Shop All CHO & DG44 Media
CD FortiCHO™ Medium (Gibco™)
Le milieu de culture CD FortiCHO™ est spécifiquement conçu pour offrir la meilleure des performances de culture par lot et par lot nourricier et le meilleur rendement avec des cellules CHO recombinantes dans un environnement chimiquement défini. La formulation exempte de protéines et exempte de composants d’origine animale garantit la constance et contribue à réduire le besoin en analyse d’agents fortuits. Avec le milieu CD FortiCHO™, les cultures cellulaires durent plus de 2 jours de plus que les alternatives de la concurrence, ce qui signifie une baisse de la concentration des HCP (protéines hôtes) pour l’élimination dans la purification en aval.
Le milieu de culture CD FortiCHO™ est fortifié pour prendre en charge une croissance des cellules CHO recombinantes et une expression des protéines maximales dans les cultures par lot en suspension, offrant 120 % de plus de croissance et de production que certains milieux de la concurrence avec une simple supplémentation en glucose. Le milieu de culture CD FortiCHO™ peut fournir jusqu’à 75 % de croissance et de production de lots nourriciers en plus par rapport à la culture de lots nourriciers au glucose uniquement pour certaines lignées cellulaires et applications lorsqu’il est utilisé avec le supplément nutriant CD EfficientFeed™.
Quand utiliser le milieu CD FortiCHO™
• La lignée cellulaire utilisée est une lignée cellulaire CHO K1, GS CHO ou CHO-S™ transfectée.
• Des densités cellulaires maximum de culture de lots et des titres protéiniques sont nécessaires.
• Pour optimiser les titres, vous disposez de suffisamment de temps pour adapter les cellules à un nouveau milieu.
• La santé cellulaire au moment de la récolte est l’une des priorités essentielles pour le traitement en aval
Quand envisager le recours à un autre milieu de culture Gibco®
Envisagez le recours à un milieu de culture CD OptiCHO™ dans les cas suivants :
• Vous utilisez une lignée cellulaire CHO transfectée autre que CHO K1, GS CHO ou CHO-S™
• Vous intégrerez un processus d’optimisation des stratégies nourricières à vos travaux de sélection d’un milieu de culture de base en vue d’optimiser la productivité
• Votre lignée cellulaire CHO est “délicate”, c’est-à-dire qu’elle a des difficultés à se développer ou à s’adapter
Envisagez le recours à un milieu de culture CD CHO dans les cas suivants :
• Un milieu de culture CD CHO est recommandé pour vos cellules GS CHO
• Vos cellules ont été cultivées correctement dans un milieu CD CHO dans le passé et votre processus doit bientôt faire l’objet d’un archivage réglementaire (DMF disponible)
Envisagez le recours à CD DG44 dans les cas suivants :
• Vous utilisez des cellules ovariennes de hamster (CHO) DG44 ou DXB11 parentales (dhfr-)
Formulations de milieu sec
Pour un accès anticipé au milieu CD FortiCHO™ AGT™, veuillez contacter les services techniques de Gibco®.
Créez un milieu sur mesure adapté à vos besoins
Nous proposons des formats spécialisés, des conditionnements et des niveaux de qualité adaptés à vos besoins, qu’il s’agisse d’une expérience sur paillasse ou d’un processus de bioproduction à grande échelle. Les services personnalisés de milieux Gibco® conformes aux BPFa vous permettent de tirer profit de toutes les capacités de production et des nombreuses années d’expérience de nos spécialistes en matière de recherche et développement et d’applications de bioproduction.
Le milieu de culture CD FortiCHO™ est fortifié pour prendre en charge une croissance des cellules CHO recombinantes et une expression des protéines maximales dans les cultures par lot en suspension, offrant 120 % de plus de croissance et de production que certains milieux de la concurrence avec une simple supplémentation en glucose. Le milieu de culture CD FortiCHO™ peut fournir jusqu’à 75 % de croissance et de production de lots nourriciers en plus par rapport à la culture de lots nourriciers au glucose uniquement pour certaines lignées cellulaires et applications lorsqu’il est utilisé avec le supplément nutriant CD EfficientFeed™.
Quand utiliser le milieu CD FortiCHO™
• La lignée cellulaire utilisée est une lignée cellulaire CHO K1, GS CHO ou CHO-S™ transfectée.
• Des densités cellulaires maximum de culture de lots et des titres protéiniques sont nécessaires.
• Pour optimiser les titres, vous disposez de suffisamment de temps pour adapter les cellules à un nouveau milieu.
• La santé cellulaire au moment de la récolte est l’une des priorités essentielles pour le traitement en aval
Quand envisager le recours à un autre milieu de culture Gibco®
Envisagez le recours à un milieu de culture CD OptiCHO™ dans les cas suivants :
• Vous utilisez une lignée cellulaire CHO transfectée autre que CHO K1, GS CHO ou CHO-S™
• Vous intégrerez un processus d’optimisation des stratégies nourricières à vos travaux de sélection d’un milieu de culture de base en vue d’optimiser la productivité
• Votre lignée cellulaire CHO est “délicate”, c’est-à-dire qu’elle a des difficultés à se développer ou à s’adapter
Envisagez le recours à un milieu de culture CD CHO dans les cas suivants :
• Un milieu de culture CD CHO est recommandé pour vos cellules GS CHO
• Vos cellules ont été cultivées correctement dans un milieu CD CHO dans le passé et votre processus doit bientôt faire l’objet d’un archivage réglementaire (DMF disponible)
Envisagez le recours à CD DG44 dans les cas suivants :
• Vous utilisez des cellules ovariennes de hamster (CHO) DG44 ou DXB11 parentales (dhfr-)
Formulations de milieu sec
Pour un accès anticipé au milieu CD FortiCHO™ AGT™, veuillez contacter les services techniques de Gibco®.
Créez un milieu sur mesure adapté à vos besoins
Nous proposons des formats spécialisés, des conditionnements et des niveaux de qualité adaptés à vos besoins, qu’il s’agisse d’une expérience sur paillasse ou d’un processus de bioproduction à grande échelle. Les services personnalisés de milieux Gibco® conformes aux BPFa vous permettent de tirer profit de toutes les capacités de production et des nombreuses années d’expérience de nos spécialistes en matière de recherche et développement et d’applications de bioproduction.
CD OptiCHO™ AGT™ Medium (Gibco™)
CD OptiCHO™ medium is specifically designed to offer high performance and yield with recombinant CHO cells in a chemically defined fed-batch environment. The protein-free, animal origin free formulation is designed to provide consistency and reduces the need to screen for adventitious agents. CD OptiCHO™ medium builds on Gibco® CD CHO’s legacy of consistent cell growth and titers, while offering up to 40% better titer and cell growth over select competitors. Thanks to its low osmolality and advanced CHO CD EfficientFeed™ Liquid Nutrient Supplement Kit feeding options, CD OptiCHO™ medium is capable of gaining 2-5 fold increases in fed-batch productivity over batch culture.
When to Use CD OptiCHO™ Medium
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
When to Use CD OptiCHO™ Medium
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
ExpiCHO™ Expression Medium (Gibco™)
ExpiCHO™ Expression Medium is a GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. It is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133) for scalable transient protein production.
ExpiCHO Expression Medium:
• Supports growth of suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability
• Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies of >90% with ExpiFectamine™ CHO transfection reagent
• Supports transient transfection of suspension CHO cells at a density of 6 x 106 cells per mL
• Facilitates sustained, high-level expression of high-density transiently transfected cultures, achieving yields of up to 3 g per L of recombinant protein
• Supports growth and transfection of CHO cells in culture formats from <1 mL in multi-well plates to >10 L in benchtop bioreactors
• Does not contain phenol red
ExpiCHO Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically-defined formulation results in high reproducibility and lot-to-lot reliability. For highest level transient protein expression, the medium is used in combination with a matched culture feed, ExpiCHO™ Feed, which is supplied as part of the ExpiFectamine™ CHO Transfection Kit (Cat. No. A29129). ExpiCHO Expression Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL in multi-well plates to >10 L in bioreactors.
ExpiCHO Expression Medium:
• Supports growth of suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability
• Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies of >90% with ExpiFectamine™ CHO transfection reagent
• Supports transient transfection of suspension CHO cells at a density of 6 x 106 cells per mL
• Facilitates sustained, high-level expression of high-density transiently transfected cultures, achieving yields of up to 3 g per L of recombinant protein
• Supports growth and transfection of CHO cells in culture formats from <1 mL in multi-well plates to >10 L in benchtop bioreactors
• Does not contain phenol red
ExpiCHO Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically-defined formulation results in high reproducibility and lot-to-lot reliability. For highest level transient protein expression, the medium is used in combination with a matched culture feed, ExpiCHO™ Feed, which is supplied as part of the ExpiFectamine™ CHO Transfection Kit (Cat. No. A29129). ExpiCHO Expression Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL in multi-well plates to >10 L in bioreactors.
CHO-S-SFM II, with hypoxanthine and thymidine (Gibco™)
CHO-S-SFM II, is a complete, serum-free, low-protein (less than 100 µ/ml) medium optimized for the growth and maintenance of Chinese hamster ovary (CHO) cells in suspension culture for production of recombinant proteins. It is supplemented with hypoxanthine and thymidine (the non-supplemented equivalent is also available). This ready-to-use medium is suitable for the suspension culturing of CHO and other cells in batch, continuous, and perfusion culture systems. A CHO-S-SFM II medium Drug Master File (DMF) has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative.
This medium contains components of animal origin. If growth factors or hydrolysates, found in competitor media, are to be avoided, consider CD CHO, CD OptiCHO™ or CD FortiCHO™ media.
When to use CHO-S-SFM II Medium
• Your cells have been successfully cultured in CHO-S-SFM II medium in the past and your process is nearing a regulatory filing (DMF available)
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
This medium contains components of animal origin. If growth factors or hydrolysates, found in competitor media, are to be avoided, consider CD CHO, CD OptiCHO™ or CD FortiCHO™ media.
When to use CHO-S-SFM II Medium
• Your cells have been successfully cultured in CHO-S-SFM II medium in the past and your process is nearing a regulatory filing (DMF available)
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
Dynamis™ AGT™ Medium (Gibco™)
Gibco® Dynamis™ AGT™ dry format medium is specifically designed to offer the highest batch and fed-batch culture performance and yield with recombinant CHO cells in a chemically defined environment. The chemically defined, protein-free, animal origin component-free formulation provides the power to achieve high titers, start process development faster, and streamline or simplify transfer to manufacturing scale.
Power to achieve higher titers
Dynamis™ AGT™ Medium is fortified to help maximize recombinant CHO cell growth and protein expression in suspension batch culture, offering 170% greater production than select competitive media with simple glucose supplementation by day 14. Cultures grown in Dynamis™ AGT™ Medium extend to even higher titers demonstrating good cell viability out to day 21. This medium can also provide up to 60% greater fed-batch growth and production over glucose-only fed-batch culture for selected cell lines and applications when used with EfficientFeed™ C+ AGT™ Supplement.
Power to start process development faster
As a fortified medium, Dynamis™ AGT™ provides the power to get to a high cell density over 10 million cells per mL faster than competitive products and sustains that cell density longer by just maintaining glucose levels. The viable cell growth achieved make it a good choice for scale-up if you have started with CD FortiCHO™ medium. When paired with EfficientFeed™ C+ AGT™ Supplement, it provides a fast and efficient way to develop a process.
The power to streamline transfer to manufacturing
By choosing Dynamis™ AGT™ Medium, you obtain not only the benefits of the medium formulation, but also the benefits of AGT™ dry format technology. This all-in-one product is designed to simplify scale-up and manufacturing by being easy to reconstitute (just add water), with no adjustments of pH or osmolarity necessary. This can help provide efficiencies through supply chain simplicity, reconstitution simplicity, and therefore help reduce expense.
Use Dynamis™ AGT™ Medium when:
• The cell line you are using is a transfected CHO K1, GS CHO, or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
When to consider another Gibco® medium
Consider CD OptiCHO™ Medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO, or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is hard to grow or adapt
Consider CD CHO Medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 Medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Design your own media specific to your needs
Whether it’s a bench-level experiment or large-scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services, you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
Gibco® is a registered trademark of ThermoFisher Scientific Inc. Dynamis™ and CD FortiCHO™ are trademarks of Thermo Fisher Scientific Inc., ActiCHO(TM) is a trademark of GE Healthcare Biosciences AB, BalanCD(R) is a registered trademark of Irvine Scientific Sales Co., Cellvento(R) is a registered trademark of Merck KGAA, EXCELL(R) is a registered trademark of SAFC, HyCell(R) is a registered trademark of GE Healthcare Biosciences AB, and PowerCHO(TM) is a registered trademark of Lonza.
Power to achieve higher titers
Dynamis™ AGT™ Medium is fortified to help maximize recombinant CHO cell growth and protein expression in suspension batch culture, offering 170% greater production than select competitive media with simple glucose supplementation by day 14. Cultures grown in Dynamis™ AGT™ Medium extend to even higher titers demonstrating good cell viability out to day 21. This medium can also provide up to 60% greater fed-batch growth and production over glucose-only fed-batch culture for selected cell lines and applications when used with EfficientFeed™ C+ AGT™ Supplement.
Power to start process development faster
As a fortified medium, Dynamis™ AGT™ provides the power to get to a high cell density over 10 million cells per mL faster than competitive products and sustains that cell density longer by just maintaining glucose levels. The viable cell growth achieved make it a good choice for scale-up if you have started with CD FortiCHO™ medium. When paired with EfficientFeed™ C+ AGT™ Supplement, it provides a fast and efficient way to develop a process.
The power to streamline transfer to manufacturing
By choosing Dynamis™ AGT™ Medium, you obtain not only the benefits of the medium formulation, but also the benefits of AGT™ dry format technology. This all-in-one product is designed to simplify scale-up and manufacturing by being easy to reconstitute (just add water), with no adjustments of pH or osmolarity necessary. This can help provide efficiencies through supply chain simplicity, reconstitution simplicity, and therefore help reduce expense.
Use Dynamis™ AGT™ Medium when:
• The cell line you are using is a transfected CHO K1, GS CHO, or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
When to consider another Gibco® medium
Consider CD OptiCHO™ Medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO, or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is hard to grow or adapt
Consider CD CHO Medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 Medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Design your own media specific to your needs
Whether it’s a bench-level experiment or large-scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services, you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
Gibco® is a registered trademark of ThermoFisher Scientific Inc. Dynamis™ and CD FortiCHO™ are trademarks of Thermo Fisher Scientific Inc., ActiCHO(TM) is a trademark of GE Healthcare Biosciences AB, BalanCD(R) is a registered trademark of Irvine Scientific Sales Co., Cellvento(R) is a registered trademark of Merck KGAA, EXCELL(R) is a registered trademark of SAFC, HyCell(R) is a registered trademark of GE Healthcare Biosciences AB, and PowerCHO(TM) is a registered trademark of Lonza.
FreeStyle™ CHO Expression Medium (Gibco™)
FreeStyle™ CHO Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation.
This medium requires supplementation with 8 mM glutamine before use. Gibco® FreeStyle™ CHO Expression Medium features:
• Formulation without hypoxanthine and thymidine for use with or without dhfr systems
• Ability to support high-density suspension growth and transient transfection of CHO-S cells
• Animal origin–free, protein-free and chemically defined formulation
• Easy use as part of the FreeStyle™ MAX CHO Expression System for protein production
• Scalability in spinner flasks and bioreactors
Formulation without hypoxanthine and thymidine for use with or without dhfr systems
Gibco® FreeStyle™ CHO Expression Medium is made without hypoxanthine and thymidine for use in dihydrofolate reductase (dhfr) amplification systems. Non-dhfr systems require the addition of HT Supplement before use.
Ability to support high-density suspension growth and transient transfection of CHO-S cells
Gibco® FreeStyle™ CHO cells in suspension can be cultured directly into Gibco® FreeStyle™ CHO Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ CHO Expression Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations. Cells cultured in Gibco® FreeStyle™ CHO Expression Medium can be transfected using lipid-based transfection reagents, such as Freestyle™ MAX reagent.
Animal origin–free, protein-free and chemically defined formulation
Gibco® FreeStyle™ CHO Expression Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.
Easy use as part of the FreeStyle™ MAX CHO Expression System for protein production
The FreeStyle™ MAX CHO Expression System is a breakthrough technology for rapid (see figure) and high-yield mammalian protein production in 30 mL and 1 L batch sizes (see figure). The FreeStyle™ MAX CHO Expression System combines GIBCO® FreeStyle™ media, FreeStyle™ MAX reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293-F cells in one kit.
Scalability in spinner flasks and bioreactors
Protein production using Gibco® FreeStyle™ CHO Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ CHO Expression Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.
cGMP manufacturing and quality system
Gibco® FreeStyle™ CHO Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
This medium requires supplementation with 8 mM glutamine before use. Gibco® FreeStyle™ CHO Expression Medium features:
• Formulation without hypoxanthine and thymidine for use with or without dhfr systems
• Ability to support high-density suspension growth and transient transfection of CHO-S cells
• Animal origin–free, protein-free and chemically defined formulation
• Easy use as part of the FreeStyle™ MAX CHO Expression System for protein production
• Scalability in spinner flasks and bioreactors
Formulation without hypoxanthine and thymidine for use with or without dhfr systems
Gibco® FreeStyle™ CHO Expression Medium is made without hypoxanthine and thymidine for use in dihydrofolate reductase (dhfr) amplification systems. Non-dhfr systems require the addition of HT Supplement before use.
Ability to support high-density suspension growth and transient transfection of CHO-S cells
Gibco® FreeStyle™ CHO cells in suspension can be cultured directly into Gibco® FreeStyle™ CHO Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ CHO Expression Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations. Cells cultured in Gibco® FreeStyle™ CHO Expression Medium can be transfected using lipid-based transfection reagents, such as Freestyle™ MAX reagent.
Animal origin–free, protein-free and chemically defined formulation
Gibco® FreeStyle™ CHO Expression Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.
Easy use as part of the FreeStyle™ MAX CHO Expression System for protein production
The FreeStyle™ MAX CHO Expression System is a breakthrough technology for rapid (see figure) and high-yield mammalian protein production in 30 mL and 1 L batch sizes (see figure). The FreeStyle™ MAX CHO Expression System combines GIBCO® FreeStyle™ media, FreeStyle™ MAX reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293-F cells in one kit.
Scalability in spinner flasks and bioreactors
Protein production using Gibco® FreeStyle™ CHO Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ CHO Expression Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.
cGMP manufacturing and quality system
Gibco® FreeStyle™ CHO Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
CD CHO AGT™ Medium (Gibco™)
CD CHO AGT™ (Advanced Granulation Technology) medium is a protein-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. The AGT™ dry media format is designed to provide increased consistency and productivity across all stages of production from development to commercial manufacturing. CD CHO AGT™ medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates.
When to Use CD CHO Medium
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
When to Use CD CHO Medium
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
1X CD OptiCHO™ Medium (Gibco™)
CD OptiCHO™ medium is specifically designed to offer high performance and yield with recombinant CHO cells in a chemically defined fed-batch environment. The protein-free, animal origin free formulation is designed to provide consistency and reduces the need to screen for adventitious agents. CD OptiCHO™ medium builds on Gibco® CD CHO’s legacy of consistent cell growth and titers, while offering up to 40% better titer and cell growth over select competitors. Thanks to its low osmolality and advanced CHO CD EfficientFeed™ Liquid Nutrient Supplement Kit feeding options, CD OptiCHO™ medium is capable of gaining 2-5 fold increases in fed-batch productivity over batch culture.
When to Use CD OptiCHO™ Medium
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Dry Media Formulations
Gibco® liquid media are available in an AGT™ (Advanced Granulation Technology) dry media format that is designed to provide increased consistency and productivity across all stages of production from development to commercial manufacturing. AGT™ media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their liquid media counterparts.
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
When to Use CD OptiCHO™ Medium
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Dry Media Formulations
Gibco® liquid media are available in an AGT™ (Advanced Granulation Technology) dry media format that is designed to provide increased consistency and productivity across all stages of production from development to commercial manufacturing. AGT™ media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their liquid media counterparts.
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
CHO-S-SFM II (without hypoxanthine or thymidine) (Gibco™)
CHO-S-SFM II, is a complete, serum-free, low-protein (less than 100 µg/ml) medium optimized for the growth and maintenance of Chinese hamster ovary (CHO) cells in suspension culture for production of recombinant proteins. This medium contains no thymidine or hypoxanthine (the supplemented medium is also available). This ready-to-use medium is suitable for the suspension culturing of CHO and other cells in batch, continuous, and perfusion culture systems. A CHO-S-SFM II medium Drug Master File (DMF) has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative.
This medium contains components of animal origin. If growth factors or hydrolysates, found in competitor media, are to be avoided, consider CD CHO, CD OptiCHO™ or CD FortiCHO™ media.
When to use CHO-S-SFM II Medium
• Your cells have been successfully cultured in CHO-S-SFM II medium in the past and your process is nearing a regulatory filing (DMF available)
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
This medium contains components of animal origin. If growth factors or hydrolysates, found in competitor media, are to be avoided, consider CD CHO, CD OptiCHO™ or CD FortiCHO™ media.
When to use CHO-S-SFM II Medium
• Your cells have been successfully cultured in CHO-S-SFM II medium in the past and your process is nearing a regulatory filing (DMF available)
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
Dynamis™ Medium (Gibco™)
Gibco® Dynamis™ liquid medium is specifically designed to offer the highest batch and fed-batch culture performance and yield with recombinant CHO cells in a chemically defined environment. The chemically defined, protein-free, animal origin component-free formulation provides the power to achieve high titers, start process development faster and streamline or simplify transfer to manufacturing scale.
Power to achieve higher titers
Dynamis™ AGT™ Medium is fortified to help maximize recombinant CHO cell growth and protein expression in suspension batch culture, offering 170% greater production than select competitive media with simple glucose supplementation by day 14. Cultures grown in Dynamis™ AGT™ Medium extend to even higher titers demonstrating good cell viability out to day 21. This medium can also provide up to 60% greater fed-batch growth and production over glucose-only fed-batch culture for selected cell lines and applications when used with EfficientFeed™ C+ AGT™ Supplement.
Power to start process development faster
As a fortified medium, Dynamis™ AGT™ provides the power to get to a high cell density over 10 million cells per mL faster than competitive products and sustains that cell density longer by just maintaining glucose levels. The viable cell growth achieved make it a good choice for scale-up if you have started with CD FortiCHO™ medium. When paired with EfficientFeed™ C+ AGT™ Supplement, it provides a fast and efficient way to develop a process.
The power to streamline transfer to manufacturing
Dynamis™ liquid medium is made by reconstitution of Dynamis™ AGT™ dry format into the liquid format. Therefore, you simplify scale-up and manufacturing in AGT™ dry format because you know the liquid will behave exactly like the AGT™ format. The AGT™ dry format scale-up provides easy to reconstitute (just add water) use including auto-pH and auto-osmolarity. This can help provide efficiencies in supply chain complexity, reconstitution complexity and therefore overall expense.
When to Use Dynamis™ Medium
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
When to Consider Another Gibco® Medium
Consider CD OptiCHO™ Medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
Consider CD CHO Medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 Medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
Gibco® is a registered trademark of ThermoFisher Scientific Inc. Dynamis™ and CD FortiCHO™ are trademarks of Thermo Fisher Scientific Inc., ActiCHO(TM) is a trademark of GE Healthcare Biosciences AB, BalanCD(R) is a registered trademark of Irvine Scientific Sales Co., Cellvento(R) is a registered trademark of Merck KGAA, EXCELL(R) is a registered trademark of SAFC, HyCell(R) is a registered trademark of GE Healthcare Biosciences AB, and PowerCHO(TM) is a registered trademark of Lonza.
Power to achieve higher titers
Dynamis™ AGT™ Medium is fortified to help maximize recombinant CHO cell growth and protein expression in suspension batch culture, offering 170% greater production than select competitive media with simple glucose supplementation by day 14. Cultures grown in Dynamis™ AGT™ Medium extend to even higher titers demonstrating good cell viability out to day 21. This medium can also provide up to 60% greater fed-batch growth and production over glucose-only fed-batch culture for selected cell lines and applications when used with EfficientFeed™ C+ AGT™ Supplement.
Power to start process development faster
As a fortified medium, Dynamis™ AGT™ provides the power to get to a high cell density over 10 million cells per mL faster than competitive products and sustains that cell density longer by just maintaining glucose levels. The viable cell growth achieved make it a good choice for scale-up if you have started with CD FortiCHO™ medium. When paired with EfficientFeed™ C+ AGT™ Supplement, it provides a fast and efficient way to develop a process.
The power to streamline transfer to manufacturing
Dynamis™ liquid medium is made by reconstitution of Dynamis™ AGT™ dry format into the liquid format. Therefore, you simplify scale-up and manufacturing in AGT™ dry format because you know the liquid will behave exactly like the AGT™ format. The AGT™ dry format scale-up provides easy to reconstitute (just add water) use including auto-pH and auto-osmolarity. This can help provide efficiencies in supply chain complexity, reconstitution complexity and therefore overall expense.
When to Use Dynamis™ Medium
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
When to Consider Another Gibco® Medium
Consider CD OptiCHO™ Medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
Consider CD CHO Medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 Medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
Gibco® is a registered trademark of ThermoFisher Scientific Inc. Dynamis™ and CD FortiCHO™ are trademarks of Thermo Fisher Scientific Inc., ActiCHO(TM) is a trademark of GE Healthcare Biosciences AB, BalanCD(R) is a registered trademark of Irvine Scientific Sales Co., Cellvento(R) is a registered trademark of Merck KGAA, EXCELL(R) is a registered trademark of SAFC, HyCell(R) is a registered trademark of GE Healthcare Biosciences AB, and PowerCHO(TM) is a registered trademark of Lonza.
CD OptiCHO™ AGT™ Medium (Gibco™)
CD OptiCHO™ medium is specifically designed to offer high performance and yield with recombinant CHO cells in a chemically defined fed-batch environment. The protein-free, animal origin free formulation is designed to provide consistency and reduces the need to screen for adventitious agents. CD OptiCHO™ medium builds on Gibco® CD CHO’s legacy of consistent cell growth and titers, while offering up to 40% better titer and cell growth over select competitors. Thanks to its low osmolality and advanced CHO CD EfficientFeed™ Liquid Nutrient Supplement Kit feeding options, CD OptiCHO™ medium is capable of gaining 2-5 fold increases in fed-batch productivity over batch culture.
When to Use CD OptiCHO™ Medium
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
When to Use CD OptiCHO™ Medium
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
CD CHO Medium (Gibco™)
Le milieu CD CHO, sans protéines et sans sérum, a été défini chimiquement et optimisé pour la croissance des cellules ovariennes de hamster (CHO) et pour l’expression de protéines recombinantes dans des cultures en suspension. Doté de performances de qualité démontrées pendant plus de 10 ans, le milieu CD CHO ne contient pas de protéines ou composants peptidiques d’origine animal, végétal ou synthétique, ni de lysats ou hydrolysats non définis.
Quand utiliser le milieu CD CHO
• Un milieu de culture CD CHO est recommandé pour vos cellules GS CHO
• Vos cellules ont été cultivées correctement dans un milieu CD CHO dans le passé et votre processus doit bientôt faire l’objet d’un archivage réglementaire (DMF disponible)
Quand envisager le recours à un autre milieu de culture Gibco®
Envisagez le recours à un milieu de culture CD FortiCHO™ dans les cas suivants :
• La lignée cellulaire utilisée est une lignée cellulaire CHO K1, GS CHO ou CHO-S™ transfectée.
• Des densités cellulaires maximum de culture de lots et des titres protéiniques sont nécessaires.
• Pour optimiser les titres, vous disposez de suffisamment de temps pour adapter les cellules à un nouveau milieu.
• La santé cellulaire au moment de la récolte est l’une des priorités essentielles pour le traitement en aval
Envisagez le recours à un milieu de culture CD OptiCHO™ dans les cas suivants :
• Vous utilisez une lignée cellulaire CHO transfectée autre que CHO K1, GS CHO ou CHO-S™
• Vous intégrerez un processus d’optimisation des stratégies nourricières à vos travaux de sélection d’un milieu de culture de base en vue d’optimiser la productivité
• Votre lignée cellulaire CHO est “délicate”, c’est-à-dire qu’elle a des difficultés à se développer ou à s’adapter
Envisagez le recours à un milieu de culture CD DG44 dans les cas suivants :
• Vous utilisez des cellules ovariennes de hamster (CHO) DG44 ou DXB11 parentales (dhfr-)
Formulations de milieu sec
Les milieux liquides Gibco® sont disponibles dans un format de milieu sec AGT™ (Advanced Granulation Technology) qui offre une plus grande productivité et une meilleure constance à toutes les étapes de production, du développement à la production commerciale. Les milieux AGT™ ne contiennent pas de composants d’origine animale et partagent les mêmes performances et la même formulation définie chimiquement, sans sérum ni protéines, que leurs équivalents liquides.
Créez un milieu sur mesure adapté à vos besoins
Nous proposons des formats spécialisés, des conditionnements et des niveaux de qualité adaptés à vos besoins, qu’il s’agisse d’une expérience sur paillasse ou d’un processus de bioproduction à grande échelle. Les services personnalisés de milieux Gibco® conformes aux BPFa vous permettent de tirer profit de toutes les capacités de production et des nombreuses années d’expérience de nos spécialistes en matière de recherche et développement et d’applications de bioproduction.
Quand utiliser le milieu CD CHO
• Un milieu de culture CD CHO est recommandé pour vos cellules GS CHO
• Vos cellules ont été cultivées correctement dans un milieu CD CHO dans le passé et votre processus doit bientôt faire l’objet d’un archivage réglementaire (DMF disponible)
Quand envisager le recours à un autre milieu de culture Gibco®
Envisagez le recours à un milieu de culture CD FortiCHO™ dans les cas suivants :
• La lignée cellulaire utilisée est une lignée cellulaire CHO K1, GS CHO ou CHO-S™ transfectée.
• Des densités cellulaires maximum de culture de lots et des titres protéiniques sont nécessaires.
• Pour optimiser les titres, vous disposez de suffisamment de temps pour adapter les cellules à un nouveau milieu.
• La santé cellulaire au moment de la récolte est l’une des priorités essentielles pour le traitement en aval
Envisagez le recours à un milieu de culture CD OptiCHO™ dans les cas suivants :
• Vous utilisez une lignée cellulaire CHO transfectée autre que CHO K1, GS CHO ou CHO-S™
• Vous intégrerez un processus d’optimisation des stratégies nourricières à vos travaux de sélection d’un milieu de culture de base en vue d’optimiser la productivité
• Votre lignée cellulaire CHO est “délicate”, c’est-à-dire qu’elle a des difficultés à se développer ou à s’adapter
Envisagez le recours à un milieu de culture CD DG44 dans les cas suivants :
• Vous utilisez des cellules ovariennes de hamster (CHO) DG44 ou DXB11 parentales (dhfr-)
Formulations de milieu sec
Les milieux liquides Gibco® sont disponibles dans un format de milieu sec AGT™ (Advanced Granulation Technology) qui offre une plus grande productivité et une meilleure constance à toutes les étapes de production, du développement à la production commerciale. Les milieux AGT™ ne contiennent pas de composants d’origine animale et partagent les mêmes performances et la même formulation définie chimiquement, sans sérum ni protéines, que leurs équivalents liquides.
Créez un milieu sur mesure adapté à vos besoins
Nous proposons des formats spécialisés, des conditionnements et des niveaux de qualité adaptés à vos besoins, qu’il s’agisse d’une expérience sur paillasse ou d’un processus de bioproduction à grande échelle. Les services personnalisés de milieux Gibco® conformes aux BPFa vous permettent de tirer profit de toutes les capacités de production et des nombreuses années d’expérience de nos spécialistes en matière de recherche et développement et d’applications de bioproduction.
CD DG44 Medium (Gibco™)
CD DG44 Medium is a chemically defined, protein-free medium optimized for the growth of dihydrofolate reductase deficient (DHFR-) Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture. CD DG44 fully chemically defined formulation contains no lysates, hydrolysates, nor proteins or peptide components of animal, plant, or synthetic origin.
When to Use CD DG44 Medium
• You use parental DG44 or DXB11 CHO cells (dhfr-)
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
When to Use CD DG44 Medium
• You use parental DG44 or DXB11 CHO cells (dhfr-)
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
CD CHO AGT™ Medium (Gibco™)
CD CHO AGT™ (Advanced Granulation Technology) medium is a protein-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. The AGT™ dry media format is designed to provide increased consistency and productivity across all stages of production from development to commercial manufacturing. CD CHO AGT™ medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates.
When to Use CD CHO Medium
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
When to Use CD CHO Medium
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
When to Consider Another Gibco® Medium
Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing
Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt
Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)
Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
