Shop All Neuronal Cell Media
Neurobasal™-A Medium, no D-glucose, no sodium pyruvate (Gibco™)
This is complete Neurobasal®-A medium without D-glucose and sodium pyruvate, and it is used to support growth of post-natal CNS neurons in applications where the presence of D-glucose and sodium pyruvate can cause interference.
For neural studies that require the absence of insulin, glucose, and sodium pyruvate, such as glucose response or insulin response studies, we recommend B-27® Supplement Minus Insulin as a supplement to this medium.
For other applications, we provide alternative formulations of B-27® Supplement.
For neural studies that require the absence of insulin, glucose, and sodium pyruvate, such as glucose response or insulin response studies, we recommend B-27® Supplement Minus Insulin as a supplement to this medium.
For other applications, we provide alternative formulations of B-27® Supplement.
Neurobasal™ Medium (Gibco™)
Le milieu de culture Neurobasal® est un milieu basal qui satisfait les exigences particulières de la culture de cellules neuronales prénatales et embryonnaires quand il est utilisé avec le supplément GIBCO® B-27®. Le milieu de culture Neurobasal® peut être utilisé pour le développement de cellules neuronales à partir de l’hippocampe, du cortex et autres régions du cerveau. Le milieu de culture Neurobasal® permet la maintenance à long terme comme à court terme des populations homogènes de cellules neuronales sans nécessiter une couche nourricière d’astrocytes.
Ajouter vos propres suppléments
Tous les milieux de culture Neurobasal® ne contiennent ni L-glutamine, ni acide L-glutamique ni acide aspartique. Les milieux Neurobasal® doivent être supplémentés par 0,5 mM de L-glutamine ou de supplément GlutaMAX™-I et soit un supplément sans sérum (tel que le supplément B-27® soitle supplément N-2), ou avec du sérum. Pour l’étalement initial, vous devez ajouter 25 µM d’acide glutamique.
Test du contrôle qualité
Tous les milieux Neurobasal® sont testés pour vérifier leur pH, leur osmolalité, les endotoxines, et l’absence de contamination bactérienne et fongique.
Le milieu Neurobasal® contient du rouge de phénol. Si le rouge de phénol ne convient pas à vos applications, nous fournissons également un milieu Neurobasal® sans rouge de phénol.
Le milieu de culture Neurobasal® est optimisé pour les neurones prénatals et embryonnaires. Pour la mise en culture de neurones du cerveau post-natals et adultes, nous proposons le milieu A Neurobasal®.
Consultez la gamme complète de produits Neurobasal®.
Pour en savoir plus sur les produits de culture cellulaire neurale d’Invitrogen, rendez-vous sur www.invitrogen.com/neuralculture.
Usage exclusivement réservé à la recherche. Non destiné à des fins thérapeutiques ou diagnostiques humaines ou animales.
Ajouter vos propres suppléments
Tous les milieux de culture Neurobasal® ne contiennent ni L-glutamine, ni acide L-glutamique ni acide aspartique. Les milieux Neurobasal® doivent être supplémentés par 0,5 mM de L-glutamine ou de supplément GlutaMAX™-I et soit un supplément sans sérum (tel que le supplément B-27® soitle supplément N-2), ou avec du sérum. Pour l’étalement initial, vous devez ajouter 25 µM d’acide glutamique.
Test du contrôle qualité
Tous les milieux Neurobasal® sont testés pour vérifier leur pH, leur osmolalité, les endotoxines, et l’absence de contamination bactérienne et fongique.
Le milieu Neurobasal® contient du rouge de phénol. Si le rouge de phénol ne convient pas à vos applications, nous fournissons également un milieu Neurobasal® sans rouge de phénol.
Le milieu de culture Neurobasal® est optimisé pour les neurones prénatals et embryonnaires. Pour la mise en culture de neurones du cerveau post-natals et adultes, nous proposons le milieu A Neurobasal®.
Consultez la gamme complète de produits Neurobasal®.
Pour en savoir plus sur les produits de culture cellulaire neurale d’Invitrogen, rendez-vous sur www.invitrogen.com/neuralculture.
Usage exclusivement réservé à la recherche. Non destiné à des fins thérapeutiques ou diagnostiques humaines ou animales.
B-27™ Plus Supplement (50X) (Gibco™)
B-27 Plus Supplement is the next-generation of the most cited neuronal cell culture product, B-27 Supplement. It is a serum-free supplement that provides the highest rate of in vitro survival of primary rodent and human stem cell-derived neurons when used with Neurobasal Plus Medium. B-27 Plus Supplement features an optimized formulation, upgraded manufacturing process, and more stringent quality control of raw materials and final product. These improvements enable the highest rate of neuronal cell survival, improved electrophysiological activity, and enhanced functional maturity versus other neuronal cell culture media systems.
B-27 Plus Supplement, when combined with Neurobasal Plus Medium, offers:
• At least 50% higher survival rate of primary rat neurons compared to classic B-27 Supplement and Neurobasal Medium
• Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems
• Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium
• Accelerates neurite outgrowth and increases the number of synaptic-positive puncta
Available as a 50X, 10 mL supplement, B-27 Plus Supplement can seamlessly replace other neuronal cell culture supplements, such as classic B-27 Supplement. The B-27 Plus Neuronal Culture System is also available for purchase, which is comprised of B-27 Plus Supplement and Neurobasal Plus Medium, offering a convenient and valuable all-in-one ordering solution.
B-27 Plus Supplement, when combined with Neurobasal Plus Medium, offers:
• At least 50% higher survival rate of primary rat neurons compared to classic B-27 Supplement and Neurobasal Medium
• Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems
• Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium
• Accelerates neurite outgrowth and increases the number of synaptic-positive puncta
Available as a 50X, 10 mL supplement, B-27 Plus Supplement can seamlessly replace other neuronal cell culture supplements, such as classic B-27 Supplement. The B-27 Plus Neuronal Culture System is also available for purchase, which is comprised of B-27 Plus Supplement and Neurobasal Plus Medium, offering a convenient and valuable all-in-one ordering solution.
Neurobasal™ Plus Medium (Gibco™)
Neurobasal Plus Medium is the next-generation of one of the most cited neuronal cell culture products, Neurobasal Medium. It is a neuronal basal medium that provides the highest rates of in vitro survival of primary rodent and human stem cell-derived neurons when used with B-27 Plus Supplement. Neurobasal Plus Medium features an optimized formulation, upgraded manufacturing process, and more stringent quality control of raw materials and final product. These improvements enable the highest rates of neuronal cell survival, improved electrophysiological activity, and enhanced functional maturity versus other neuronal cell culture media systems.
Neurobasal Plus Medium, when combined with B-27 Plus Supplement, offers:
• At least 50% higher survival rate of primary rat neurons compared to classic Neurobasal Medium and B-27 Supplement
• Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems
• Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium
• Accelerates neurite outgrowth and increases the number of synaptic-positive puncta
Available as a 500 mL basal medium, Neurobasal Plus Medium can seamlessly replace other neuronal cell culture media, such as classic Neurobasal Medium. The B-27 Plus Neuronal Culture System is also available for purchase, which is comprised of B-27 Plus Supplement and Neurobasal Plus Medium, offering a convenient and valuable all-in-one ordering solution.
Neurobasal Plus Medium, when combined with B-27 Plus Supplement, offers:
• At least 50% higher survival rate of primary rat neurons compared to classic Neurobasal Medium and B-27 Supplement
• Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems
• Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium
• Accelerates neurite outgrowth and increases the number of synaptic-positive puncta
Available as a 500 mL basal medium, Neurobasal Plus Medium can seamlessly replace other neuronal cell culture media, such as classic Neurobasal Medium. The B-27 Plus Neuronal Culture System is also available for purchase, which is comprised of B-27 Plus Supplement and Neurobasal Plus Medium, offering a convenient and valuable all-in-one ordering solution.
Neurobasal™ Medium, minus phenol red (Gibco™)
Le milieu Neurobasal® sans rouge de phénol est un milieu basal qui satisfait aux exigences particulières des cellules neuronales prénatales et embryonnaires lorsqu’il est utilisé avec le supplément GIBCO® B-27®, mais sans rouge de phénol. Le milieu de culture Neurobasal® peut être utilisé pour le développement de cellules neuronales à partir de l’hippocampe, du cortex et autres régions du cerveau. Le milieu de culture Neurobasal® permet la maintenance à long terme comme à court terme des populations homogènes de cellules neuronales sans nécessiter une couche nourricière d’astrocytes.
Utilisez le milieu de culture Neurobasal® sans rouge de phénol pour les applications dans lesquelles la présence de rouge de phénol interfère avec vos résultats.
Ajouter vos propres suppléments
Tous les milieux de culture Neurobasal® ne contiennent ni L-glutamine, ni acide L-glutamique ni acide aspartique. Les milieux Neurobasal® doivent être supplémentés par 0,5 mM de L-glutamine ou de supplément GlutaMAX™-I et soit un supplément sans sérum (tel que le supplément B-27® soitle supplément N-2), ou avec du sérum. Pour l’étalement initial, vous devez ajouter 25 µM d’acide glutamique.
Test du contrôle qualité
Tous les milieux Neurobasal® sont testés pour vérifier leur pH, leur osmolalité, les endotoxines, et l’absence de contamination bactérienne et fongique.
Nous fournissons également le milieu de culture Neurobasal® contenant du rouge de phénol.
Le milieu de culture Neurobasal® est optimisé pour les neurones prénatals et embryonnaires. Pour la mise en culture de neurones du cerveau post-natals et adultes, nous proposons le milieu de culture Neurobasal®-A.
Consultez la gamme complète de produits Neurobasal®.
Pour en savoir plus sur les produits de culture cellulaire neurale d’Invitrogen, rendez-vous sur www.invitrogen.com/neuralculture.
Usage exclusivement réservé à la recherche. Non destiné à des fins thérapeutiques ou diagnostiques humaines ou animales.
Utilisez le milieu de culture Neurobasal® sans rouge de phénol pour les applications dans lesquelles la présence de rouge de phénol interfère avec vos résultats.
Ajouter vos propres suppléments
Tous les milieux de culture Neurobasal® ne contiennent ni L-glutamine, ni acide L-glutamique ni acide aspartique. Les milieux Neurobasal® doivent être supplémentés par 0,5 mM de L-glutamine ou de supplément GlutaMAX™-I et soit un supplément sans sérum (tel que le supplément B-27® soitle supplément N-2), ou avec du sérum. Pour l’étalement initial, vous devez ajouter 25 µM d’acide glutamique.
Test du contrôle qualité
Tous les milieux Neurobasal® sont testés pour vérifier leur pH, leur osmolalité, les endotoxines, et l’absence de contamination bactérienne et fongique.
Nous fournissons également le milieu de culture Neurobasal® contenant du rouge de phénol.
Le milieu de culture Neurobasal® est optimisé pour les neurones prénatals et embryonnaires. Pour la mise en culture de neurones du cerveau post-natals et adultes, nous proposons le milieu de culture Neurobasal®-A.
Consultez la gamme complète de produits Neurobasal®.
Pour en savoir plus sur les produits de culture cellulaire neurale d’Invitrogen, rendez-vous sur www.invitrogen.com/neuralculture.
Usage exclusivement réservé à la recherche. Non destiné à des fins thérapeutiques ou diagnostiques humaines ou animales.
Astrocyte Medium (Gibco™)
GIBCO® Astrocyte Medium has been specially formulated to support the growth of primary human and rat astrocytes while retaining their phenotypes. The medium has been internally validated and optimized to provide an optimal balance between cell growth and the maintenance of cell viability, normal phenotype, and function. If more rapid proliferation is desired, addition of an appropriate amount of epidermal growth factor (EGF) is recommended, although cells may exhibit morphological and phenotypic changes.
Astrocytes are a type of glial cells of the nervous system with a typical star shape. It has been discovered in recent years that astrocytes not only function as gap fillers and structural supports, but play an active role in supporting the normal functions of neurons, oligodendrocytes, neural stem cells, blood-brain barrier, and so on. They regulate the extracellular ionic and chemical environment, respond to CNS injury, and play a fundamental role in the pathogenesis of ischemic neuronal death, and they have remained as one of the most active research areas in neuroscience.
GIBCO® Astrocyte Medium has three components: base medium (D MEM), N-2 Supplement, and OneShot™ Fetal Bovine Serum (FBS). Mixing of these three components yields a complete medium.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated.
Astrocytes are a type of glial cells of the nervous system with a typical star shape. It has been discovered in recent years that astrocytes not only function as gap fillers and structural supports, but play an active role in supporting the normal functions of neurons, oligodendrocytes, neural stem cells, blood-brain barrier, and so on. They regulate the extracellular ionic and chemical environment, respond to CNS injury, and play a fundamental role in the pathogenesis of ischemic neuronal death, and they have remained as one of the most active research areas in neuroscience.
GIBCO® Astrocyte Medium has three components: base medium (D MEM), N-2 Supplement, and OneShot™ Fetal Bovine Serum (FBS). Mixing of these three components yields a complete medium.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated.
Cell Therapy Systems Hibernate™-E CTS™ Medium (Gibco™)
Cell Therapy Systems Hibernate®-E CTS™ media is a serum-free, nutrient basal media for the short-term maintenance of embryonic tissue in ambient CO2 conditions (Ref 1). Human tissues maintained in Hibernate®-E media include brain tumors (Ref 2), umbilical cords, and foreskins.
Quick and Easy Shipment of Human Tissues
Cell Therapy Systems Hibernate®-E CTS™ media can be used for shipment of human embryonic tissue as brain tumor, umbilical cord, and foreskin at ambient temperature using a simple 3 step process:
Collect tissue in a conical tube containing Hibernate medium (brain tumor cells require additional supplements).
• Ship tissues in Hibernate® media within 24 to 72 hours on cold packs.
• Transfer received tissues into media of choice and initiate cultivation.
Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)
References:
• Brewer, G.J. and Price, P.J. (1996).Viable cultured neurons in ambient carbon dioxide and hibernation storage for a month. Neuroreports 7: 1509-1512.
• Brewer, G.J. and LeRoux, P.D.(2007). Human primary brain tumor cell growth inhibition in serum-free medium optimized for neuron survival. Brain Res. 1157:156-66.
Quick and Easy Shipment of Human Tissues
Cell Therapy Systems Hibernate®-E CTS™ media can be used for shipment of human embryonic tissue as brain tumor, umbilical cord, and foreskin at ambient temperature using a simple 3 step process:
Collect tissue in a conical tube containing Hibernate medium (brain tumor cells require additional supplements).
• Ship tissues in Hibernate® media within 24 to 72 hours on cold packs.
• Transfer received tissues into media of choice and initiate cultivation.
Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)
References:
• Brewer, G.J. and Price, P.J. (1996).Viable cultured neurons in ambient carbon dioxide and hibernation storage for a month. Neuroreports 7: 1509-1512.
• Brewer, G.J. and LeRoux, P.D.(2007). Human primary brain tumor cell growth inhibition in serum-free medium optimized for neuron survival. Brain Res. 1157:156-66.
CTS™ Neurobasal™ Medium (Gibco™)
CTS™ (Cell Therapy Systems) Neurobasal® Medium is a serum-free basal medium for the long-term viability of prenatal and embryonic hippocampal and other neurons of the CNS. When used with B-27® Supplement, Neurobasal® Medium CTS™ media can cultivate human glioblastoma, neurons, neural stem cells, and non-CNS lineage cell types with good viability and survival. Neurobasal® Medium CTS™ media supplemented with B-27® supplement contains anti-oxidants to reduce reactive oxygen damage, and no glutamate and aspartate amino acids, allowing for study of excitatory neurotransmitters.
Media Preparation
Prior to use with glioblastoma or neural stem cells, Cell Therapy Systems Neurobasal® Medium CTS™ media must be supplemented with L-glutamine or GlutaMAX™-I CTS™ supplement. In addition, either a serum-free supplement or serum needs to be added. Our recommended supplement for human cells is B-27® Supplement (Cat. No. 17504).
Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)
For Research Use or Non-Commercial Manufacturing of Cell-Based Products for Clinical Research.
CAUTION: Not intended for direct administration to humans or animals
Media Preparation
Prior to use with glioblastoma or neural stem cells, Cell Therapy Systems Neurobasal® Medium CTS™ media must be supplemented with L-glutamine or GlutaMAX™-I CTS™ supplement. In addition, either a serum-free supplement or serum needs to be added. Our recommended supplement for human cells is B-27® Supplement (Cat. No. 17504).
Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)
For Research Use or Non-Commercial Manufacturing of Cell-Based Products for Clinical Research.
CAUTION: Not intended for direct administration to humans or animals
Hibernate™-A Medium (Gibco™)
Le milieu Hibernate® est un milieu de nutriants, indépendant du CO2 et destiné à la maintenance des cellules, des tissus et des coupes de tissus neuraux. Hibernate®-A est le milieu de nutriants pour les cellules et les tissus adultes et postnataux dans des niveaux de dioxyde de carbone ambiant ; Hibernate®-E est le milieu de nutriants pour les cellules ou tissus embryonnaires dans des niveaux de dioxyde de carbone ambiant. La première application permet une période de maintenance plus longue (jusqu’à 48 heures) de la viabilité des neurones dans le CO2 ambiant. Ceci donne aux scientifiques plus de confort et de contrôle lors de l’exécution d’expériences avec des cellules vivantes sur la paillasse, telles que les études de microscopie, la cytométrie de flux et d’autres études physiologiques. La deuxième application du milieu Hibernate® concerne la conservation des tissus cérébraux viables avant traitement, et ce pendant un mois maximum, avec réfrigération et maintenance des coupes de tissus.
• Systèmes de milieux optimisés pour le stockage viable de tissus et de coupes neuraux, pendant un mois maximum, entre 2 et 8°C ; systèmes pratiques qui permettent aux scientifiques de conserver des tissus ⁄ coupes avant traitement
• Maintenance de la viabilité et des fonctions des cellules neuronales à des niveaux de CO2 ambiant pendant 48 heures maximum ; rend possible l’exécution d’expériences avec des cellules vivantes sans avoir recours à une chambre à CO2 coûteuse adaptée à l’instrument
• Fabriqué conformément aux BPFa
Les produits GIBCO® Hibernate®-A et Hibernate®-E sont des milieux de nutriants indépendants du CO2 destinés à la maintenance des cellules neurales et la conservation de tissus. Il est fourni sous forme de milieu 1X et le mélange du Hibernate® avec le supplément GIBCO® B-27® (50X) produit un milieu complet.
Usage exclusivement réservé à la recherche. Le supplément n’est pas prévu à des fins thérapeutiques humaines ou animales ni pour une utilisation diagnostique, sauf indication contraire.
Références :
1. Viable cultured neurons in ambient carbon dioxide and hibernation storage for a month.Brewer GJ, Price P J.Neuroreport. 17 juin 1996 ; 7(9):1509-12.
2. Serum-free culture of rat post-natal and fetal brainstem neurons.Kivell BM, McDonald FJ, Miller JH. Brain Res Dev Brain Res. 14 avril 2000 ; 120(2):199-210.
3. Method for serum-free culture of late fetal and early postnatal rat brainstem neurons.Kivell BM, McDonald FJ, Miller JH. Brain Res Brain Res Protoc. Février 2001 ; 6(3):91-9.
4. Human primary brain tumor cell growth inhibition in serum-free medium optimized for neuron survival.Brewer GJ, LeRoux PD. Brain Res. 9 juillet 2007 ; 1157:156-66.
5. Survival and stimulation of neurite outgrowth in a serum-free culture of spiral ganglion neurons from adult mice.Vieira M, Christensen BL, Wheeler BC, Feng AS, Kollmar R. Hear Res. Août 2007 ; 230(1-2):17-23.
6. Dopamine D1-like receptor activation induces brain-derived neurotrophic factor protein expression.Williams SN, Undieh AS. Neuroreport. 22 avril 2009 ; 20(6):606-10.
7. Neonatal desensitization allows long-term survival of neural xenotransplants without immunosuppression.Kelly CM, Precious SV, Scherf C, Penketh R, Amso NN, Battersby A, Allen ND, Dunnett SB, Rosser AE. Nature Methods. Avril 2009 ; 6(4):271-3.
• Systèmes de milieux optimisés pour le stockage viable de tissus et de coupes neuraux, pendant un mois maximum, entre 2 et 8°C ; systèmes pratiques qui permettent aux scientifiques de conserver des tissus ⁄ coupes avant traitement
• Maintenance de la viabilité et des fonctions des cellules neuronales à des niveaux de CO2 ambiant pendant 48 heures maximum ; rend possible l’exécution d’expériences avec des cellules vivantes sans avoir recours à une chambre à CO2 coûteuse adaptée à l’instrument
• Fabriqué conformément aux BPFa
Les produits GIBCO® Hibernate®-A et Hibernate®-E sont des milieux de nutriants indépendants du CO2 destinés à la maintenance des cellules neurales et la conservation de tissus. Il est fourni sous forme de milieu 1X et le mélange du Hibernate® avec le supplément GIBCO® B-27® (50X) produit un milieu complet.
Usage exclusivement réservé à la recherche. Le supplément n’est pas prévu à des fins thérapeutiques humaines ou animales ni pour une utilisation diagnostique, sauf indication contraire.
Références :
1. Viable cultured neurons in ambient carbon dioxide and hibernation storage for a month.Brewer GJ, Price P J.Neuroreport. 17 juin 1996 ; 7(9):1509-12.
2. Serum-free culture of rat post-natal and fetal brainstem neurons.Kivell BM, McDonald FJ, Miller JH. Brain Res Dev Brain Res. 14 avril 2000 ; 120(2):199-210.
3. Method for serum-free culture of late fetal and early postnatal rat brainstem neurons.Kivell BM, McDonald FJ, Miller JH. Brain Res Brain Res Protoc. Février 2001 ; 6(3):91-9.
4. Human primary brain tumor cell growth inhibition in serum-free medium optimized for neuron survival.Brewer GJ, LeRoux PD. Brain Res. 9 juillet 2007 ; 1157:156-66.
5. Survival and stimulation of neurite outgrowth in a serum-free culture of spiral ganglion neurons from adult mice.Vieira M, Christensen BL, Wheeler BC, Feng AS, Kollmar R. Hear Res. Août 2007 ; 230(1-2):17-23.
6. Dopamine D1-like receptor activation induces brain-derived neurotrophic factor protein expression.Williams SN, Undieh AS. Neuroreport. 22 avril 2009 ; 20(6):606-10.
7. Neonatal desensitization allows long-term survival of neural xenotransplants without immunosuppression.Kelly CM, Precious SV, Scherf C, Penketh R, Amso NN, Battersby A, Allen ND, Dunnett SB, Rosser AE. Nature Methods. Avril 2009 ; 6(4):271-3.
Neurobasal™-A Medium, minus phenol red (Gibco™)
Neurobasal®-A Medium Minus Phenol Red is a basal media that meets the special requirements of post-natal and adult brain neuronal when used with GIBCO® B-27® Supplement, but without Phenol Red. Neurobasal®-A Medium can be used to grow neuronal cells from hippocampus, cortex and other regions of the brain. Neurobasal®-A Medium allows for both long and short term maintenance of homogeneous populations of neuronal cells without the need of an astrocyte feeder layer.
Use Neurobasal®-A Medium Minus Phenol Red for applications in which the presence of Phenol Red will interfere with your results.
Add Your Own Supplements
All Neurobasal® media contain no L-glutamine, L-glutamic acid, or aspartic acid. Neurobasal® media must be supplemented with 0.5 mM L-glutamine or GlutaMAX™-I Supplement and either a serum-free supplement (such as B-27® Supplement or N-2 Supplement), or with serum. For initial plating, you should add 25 µM glutamic acid.
Quality Control Testing
All Neurobasal® media are tested for pH, osmolality, endotoxin, and the absence of bacterial and fungal contamination.
We also provide Neurobasal® -A Medium with Phenol Red.
Neurobasal®-A Medium is optimized for post-natal and adult brain neurons. For growing pre-natal and embryonic neurons, we provide Neurobasal® Medium.
See the entire Neurobasal® product line.
Learn more about neural cell culture products from Invitrogen at www.invitrogen.com/neuralculture.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Use Neurobasal®-A Medium Minus Phenol Red for applications in which the presence of Phenol Red will interfere with your results.
Add Your Own Supplements
All Neurobasal® media contain no L-glutamine, L-glutamic acid, or aspartic acid. Neurobasal® media must be supplemented with 0.5 mM L-glutamine or GlutaMAX™-I Supplement and either a serum-free supplement (such as B-27® Supplement or N-2 Supplement), or with serum. For initial plating, you should add 25 µM glutamic acid.
Quality Control Testing
All Neurobasal® media are tested for pH, osmolality, endotoxin, and the absence of bacterial and fungal contamination.
We also provide Neurobasal® -A Medium with Phenol Red.
Neurobasal®-A Medium is optimized for post-natal and adult brain neurons. For growing pre-natal and embryonic neurons, we provide Neurobasal® Medium.
See the entire Neurobasal® product line.
Learn more about neural cell culture products from Invitrogen at www.invitrogen.com/neuralculture.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Gibco™ Neural Cell Culture Starter Kit (Gibco™)
The Gibco™ Neural Cell Culture Starter Kit provides researchers with a convenient package of reagents for the culture of a wide variety of neural cell types.
Whether you are culturing neural cell lines, primary neurons, or neural stem cells, having the right components for your complete neural cell culture medium is essential to achieving the best cell culture results.
This starter kit includes the following products for neural cell culture:
• B-27™ Serum-free Supplement (50X), 10 mL
• GlutaMAX™ Supplement, 100 mL
• Neurobasal™ Medium, 500 mL
Whether you are culturing neural cell lines, primary neurons, or neural stem cells, having the right components for your complete neural cell culture medium is essential to achieving the best cell culture results.
This starter kit includes the following products for neural cell culture:
• B-27™ Serum-free Supplement (50X), 10 mL
• GlutaMAX™ Supplement, 100 mL
• Neurobasal™ Medium, 500 mL
Cell Therapy Systems Hibernate™-A CTS™ Medium (Gibco™)
Cell Therapy Systems Hibernate®-A CTS™ media is a serum-free, nutrient basal media for the short-term maintenance of postnatal and adult tissue human tissue in ambient CO2 conditions (Ref 1). Human tissues maintained in Hibernate®-A media include brain tumors (Ref 2), umbilical cords, and foreskins.
Quick and Easy Shipment of Human Tissues
Cell Therapy Systems Hibernate®-A CTS™ media can be used for shipment of human postnatal and adult tissue such as brain tumor, umbilical cord, and foreskin at ambient temperature using a simple 3 step process:
Collect tissue in a conical tube containing Hibernate medium (brain tumor cells require additional supplements).
• Ship tissues in Hibernate® media within 24 to 72 hours on cold packs.
• Transfer received tissues into media of choice and initiate cultivation.
Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)
For Research Use or Non-Commercial Manufacturing of Cell-Based Products for Clinical Research.
CAUTION: Not intended for direct administration to humans or animals
References:
• Brewer, G.J. and Price, P.J. (1996).Viable cultured neurons in ambient carbon dioxide and hibernation storage for a month. Neuroreports 7: 1509-1512.
• Brewer, G.J. and LeRoux, P.D.(2007). Human primary brain tumor cell growth inhibition in serum-free medium optimized for neuron survival. Brain Res. 1157:156-66.
Quick and Easy Shipment of Human Tissues
Cell Therapy Systems Hibernate®-A CTS™ media can be used for shipment of human postnatal and adult tissue such as brain tumor, umbilical cord, and foreskin at ambient temperature using a simple 3 step process:
Collect tissue in a conical tube containing Hibernate medium (brain tumor cells require additional supplements).
• Ship tissues in Hibernate® media within 24 to 72 hours on cold packs.
• Transfer received tissues into media of choice and initiate cultivation.
Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)
For Research Use or Non-Commercial Manufacturing of Cell-Based Products for Clinical Research.
CAUTION: Not intended for direct administration to humans or animals
References:
• Brewer, G.J. and Price, P.J. (1996).Viable cultured neurons in ambient carbon dioxide and hibernation storage for a month. Neuroreports 7: 1509-1512.
• Brewer, G.J. and LeRoux, P.D.(2007). Human primary brain tumor cell growth inhibition in serum-free medium optimized for neuron survival. Brain Res. 1157:156-66.
CTS™ Neurobasal™-A Medium (Gibco™)
CTS™ (Cell Therapy Systems) Neurobasal® A Medium is a serum-free basal medium for the long-term viability of postnatal and adult hippocampal and other neurons of the CNS. When used with B-27® Supplement, Neurobasal® A Medium CTS™ media can cultivate human glioblastoma, neurons, neural stem cells, and non-CNS lineage cell types with good viability and survival. Neurobasal® A Medium CTS™ media supplemented with B-27® supplement contains anti-oxidants to reduce reactive oxygen damage, and no glutamate and aspartate amino acids, allowing for study of excitatory neurotransmitters.
Media Preparation
Prior to use with glioblastoma or neural stem cells, Cell Therapy Systems Neurobasal® A Medium CTS™ media must be supplemented with L-glutamine or GlutaMAX™-I CTS™ supplement. In addition, either a serum-free supplement or serum needs to be added. Our recommended supplement for human cells is B-27® Supplement (Cat. No. 17504).
Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)
For Research Use or Non-Commercial Manufacturing of Cell-Based Products for Clinical Research.
CAUTION: Not intended for direct administration to humans or animals
Media Preparation
Prior to use with glioblastoma or neural stem cells, Cell Therapy Systems Neurobasal® A Medium CTS™ media must be supplemented with L-glutamine or GlutaMAX™-I CTS™ supplement. In addition, either a serum-free supplement or serum needs to be added. Our recommended supplement for human cells is B-27® Supplement (Cat. No. 17504).
Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)
For Research Use or Non-Commercial Manufacturing of Cell-Based Products for Clinical Research.
CAUTION: Not intended for direct administration to humans or animals
B-27™ Electrophysiology Kit (Gibco™)
The B-27® Electrophysiology Kit contains Neurobasal® Medium, Electro and B-27® Supplement, Electro, both specifically developed to enhance network spike rates in cultured neurons when compared with other media and supplement combinations. Electrophysiology is the fundamental technique to assess such electrical activities to study the functions and dysfunctions in cultured neurons.
Using the B-27® Electrophysiology Kit in your neuronal studies offers:
• Increased synapse densities
• More spontaneous spike rates
• Improved multielectrode array efficiency
More Synapses, More Signal, Simpler Testing
The B-27® Electrophysiology Kit is an improvement to Neurobasal® and B-27® combination for cultured networks. Hippocampal and cortical neurons cultured in this media system show an increased density of synapses and neurotransmitter receptors which produce higher spike rates in neural networks, helping to simplifying your study.
Relevant Results for Your Electrophysiology Studies
Using Invitrogen® B-27® Electrophysiology Kit, allows you to get more relevant results when studying neuron physiology and the biochemical basis of normal and disease processes. The B-27® Electrophysiology Kit can yield better results in other electrophysiological studies, including neurons used in multielectrode arrays in neurotoxicology and neuropharmacology studies.
For other applications, we offer alternative formulations of B-27® and Neurobasal®.
Using the B-27® Electrophysiology Kit in your neuronal studies offers:
• Increased synapse densities
• More spontaneous spike rates
• Improved multielectrode array efficiency
More Synapses, More Signal, Simpler Testing
The B-27® Electrophysiology Kit is an improvement to Neurobasal® and B-27® combination for cultured networks. Hippocampal and cortical neurons cultured in this media system show an increased density of synapses and neurotransmitter receptors which produce higher spike rates in neural networks, helping to simplifying your study.
Relevant Results for Your Electrophysiology Studies
Using Invitrogen® B-27® Electrophysiology Kit, allows you to get more relevant results when studying neuron physiology and the biochemical basis of normal and disease processes. The B-27® Electrophysiology Kit can yield better results in other electrophysiological studies, including neurons used in multielectrode arrays in neurotoxicology and neuropharmacology studies.
For other applications, we offer alternative formulations of B-27® and Neurobasal®.
Hibernate™-E Medium (Gibco™)
Hibernate® is a CO2-independent nutrient medium for the maintenance of neural cells, tissue, and tissue slices. Hibernate®-A is the nutrient medium for adult and postnatal cells or tissue in ambient carbon dioxide levels; Hibernate®-E is the nutrient medium for embryonic cells or tissue in ambient carbon dioxide levels. The first application allows for longer period (up to 48 hours) maintenance of neuron viability in ambient CO2. This gives scientists greater convenience and control running bench-top live cell experiments, such as microscopy studies, flow cytometry, and other physiology studies. The second application of Hibernate® is for the preservation of viable brain tissue before processing for up to a month with refrigeration and maintenance of tissue slices.
• Optimized media systems for the viable storage of neural tissue and slices for up to 1 month at 2 - 8 °C - convenient systems that allow scientists to preserve tissue⁄slices before processing
• Maintenance of neuronal cells viability and functions at ambient CO2 level for up to 48 hours - makes conducting live-cell experiments possible without an expensive CO2 chamber adapted to instrument
• Manufactured under cGMP.
GIBCO® Hibernate®-A and Hibernate®-E are CO2-independent nutrient media for the maintenance of neural cells and preservation of tissue. It is supplied as 1X medium and mixing of Hibernate® with GIBCO® B-27® Supplement (50X) yields a complete medium.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated.
References:
1. Viable cultured neurons in ambient carbon dioxide and hibernation storage for a month.Brewer GJ, Price P J.Neuroreport. 1996 Jun 17;7(9):1509-12.
2. Serum-free culture of rat post-natal and fetal brainstem neurons.Kivell BM, McDonald FJ, Miller JH. Brain Res Dev Brain Res. 2000 Apr 14;120(2):199-210.
3. Method for serum-free culture of late fetal and early postnatal rat brainstem neurons.Kivell BM, McDonald FJ, Miller JH. Brain Res Brain Res Protoc. 2001 Feb;6(3):91-9.
4. Human primary brain tumor cell growth inhibition in serum-free medium optimized for neuron survival.Brewer GJ, LeRoux PD. Brain Res. 2007 Jul 9;1157:156-66.
5. Survival and stimulation of neurite outgrowth in a serum-free culture of spiral ganglion neurons from adult mice.Vieira M, Christensen BL, Wheeler BC, Feng AS, Kollmar R. Hear Res. 2007 Aug;230(1-2):17-23.
6. Dopamine D1-like receptor activation induces brain-derived neurotrophic factor protein expression.Williams SN, Undieh AS. Neuroreport. 2009 Apr 22;20(6):606-10.
7. Neonatal desensitization allows long-term survival of neural xenotransplants without immunosuppression.Kelly CM, Precious SV, Scherf C, Penketh R, Amso NN, Battersby A, Allen ND, Dunnett SB, Rosser AE. Nature Methods. 2009 Apr;6(4):271-3.
• Optimized media systems for the viable storage of neural tissue and slices for up to 1 month at 2 - 8 °C - convenient systems that allow scientists to preserve tissue⁄slices before processing
• Maintenance of neuronal cells viability and functions at ambient CO2 level for up to 48 hours - makes conducting live-cell experiments possible without an expensive CO2 chamber adapted to instrument
• Manufactured under cGMP.
GIBCO® Hibernate®-A and Hibernate®-E are CO2-independent nutrient media for the maintenance of neural cells and preservation of tissue. It is supplied as 1X medium and mixing of Hibernate® with GIBCO® B-27® Supplement (50X) yields a complete medium.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated.
References:
1. Viable cultured neurons in ambient carbon dioxide and hibernation storage for a month.Brewer GJ, Price P J.Neuroreport. 1996 Jun 17;7(9):1509-12.
2. Serum-free culture of rat post-natal and fetal brainstem neurons.Kivell BM, McDonald FJ, Miller JH. Brain Res Dev Brain Res. 2000 Apr 14;120(2):199-210.
3. Method for serum-free culture of late fetal and early postnatal rat brainstem neurons.Kivell BM, McDonald FJ, Miller JH. Brain Res Brain Res Protoc. 2001 Feb;6(3):91-9.
4. Human primary brain tumor cell growth inhibition in serum-free medium optimized for neuron survival.Brewer GJ, LeRoux PD. Brain Res. 2007 Jul 9;1157:156-66.
5. Survival and stimulation of neurite outgrowth in a serum-free culture of spiral ganglion neurons from adult mice.Vieira M, Christensen BL, Wheeler BC, Feng AS, Kollmar R. Hear Res. 2007 Aug;230(1-2):17-23.
6. Dopamine D1-like receptor activation induces brain-derived neurotrophic factor protein expression.Williams SN, Undieh AS. Neuroreport. 2009 Apr 22;20(6):606-10.
7. Neonatal desensitization allows long-term survival of neural xenotransplants without immunosuppression.Kelly CM, Precious SV, Scherf C, Penketh R, Amso NN, Battersby A, Allen ND, Dunnett SB, Rosser AE. Nature Methods. 2009 Apr;6(4):271-3.
