, pH adjustment, and filtration at the time of preparation (see protocol for details).
This M199 is modified as follows:
| With | Without |
| • Phenol Red | • L-glutamine |
| • HEPES | |
| • Sodium Bicarbonate |
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® Medium 199 product made in our Scotland facility (21180-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Concentrated forms of Gibco® Medium 199 require sodium bicarbonate addition, pH adjustment, and filtration at the time of preparation (see protocol for details).
Medium 199, Hanks' Balanced Salts (Gibco™)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. This Medium 199 formulation contains Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
Dual-Site cGMP Manufacturing
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® Medium 199 product made in our Grand Island facility (12350-039). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Medium 199 contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). This Medium 199 formulation does not require a CO2 environment to maintain physiological pH.
This Medium 199 is modified as follows:
| With |
| • Hanks' salts |
| • L-glutamine |
| • HEPES |
| • Phenol Red |
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. This Medium 199 formulation contains Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
Dual-Site cGMP Manufacturing
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® Medium 199 product made in our Grand Island facility (12350-039). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Medium 199 contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). This Medium 199 formulation does not require a CO2 environment to maintain physiological pH.
Medium 199, Earle's Salts (Gibco™)
Le milieu 199 a été initialement développé pour des études nutritionnelles de fibroblastes d’embryons de poulets. Il possède une large applicabilité en termes d’espèces, en particulier pour la culture de cellules non transformées. Le milieu 199 est largement utilisé en virologie, production de vaccins et culture in vitro d’explants primaires d’épithélium pancréatique de souris et de tissus cristalliniens de rats. Nous offrons diverses modifications de milieu Gibco® 199 pour un éventail d’applications de culture cellulaire. Trouvez la formulation la mieux adaptée à l’aide de l’outil de sélection de milieux de culture.
La formulation complète est disponible.
Par rapport à d’autres milieux de base, le milieu 199 contient des composants uniques, dont de l’adénine, de l’adénosine, de l’hypoxanthine, de la thymine et des vitamines supplémentaires. Le milieu 199 est disponible avec des sels de Earle pour utilisation dans un incubateur à CO2, ou avec des sels de Hanks pour utilisation sans CO2.
Utilisation prévue du produit
Utilisé pour diagnostics in vitro. MISE EN GARDE : N’est pas utilisé à des fins thérapeutiques humaines ou animales. Les usages autres que ceux prévus peuvent constituer une violation de la législation locale.
Fabrication et système de qualité conformes aux BPFa
Le milieu Gibco® 199 est fabriqué sur un site conforme aux BPFa et situé à Grand Island, New York (États-Unis). Le site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485. Pour assurer la continuité de la chaîne d’approvisionnement, nous offrons un produit identique au milieu Gibco® 199 fabriqué sur notre site situé en Écosse (31150-022). Ce site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485.
Le milieu 199 ne contient ni protéines, ni lipides ni facteurs de croissance. Le milieu 199 utilise un système tampon au bicarbonate de sodium (2,2 g/l) et, par conséquent, nécessite un environnement contenant de 5 à 10 % de CO2 afin de conserver un pH physiologique.
Ce milieu M199 est modifié comme suit :
| Avec | Sans |
| • Rouge de phénol | • HEPES |
| • L-glutamine |
La formulation complète est disponible.
Par rapport à d’autres milieux de base, le milieu 199 contient des composants uniques, dont de l’adénine, de l’adénosine, de l’hypoxanthine, de la thymine et des vitamines supplémentaires. Le milieu 199 est disponible avec des sels de Earle pour utilisation dans un incubateur à CO2, ou avec des sels de Hanks pour utilisation sans CO2.
Utilisation prévue du produit
Utilisé pour diagnostics in vitro. MISE EN GARDE : N’est pas utilisé à des fins thérapeutiques humaines ou animales. Les usages autres que ceux prévus peuvent constituer une violation de la législation locale.
Fabrication et système de qualité conformes aux BPFa
Le milieu Gibco® 199 est fabriqué sur un site conforme aux BPFa et situé à Grand Island, New York (États-Unis). Le site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485. Pour assurer la continuité de la chaîne d’approvisionnement, nous offrons un produit identique au milieu Gibco® 199 fabriqué sur notre site situé en Écosse (31150-022). Ce site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485.
Le milieu 199 ne contient ni protéines, ni lipides ni facteurs de croissance. Le milieu 199 utilise un système tampon au bicarbonate de sodium (2,2 g/l) et, par conséquent, nécessite un environnement contenant de 5 à 10 % de CO2 afin de conserver un pH physiologique.
Medium 199, HEPES (Gibco™)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® Medium 199 product made in our Scotland facility (22340-020). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
This M199 is modified as follows:
| With |
| • Phenol Red |
| • HEPES |
| • L-glutamine |
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® Medium 199 product made in our Scotland facility (22340-020). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
