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Pierce™ Protein Concentrator PES, 10K MWCO, 2-6 mL (Thermo Scientific™)
Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 10K and can be used for processing sample volumes ranging from 2 to 6 mL.
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.
Pierce™ Centrifuge Columns, 0.8 mL (Thermo Scientific™)
Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µL to 10 mL of an affinity support for purification.
Column details:
• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 800 µL
• Resin volume: 40-400 µL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard microcentrifuge tubes
• Caps: O-ring screw-top cap
• Twist-off bottom
Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.
Applications:
• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange
Related Products
Pierce™ Centrifuge Columns, 2 mL
Pierce™ Centrifuge Columns, 5 mL
Pierce™ Centrifuge Columns, 10 mL
Column Extenders for Pierce™ Centrifuge Columns
Column details:
• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 800 µL
• Resin volume: 40-400 µL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard microcentrifuge tubes
• Caps: O-ring screw-top cap
• Twist-off bottom
Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.
Applications:
• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange
Related Products
Pierce™ Centrifuge Columns, 2 mL
Pierce™ Centrifuge Columns, 5 mL
Pierce™ Centrifuge Columns, 10 mL
Column Extenders for Pierce™ Centrifuge Columns
NucAway™ Spin Columns (Invitrogen™)
Ambion® NucAway™ Spin Columns are for the fast removal of unincorporated nucleotides and salts from probes after probe synthesis reactions. 30 columns are provided.
• Fast, efficient removal of unincorporated nucleotides and salts after probe synthesis
• RNase- and DNase-free
• Replaces more time- consuming purification methods To use, simply rehydrate the column, centrifuge to remove the interstitial fluid, add the probe to the top of the column, and centrifuge again to elute the probe.
• Fast, efficient removal of unincorporated nucleotides and salts after probe synthesis
• RNase- and DNase-free
• Replaces more time- consuming purification methods To use, simply rehydrate the column, centrifuge to remove the interstitial fluid, add the probe to the top of the column, and centrifuge again to elute the probe.
Pierce™ Protein Concentrator PES, 5K MWCO, 20-100 mL (Thermo Scientific™)
Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 5K and can be used for processing sample volumes ranging from 20 to 100 mL.
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.
Pierce™ Protein Concentrators PES, 3K MWCO, 0.5 mL (Thermo Scientific™)
Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting and buffer exchange of biological samples. This product has a molecular-weight cutoff of 3K, and can be used for processing sample volumes up to 0.5 mL.
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 10 minutes or less
• Excellent protein recovery—unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions
• Instrument-compatible—use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning
• Versatile—easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low non-specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. Sample concentration of 10- to 30-fold can be achieved within 10 minutes.
Applications:
• Protein concentration with tissue culture media, antiserum or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns
• Separating large molecules from small molecules
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.05 mg/mL.
Pierce Protein Concentrators PES allow accurate control of the dead-stop volume (15 µL) and final concentration factor, providing reliable and consistent sample processing. They can be used effectively at a maximum centrifugal force of 15,000 x g at room temperature. Centrifugal force, temperature and sample volume, concentration and viscosity affect filtration rate. Optimize centrifugal time for each application.
Related Products
Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 30K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 100K MWCO, 0.5 mL
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 10 minutes or less
• Excellent protein recovery—unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions
• Instrument-compatible—use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning
• Versatile—easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low non-specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. Sample concentration of 10- to 30-fold can be achieved within 10 minutes.
Applications:
• Protein concentration with tissue culture media, antiserum or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns
• Separating large molecules from small molecules
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.05 mg/mL.
Pierce Protein Concentrators PES allow accurate control of the dead-stop volume (15 µL) and final concentration factor, providing reliable and consistent sample processing. They can be used effectively at a maximum centrifugal force of 15,000 x g at room temperature. Centrifugal force, temperature and sample volume, concentration and viscosity affect filtration rate. Optimize centrifugal time for each application.
Related Products
Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 30K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 100K MWCO, 0.5 mL
Pierce™ Protein Concentrator PES, 3K MWCO, 2-6 mL
Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 3K and can be used for processing sample volumes ranging from 2 to 6 mL.
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.
Please see Cat. No. 88514 for more information about this product.
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.
Please see Cat. No. 88514 for more information about this product.
Pierce™ Protein Concentrator PES, 3K MWCO, 5-20 mL (Thermo Scientific™)
Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 3K and can be used for processing sample volumes ranging from 5 to 20 mL.
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.
Microcentrifuge Spin Columns (Invitrogen™)
The microcentrifuge spin columns included with the FastTrack® 2.0 and Micro-FastTrack™ 2.0 Kits are available separately. The columns are made from polypropylene and are designed to enhance the speed and efficiency of polyA+ RNA isolation. The columns are sterilized and RNase-free.
Centri-Sep™ 96-Well Plates (Applied Biosystems™)
Centri-Sep™ 96's hydrated matrix enables effective and reliable high-throughput removal of excess BigDye® terminators from DNA sequencing reactions, giving you clean sequencing reads with uniform signal intensity starting with the first base. The plate's 96-well format enables you to purify 96 samples simultaneously, either manually or by robotics, within 15 minutes.
• Processed faster than ultrafiltration columns
• Rapid and efficient separations
• Buffer is not preselected
• Convenient 20-100 µL sample size
• Dry gel format
• Room Temperature Storage
• Microcentrifuge format
Designed for Performance
The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Sep unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.
Excellent Recovery
The Centri-Sep™ gel will provide excellent recovery of DNA fragments with sizes greater than 16 base pairs or 25-mer, while removing 98% of salts, NTP's and other unwanted low molecular-weight impurities.
Versatile
The column gel is hydrated with either reagent-grade water or a suitable buffer or your choice, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the intersititial fluid. Your sample is then applied to the column and spun again, leaving you not only with a sample purified through removal of low-molecular-weight components, but also exchanged into the buffer of your choice.
For Research Use Only. Not for use in diagnostics procedures.
• Processed faster than ultrafiltration columns
• Rapid and efficient separations
• Buffer is not preselected
• Convenient 20-100 µL sample size
• Dry gel format
• Room Temperature Storage
• Microcentrifuge format
Designed for Performance
The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Sep unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.
Excellent Recovery
The Centri-Sep™ gel will provide excellent recovery of DNA fragments with sizes greater than 16 base pairs or 25-mer, while removing 98% of salts, NTP's and other unwanted low molecular-weight impurities.
Versatile
The column gel is hydrated with either reagent-grade water or a suitable buffer or your choice, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the intersititial fluid. Your sample is then applied to the column and spun again, leaving you not only with a sample purified through removal of low-molecular-weight components, but also exchanged into the buffer of your choice.
For Research Use Only. Not for use in diagnostics procedures.
Pierce™ Protein Concentrators PES, 30K MWCO, 0.5 mL (Thermo Scientific™)
Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting and buffer exchange of biological samples. This product has a molecular-weight cutoff of 30K, and can be used for processing sample volumes up to 0.5 mL.
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 10 minutes or less
• Excellent protein recovery—unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions
• Instrument-compatible—use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning
• Versatile—easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low non-specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. Sample concentration of 10- to 30-fold can be achieved within 10 minutes.
Applications:
• Protein concentration with tissue culture media, antiserum or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns
• Separating large molecules from small molecules
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.05 mg/mL.
Pierce Protein Concentrators PES allow accurate control of the dead-stop volume (15 µL) and final concentration factor, providing reliable and consistent sample processing. They can be used effectively at a maximum centrifugal force of 15,000 x g at room temperature. Centrifugal force, temperature and sample volume, concentration and viscosity affect filtration rate. Optimize centrifugal time for each application.
Related Products
Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 3K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 100K MWCO, 0.5 mL
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 10 minutes or less
• Excellent protein recovery—unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions
• Instrument-compatible—use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning
• Versatile—easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low non-specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. Sample concentration of 10- to 30-fold can be achieved within 10 minutes.
Applications:
• Protein concentration with tissue culture media, antiserum or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns
• Separating large molecules from small molecules
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.05 mg/mL.
Pierce Protein Concentrators PES allow accurate control of the dead-stop volume (15 µL) and final concentration factor, providing reliable and consistent sample processing. They can be used effectively at a maximum centrifugal force of 15,000 x g at room temperature. Centrifugal force, temperature and sample volume, concentration and viscosity affect filtration rate. Optimize centrifugal time for each application.
Related Products
Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 3K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 100K MWCO, 0.5 mL
Exosome Spin Columns (MW 3000) (Invitrogen™)
Exosome Spin Columns (MW 3000) enable fast removal of unincorporated dye from exosome labeling reactions. They also allow the user to perform buffer exchange on the exosomes or to remove any low molecular weight (MW ≤ 3000) admixtures from the exosome preparation such as salts, nucleotides, and short oligonucleotides.
• Fast, efficient removal of unincorporated dye from the labeled exosomes
• Buffer exchange, desalting, removal of low molecular weight contaminants
• Replaces more time-consuming purification methods (such as ultra-centrifugation)
To use, simply rehydrate the spin column, centrifuge to remove the interstitial fluid, add the exosome sample to the top of the column, and centrifuge again to elute the exosomes (low molecular weight contaminants will be retained by the column).
• Fast, efficient removal of unincorporated dye from the labeled exosomes
• Buffer exchange, desalting, removal of low molecular weight contaminants
• Replaces more time-consuming purification methods (such as ultra-centrifugation)
To use, simply rehydrate the spin column, centrifuge to remove the interstitial fluid, add the exosome sample to the top of the column, and centrifuge again to elute the exosomes (low molecular weight contaminants will be retained by the column).
Pierce™ Centrifuge Columns, 10 mL (Thermo Scientific™)
Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µL to 10 mL of an affinity support for purification.
Column details:
• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 22 mL (10 mL resin bed, 12 mL reservoir)
• Resin volume: 10 mL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard 50 mL conical centrifuge tubes
• Caps: Screw-top cap
• Twist-off bottom and press-on cap to reseal
Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.
Applications:
• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange
Related Products
Pierce™ Centrifuge Columns, 0.8 mL
Pierce™ Centrifuge Columns, 2 mL
Pierce™ Centrifuge Columns, 5 mL
Column Extenders for Pierce™ Centrifuge Columns
Column details:
• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 22 mL (10 mL resin bed, 12 mL reservoir)
• Resin volume: 10 mL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard 50 mL conical centrifuge tubes
• Caps: Screw-top cap
• Twist-off bottom and press-on cap to reseal
Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.
Applications:
• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange
Related Products
Pierce™ Centrifuge Columns, 0.8 mL
Pierce™ Centrifuge Columns, 2 mL
Pierce™ Centrifuge Columns, 5 mL
Column Extenders for Pierce™ Centrifuge Columns
Pierce™ Protein Concentrator PES, 30K MWCO, 5-20 mL
Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 30K and can be used for processing sample volumes ranging from 5 to 20 mL.
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.
Please see Cat. No. 88529 for more information about this product.
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.
Please see Cat. No. 88529 for more information about this product.
PureLink™ 96 Receiver Plates (Invitrogen™)
PureLink® 96: Sample size 1.3 ml culture; Typical yields up to 3-5 µg
PureLink® 384: Sample size 200 µl culture; Typical yields up to 2-3 µg
––Purify 96 or 384 plasmid DNA samples simultaneously
–centrifugation method eliminates cross-contamination
The PureLink® 96 Plasmid Purification System is designed for high-throughput isolation of high-copy number plasmids. Following the PureLink® 96 Plasmid Purification Systems protocol, up to 96 plasmid DNA samples can be simultaneously purified. The novel technology eliminates time-consuming cell harvesting and alkaline lysis steps, saving time and effort. The unique centrifugationbased format of the PureLink® 96 Plasmid Purification System eliminates opportunities for cross-contamination between wells, a critical factor in PCR and microarrays. The isolated plasmid DNA is ideal for use in automated sequencing reactions, offering superior read lengths and data quality. Samples may be processed 96 at a time or in smaller increments. The PureLink® 96 Plasmid Purification System is compatible with a variety of centrifuges, robots, pipetting devices, automated sequencers, and sequencing chemistries.
The PureLink® 384 Plasmid Purification Plate is designed for ultra highthroughput DNA isolation. By utilizing the same technology as the PureLink® 96 Plasmid Purification System, the PureLink® 384 Plasmid Purification Plate reduces time and effort while reliably isolating high-quality plasmid DNA for DNA sequencing, PCR, and restriction digestion.
PureLink® 384: Sample size 200 µl culture; Typical yields up to 2-3 µg
––Purify 96 or 384 plasmid DNA samples simultaneously
–centrifugation method eliminates cross-contamination
The PureLink® 96 Plasmid Purification System is designed for high-throughput isolation of high-copy number plasmids. Following the PureLink® 96 Plasmid Purification Systems protocol, up to 96 plasmid DNA samples can be simultaneously purified. The novel technology eliminates time-consuming cell harvesting and alkaline lysis steps, saving time and effort. The unique centrifugationbased format of the PureLink® 96 Plasmid Purification System eliminates opportunities for cross-contamination between wells, a critical factor in PCR and microarrays. The isolated plasmid DNA is ideal for use in automated sequencing reactions, offering superior read lengths and data quality. Samples may be processed 96 at a time or in smaller increments. The PureLink® 96 Plasmid Purification System is compatible with a variety of centrifuges, robots, pipetting devices, automated sequencers, and sequencing chemistries.
The PureLink® 384 Plasmid Purification Plate is designed for ultra highthroughput DNA isolation. By utilizing the same technology as the PureLink® 96 Plasmid Purification System, the PureLink® 384 Plasmid Purification Plate reduces time and effort while reliably isolating high-quality plasmid DNA for DNA sequencing, PCR, and restriction digestion.
Chargeswitch™ Pro Filter Collection Tube (Invitrogen™)
The Chargeswitch® -Pro Filter Collection Tube is a 50 ml conical tube that will accommodate the centrifuge protocol of Chargeswitch® Pro Filter Plasmid Midi and Maxi kits
